International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2020.447.459Ligusticum chuanxiong Hort Extract and Borneol in Protecting Brain Microvascular Endothelial Cells against Oxygen-Glucose Deprivation/Reperfusion Injury]]>YuBin YaoYao ZhangXiaofeng XuHuiqin LuJinfu RuanMing 62020166Background and Objective:Ligusticum chuanxiong Hort (LC) and borneol (BO) are usually prescribed as a combination of ischemic stroke patients for a better therapeutic effect in China. However, their synergic mechanism is unclear. Considering the critical role of Brain Microvascular Endothelial Cells (BMECs) in this disorder, the present study was designed to explore their synergic mechanism in protecting BMECs against Oxygen-Glucose Deprivation/Reperfusion (OGD/R) injury. Materials and Methods: The primary cultured BMECs were identified and divided into 5 groups of control, model, ELC (50 mg L1), BO (50 μg L1) and ELC+BO. Then their synergic treatment was evaluated via measuring oxidative stress, [Ca2+]i, apoptosis ratio, levels of apoptosis-related genes and angiogenesis-related proteins. Results: After a comparison between the combined group and their monotherapies, it was shown that the superiority of ELC was in inhibiting oxidative stress and apoptosis, regulating [Ca2+]i and levels of apoptosis-related genes, while that of BO was in promoting angiogenesis. Interestingly, the combined therapy even reduced VEGFR1 expression which was unaffected in both of their monotherapies. Conclusion: ELC and BO might play different roles in protecting BMECs against oxygen-glucose deprivation/reperfusion injury and their synergic effect was displayed in the present study.]]>Katan, M. and A. Luft,201838208211Beck H. and K. H. Plate2009117481496Chen J. and M. Chopp,20073466473Huang S., T. Gong, T. Zhang, X. Wang, Q. Cheng and Y. Li,201842222230Yang, X., X. Zeng and T. Wu,20162016Ran, X., L. Ma, C. Peng, H. Zhang and L.P. Qin,2011Ligusticum chuanxiong Hort: A review of chemistry and pharmacology.]]>4911801189Chen, Z., C. Zhang, F. Gao, Q. Fu, C. Fu, Y. He and J. Zhang,2018Ligusticum chuanxiong Hort. (Chuanxiong).]]>119309325Chang, L., C.Y. Yin, H.Y. Wu, B.B. Tian, Y. Zhu, C.X. Luo and Dong-YaZhu,201731306314Zhang, X.G., C. Shan, J.Z. Zhu, X.Y. Bao and Q. Tong et al.,20182018Yu, B., M. Ruan, X.B. Cui, J.M. Guo, L. Xu and X.P. Dong,201377128132Yu, B., M. Ruan, Y. Sun, X. Cui, Y. Yu, L. Wang and T. Fang,2011618761882Yu, B., M. Ruan, X. Dong, Y. Yu and H. Cheng,201315010961108Kong, Q.X., Z.Y. Wu, X. Chu, R.Q. Liang, M. Xia and L. Li,201311161164Wu, H.Y., Y. Tang, L.Y. Gao, W.X. Sun and Y. Hua et al.,2014740522531Liu, R., L. Zhang, X. Lan, L. Li, T.T. Zhang, J.H. Sun and G.H. Du,2011176408419Dong, T., N. Chen, X. Ma, J. Wang, J. Wen, Q. Xie and R. Ma,2018102874883Yu B., M. Ruan, T. Liang, Y. Yu,2020Ligusticum chuanxiong Hort Against Cortex and Striatum Ischemia.]]>16104119Liu Y., Q. Xue, Q. Tang, M. Hou, H. Qi, G. Chen, W. Chen, J. Zhang, Y. Chen and X. Xu,201390199205Bocci G.,20027771777Lathey J. L., C. A. Wiley, M. A. Verity and J. A. Nelson,2004176266273Yang H., X. Xi, B. Zhao, Z. Su and Z. Wang,201749523762382Hamanaka, R.B. and N.S. Chandel,201035505513Sena, L.A. and N.S. Chandel,201248158167Peng T. I. and M. J. Jou,20101201183188Szydlowska, K. and M. Tymianski,201047122129Yang J.,20181236267Lei W., J. Ni, X. Xia, M. Jiang and G. Bai,20181092220227Jahani-Asl A., M. Germain and R. S. Slack20091802162166Wang, G.H., R. Lan, X.D. Zhen, W. Zhang, J. Xiang and D.F. Cai,2014154156162Zhang, Y., R. Lan, J. Wang, X.Y. Li and D.N. Zhu et al.,2015172124132Hu Y., M. Duan, S. Liang, Y. Wang and Y. Feng,201516053948Li Y., P. Song, Q. Zhu, Q. Y. Yin, J. W. Ji, Wei Li, H. M. Bian,20143512571264Plate K. H.,200858313320CHEN Z., X. H. XU and J. HU201663173182Ferrara, N., H.P. Gerber and J. LeCouter,20039669676Zhang, Z. and B. Zhou,20132013Ferrara, N.,200425581611Sato Y.,200828604605Woad K. J., A. J. Hammond, M. Hunter, G. E. Mann, M. G. Hunter and R. S. Robinson,2009138581588S. Liao, L. Han, X. Zheng, X. Wang, P. Zhang, J. Wu, R. Liu, Y. Fu, J. Sun, X. Kang, K. Liu, T. P. Fan, S. Li, X. Zheng,201917631433160Mu Q. C., H. Y. Huang, H. J. Gao, P. F. Liu, P. W. Li, Z. Y. Huang, F. B. Yu, T. Lei, Y. Chen and Y. Cheng,201510832840