International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2019.509.5142O2-induced Cell Death and Interleukin-8 Production in Human Small Airway Epithelial Cells]]>SatoTakahiro HiroharaAyano IzumiYuki NakatomiTakayuki IwayamaKuninori OhtakiKo-Ichi HayaseNobumasa 42019154Background and Objective: The antibiotic minocycline (MINO) is known to have anti-inflammatory activity. This study aimed to examine the effects of MINO on hydrogen peroxide (H2O2)-induced cell death and interleukin (IL)-8 production, which have been shown to increase during inflammation, using small airway epithelial cells (SAECs). Materials and Methods: The SAECs were pretreated with MINO (1, 5 and 10 μM) for 24, 48 or 72 h and subsequently exposed to H2O2 (100 μM) for 6 h. Cell viability was assessed using the WST-8 assay and levels of IL-8 protein were measured using an enzyme-linked immunosorbent assay (ELISA). Tukey’s multiple comparison test was used for analysis of statistical significance. Results: Treatment of SAECs with 10 μM MINO or greater than 100 μM H2O2 significantly decreased cell viability. However, pretreatment with 1 μM MINO for 24 h significantly suppressed the H2O2-induced decrease in cell viability. In addition, IL-8 production increased in the H2O2 treated group as compared to the control group (not treated with H2O2), whereas IL-8 production decreased significantly following pretreatment with MINO (1, 5 or 10 μM) for 48 or 72 h. Conclusion: It was suggested that pretreatment of SAECs with low concentrations of MINO for 24 or 48 h has a cytoprotective effect and pretreatment for 48 or 72 h suppresses IL-8 production indicating a potential anti-inflammatory effect.]]>Khair, O.A., J.L. Devalia, M.M. Abdelaziz, R.J. Sapsford and R.J. Davies,1995814511457Pukhalsky, A.L., G.V. Shmarina, N.I. Kapranov, S.N. Kokarovtseva, D. Pukhalskaya and N.J. Kashirskaja,200413111117Garrido-Mesa, N., A. Zarzuelo and J. Galvez,2013169337352Garrido-Mesa, N., A. Zarzuelo and J. Galvez,2013671830Holguin, F.,201310150157Hou, Y., J. An, X.R. Hu, B.B. Sun and J. Lin et al.,20099120126Iwayama, K., A. Kusakabe, K. Ohtsu, T. Nawano and R. Tatsunami et al.,20172017Iwayama, K., J. Kimura, A. Mishima, A. Kusakabe, K.I. Ohtaki, Y. Tampo and N. Hayase,20182018Bajantri, B., S. Venkatram and G. Diaz-Fuentes,2018Mycoplasma pneumoniae: A potentially severe infection.]]>10535544Morimoto, N., M. Shimazawa, T. Amashima, H. Nagai and H. Hara,2005in vitro and in vivo ischemic neuronal damage.]]>1044815Jordan, J., F.J. Fernandez-Gomez, M. Ramos, I. Ikuta, N. Aguirre and M.F. Galindo,20074225231Parthasarathy, S., J. Barnett and L.G. Fong,19901044275283Reilly, C.A., J.L. Taylor, D.L. Lanza, B.A. Carr, D.J. Crouch and G.S. Yost,200373170181Kelly, R.G. and L.A. Kanegis,196711171183Naline, E., M. Sanceaume, L. Toty, H. Bakdach, M. Pays and C. Advenier,199132402404Pourgholami, M.H., A.H. Mekkawy, S. Badar and D.L Morris,2012125433440Fares, M., M. Abedi-Valugerdi, M. Hassan and Z. Potacova,2015463268274Test, S.T. and S.J. Weiss,19842O2 pool generated by human neutrophils.]]>259399405Rada, B. and T.L. Leto,2010Pseudomonas aeruginosa.]]>584917922Medzhitov, R.,2010140771776Chen, L., H. Deng, H. Cui, J. Fang and Z. Zuo et al.,2018972047218Song, X., A. Xu, W. Pan, B. Wallin and R. Kivlin et al.,20082O2-inducedcell death via JNK and p38 MAPK pathways.]]>22916Kraus, R.L., R. Pasieczny, K. Lariosa‐Willingham, M.S. Turner, A. Jiang and J.W. Trauger,200594819827Jabbour, H.N., K.J. Sales, R.D. Catalano and J.E. Norman,2009138903919Sun, J., H. Shigemi, Y. Tanaka, T. Yamauchi, T. Ueda and H. Iwasaki,20154397404Oliveira-Marques, V., H.S. Marinho, L. Cyrne and F. Antunes,20091122232243Martindale, J.L. and N.J. Holbrook,2002192115