International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2019.560.566ZhaoZhongfang MaShaolin DongXianglin 52019155Background and Objective: Ginkgolide B (GB) is a terpene lactone isolated from a popular ancient Chinese tree Ginkgo biloba leaves which possess numerous biological activity. The current study was intended to examine the anti-melanogenic property of GB in B16F1 melanoma cell model by assessing cell viability, free radical production (ROS), tyrosinase activity and melanin content (synthesis). Materials and Methods: B16F1 murine melanoma cells were cultures in DMEM and pre-treated with GB and later stimulated with H2O2 or α-MSH to mimic UV induced hyperpigmentation (hyper-melanogenesis). Results: B16F1 murine melanoma cells (stimulated with H2O2 or α-MSH) and pretreated with increased concentration of GB (5,10, 20 and 50 μg mL1- 1 h before H2O2 or α-MSH stimulation) showed reduced (p<0.001) melanoma cell count (proliferation), lesser (p<0.001) ROS production, inhibit (p<0.01; p<0.001) tyrosinase activity and thus lowered (p<0.001) the melanin content via down regulating (p<0.001) the proteins expression of tyrosinase-related proteins-1 and 2 (TYRP-1 and 2), microphthalmia-associated transcription factor (MITF). Conclusion: This result clearly endowed that GB from Ginkgo biloba leaves showed better anti-melanogenic property in a dose-dependent fashion by abolishing excessive ROS generation (oxidative stress) as well as effectively inhibited tyrosinase activity and thereby suppress the production of melanin which reflects in decreased melanin count. Hence, it recommended that GB could be used as a skin whitening agent or derma-protective agent in the cosmetic industry.]]>Lajis, A.F.B., M. Hamid and A.B. Ariff,20122012Panich, U., K. Kongtaphan, T. Onkoksoong, K. Jaemsak and R. Phadungrakwittaya et al.,2010Alpinia galanga and Curcuma aromatica extracts correlates with their inhibition of UVA-induced melanogenesis.]]>26103116Yoon, W.J., M.J. Kim, H.B. Koh, W.J. Lee, N.H. Lee and C.G. Hyun,2010Stichopus japonicus) on melanogenic protein expression in murine B16 melanoma.]]>63742Chao, W.W., C.C. Su, H.Y. Peng and S.T. Chou,2017Melaleuca quinquenervia essential oil inhibits α-melanocyte-stimulating hormone-induced melanin production and oxidative stress in B16 melanoma cells.]]>34191201Ye, Y., G.X. Chou, H. Wang, J.H. Chu and Z.L. Yu,2010183235Eves, P.C., S. MacNeil and J.W. Haycock,200627444452Lee, S.Y., N. Baek and T.G. Nam,201631113Chang, T.S.,2012516611685Abdillahi, H.S., J.F. Finnie and J. Van Staden,2011Podocarpus species used in traditional medicine in South Africa.]]>136496503Kim, P.S., J.H. Shin, D.S. Jo, D.W. Shin and D.H. Choi et al.,2018Rhizoma arisaematis by increasing autophagy in B16F1 cells.]]>503309315Xia, S.H. and D.C. Fang,2007120922928Gill, I., S. Kaur, N. Kaur, M. Dhiman and A.K. Mantha,20171-42 induced oxidative damage and altered cellular responses in human neuroblastoma SH-SY5Y cells.]]>60S25S40Nabavi, S.M., S. Habtemariam, M. Daglia, N. Braidy, M.R. Loizzo, R. Tundis and S.F. Nabavi,20151522222232Pei, H.X., R. Hua, C.X. Guan and X. Fang,201596233239Feng, Z., X. Yang, L. Zhang, I.A. Ansari, M.S. Khan, S. Han and Y. Feng,20183224172427Zhi, Y., J. Pan, W. Shen, P. He and J. Zheng et al.,20163917871794Sun, L., Z. He, J. Ke, S. Li and X. Wu et al.,20158432440Bai, J. and A.I. Cederbaum,200327846604667Huang, Y.C., K.C. Liu, Y.L. Chiou, C.H. Yang, T.H. Chen, T.T. Li and L.L. Liu,2013205157164Rosenthal, M.H., J.W. Kreider and R. Shiman,1973569199Chan, W.H.,2007811771195Lou, C., H. Lu, Z. Ma, C. Liu and Y. Zhang,2019109563572Nichols, J.A. and S.K. Katiyar,20103027183Ma, W., J. Hu, Y. Cheng, J. Wang, X. Zhang and M. Xu,201575949959D'Mello, S.A.N., G.J. Finlay, B.C. Baguley and M.E. Askarian-Amiri,20162016Rougier, A., M. Schiller, T. Brzoska, M. Bohm, D. Metze, T.E. Scholzen and T.A. Luger,2004in vivo.]]>122468476Lee, J.H., B. Cho, H.J. Jun, W.D. Seo, D.W. Kim, K.J. Cho and S.J. Lee,201234805812Azam, M.S., M. Kwon, J. Choi and H.R. Kim,2018104582589