International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2019.844.850ZhangYonghai LiJunzhao ZhangXianfeng GaoGuangzhong LuJun ZhangQin DingYasuo 72019157Background and Objectives: Malignant glioma is the frequently detected brain tumor with high morbidity and mortality rates. The present study investigated the effect of baicalein, a chemical constituent of Scutellaria baicalensis on glioma cell proliferation. Materials and Methods: The MTT assay was used to determine changes in C6 and U87 glioma cell proliferation by baicalein. Apoptosis induction in U87 cells by baicalein treatment was assessed by commercially available Annexin V-FITC Detection kit (BD Biosciences, San Jose, CA, USA). Effect of baicalein on Mcl-1, caspase-3, PARP and cytochrome c proteins was analyzed by western blotting. Results: The results revealed that baicalein exposure of C6 and U87cells significantly (p<0.05) decreased cell viability. Baicalein treatment of U87 cells caused significant enhancement in apoptotic cell percentage and lead to release of cytochrome c from mitochondria. Its exposure caused cleavage of caspase-3, enhanced PARP activation and increased percentage of cells in G2/M phase. Baicalein treatment caused concentration dependent decrease in myeloid cell leukemia-1 (Mcl-1) protein expression in U87 cells. Conclusion: The study concluded that baicalein treatment inhibits glioma cell viability through induction of apoptosis and arrest of cell cycle. It inhibited Mcl-1 and anti-apoptotic protein expression. Therefore, baicalein can be of immense significance for glioma treatment.]]>Curran, Jr. W.J., C.B. Scott, J. Horton, J.S. Nelson and A.S. Weinstein et al.,199385704710Maher, E.A., F.B. Furnari, R.M. Bachoo, D.H. Rowitch, D.N. Louis and W.K. Cavenee, R.A. DePinho,20011513111333Chang, S.M., K.R. Lamborn, M. Malec, D. Larson and W. Wara et al.,200460353357Fine, H.A., P.Y. Wen, E.A. Maher, E. Viscosi and T. Batchelor et al.,20032122992304Marx, G.M., N. Pavlakis, S. McCowatt, F.M. Boyle and J.A. Levi et al.,2001543138Eramo, A., L. Ricci-Vitiani, A. Zeuner, R. Pallini and F. Lotti et al.,20061312381241Strik, H., M. Deininger, J. Streffer, E. Grote and J. Wickboldt et al.,199967763768Stegh, A.H., H. Kim, R.M. Bachoo, K.L. Forloney and J. Zhang et al.,20072198111Kozopas, K.M., T. Yang, H.L. Buchan, P. Zhou and R.W. Craig,19939035163520Zhang, B., I. Gojo and R.G. Fenton,20029918851893Strauss, S.J., K. Higginbottom, S. Juliger, L. Maharaj and P. Allen et al.,20076727832790Fribley, A. and C.Y. Wang,20065745748Lioni, M., K. Noma, A. Snyder, A. Klein-Szanto and J.A. Diehl et al.,2008728662875Zhang, L., H. Lopez, N.M. George, X. Liu, X. Pang and X. Luo,201118864873Chen, Z., K. Nihei, H. Tanaka, Y. Uda and Y. Kabuyama,2013Scutellariae radix.]]>77657659Yoon, S.B., Y.J. Lee, S.K. Park, H.C. Kim and H. Bae et al.,2009Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages.]]>125286290Ho, F.M., Y.H. Liao, A.J. Yang, P.D.L. Chao and Y.C. Hou et al.,2012142175187Lee, J.J., T. Kim, W.K. Cho and J.Y. Ma,20132013Yue, G.G.L., B.C.L. Chan, H.F. Kwok, M.H. To and K.L. Hon et al.,201226915925Huang, W.H., P.Y. Chien, C.H. Yang and A.R. Lee,2003Scutellaria baicalensis GEORGI.]]>51339340Lee, H.Z., H.W.C. Leung, M.Y. Lai and C.H. Wu,200525959964Domina, A.M., J.H. Smith and R.W. Craig,20002/M accumulation or protein phosphatase 1/2A inhibition.]]>2752168821694Ma, M.H., H.H. Yang, K. Parker, S. Manyak and J.M. Friedman et al.,2003911361144Gomez-Bougie, P., S. Wuilleme-Toumi, E. Menoret, V. Trichet and N. Robillard et al.,20076754185424Lesinski, G.B., E.T. Raig, K. Guenterberg, L. Brown and M.R. Go et al.,20086883518360Quinn, B.A., R. Dash, B. Azab, S. Sarkar and S.K. Das et al.,20112013971411Le Gouill, S., K. Podar, J.L. Harousseau and K.C. Anderson,2004312591262Derenne, S., B. Monia, N.M. Dean, J.K. Taylor and M.J. Rapp et al.,2002L is an essential survival protein of human myeloma cells.]]>100194199Clohessy, J.G., J. Zhuang, J. de Boer, G. Gil-Gomez and H.J.M. Brady,200628157505759Craig, R.W.,200216444454Willis, S.N., L. Chen, G. Dewson, A. Wei and E. Naik et al.,2005L, but not Bcl-2, until displaced by BH3-only proteins.]]>1912941305Chen, L., S.N. Willis, A. Wei, B.J. Smith and J.I. Fletcher et al.,200517393403Han, J., L.A. Goldstein, B.R. Gastman and H. Rabinowich,20062811015310163Gomez-Bougie, P., R. Bataille and M. Amiot,20043431563164