International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2019.790.800WangTingting LiShan ZhaoLi GuoYunliang 72019157Background and Objective: Acute cerebral ischemia was caused by cerebral artery blockage, which was the most common type of cerebral ischemia and the second leading fatal disease. The aim of the current study was to analyze the effects of picroside II on brain tissues in rats following middle cerebral artery occlusion/reperfusion (MCAO/R). Materials and Methods: Healthy adult male Wistar rats were used to establish MCAO/R models by intraluminal thread methods. The experimental rats were randomly divided into sham, model, picroside II (Picr) and PET-α (Pifithrin-α hydrobromide, an inhibitor of p53) groups. The neurological deficit and infarct volume were assessed using mNSS test and TTC staining. The morphology and structure of cortical brain tissues were observed by H and E and transmission electron microscopy (TEM). Apoptosis was counted by TUNEL. Mitochondrial permeability transition pore (mPTP) was determined using spectrophotometer. The expressions of p-p53, Bcl-2, Bax, Cyt c and Caspase-3 were tested using WB. Results: Picroside II ameliorated the neurological dysfunction of MCAO/R rats, reduced the cerebral infarct volume and apoptosis accompanied by mPTP closing and the inhibition of p53 signaling pathway and PET-α also simulated the therapeutic effect by inhibiting p53 signaling pathway. Conclusion: It was concluded that the MCAO/R could activate the opening of mPTP and neuronal apoptosis by p53 signaling pathway in rats. Picroside II played a neuroprotective role in inhibiting the activation of p53 signaling pathway.]]>Moskowitz, M.A., E.H. Lo and C. Iadecola,201067181198Ji, Y., L. Teng, R. Zhang, J. Sun and Y. Guo,20173621324Jackman, K. and C. Iadecola,201522149160Nielsen, M.M., K.L. Lambertsen, B.H. Clausen, M. Meyer and D.R. Bhandari et al.,20162016Laptenko, O. and C. Prives,200613951961Irminger-Finger, I., W.C. Leung, J. Li, M. Dubois-Dauphin and J. Harb et al.,2001812551266Vaseva, A.V., N.D. Marchenko, K. Ji, S.E. Tsirka, S. Holzmann and U.M. Moll,201214915361548Savica, R., T.G. Beach, J.G. Hentz, M.N. Sabbagh and G.E. Serrano et al.,20191397681Xie, Y.L., B. Zhang and L. Jing,201840828837Perez, M.J. and R.A. Quintanilla,201742617Zhan, M., C. Brooks, F. Liu, L. Sun and Z. Dong,201383568581Martinou, J.C., S. Desagher and B. Antonsson,2000c release from mitochondria: All or nothing.]]>2E41E43Zhang, H., L. Zhai, T. Wang, S. Li and Y. Guo,201761267278Longa, E.Z., P.R. Weinstein, S. Carlson and R. Cummins,1989208491Brancho, D., N. Tanaka, A. Jaeschke, J.J. Ventura and N. Kelkar et al.,2003in vivo.]]>1719691978Kuang, S.Q., O. Medina-Martinez, D.C. Guo, L. Gong and E.S. Regalado et al.,2016FOXE3 mutations predispose to thoracic aortic aneurysms and dissections.]]>126948961Wang, T., L. Zhai, H. Zhang, L. Zhao and Y. Guo,201557335351Zhao, L., Y.L. Guo, X.J. Ji and M.Z. Zhang,20142014Zhao, L., T. Wang, Y. Zhou, X. Li, D. Wang and Y. Guo,2015115055Li, S., T. Wang, L. Zhai, K. Ge, J. Zhao, W. Cong and Y. Guo,201864144155Levine, A.J.,199788323331Milczarek, G.J., J. Martinez and G.T. Bowden,199760111Durukan, A. and T. Tatlisumak,200787179197Yan, J., M. Zheng and D. Zhang,2014105568Li, Q., Z. Li, X.Y. Xu, Y.L. Guo and F. Du,20101145804590Wu, N., W. Li, W. Shu and D. Jia,20148545554Marzo, I., C. Brenner, N. Zamzami, S.A. Susin and G. Beutner et al.,199818712611271Azarashvili, T., O. Krestinina, Y. Baburina, I. Odinokova and V. Akatov et al.,20162016Garrido, C., L. Galluzzi, M. Brunet, P.E. Puig, C. Didelot and G. Kroemer,2006c release from mitochondria.]]>1314231433Dagher, P.C.,200466506509Culmsee, C., X. Zhu, Q.S. Yu, S.L. Chan and S. Camandola et al.,200177220228Leker, R.R., M. Aharonowiz, N.H. Greig and H. Ovadia,2004187478486Khoo, K.H., K.K. Hoe, C.S. Verma and D.P. Lane,201413217236Chipuk, J.E., T. Kuwana, L. Bouchier-Hayes, N.M. Droin, D.D. Newmeyer, M. Schuler and D.R. Green,200430310101014Mihara, M., S. Erster, A. Zaika, O. Petrenko, T. Chittenden, P. Pancoska and U.M. Moll,200311577590