International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2019.144.150GaoJiayu YangXiao HuJiangxia YangXiao 12019151Background and Objective: Though polysaccharides are considered to constitute the main medicinal properties of herbal Spina Gleditsiae, the current research on their isolation and the anticancer/antioxidant activities remain as an ongoing challenge. The purpose of this study was to develop an isolation technique for polysaccharides of Spina Gleditsiae (PSG) and then determined their anticancer and antioxidant properties. Materials and Methods: This study developed an ultrasonic-assisted method for PSG isolation and then the anticancer activities of isolated PSG were determined using MTT cytotoxic test. Moreover, the antioxidant effects of PSG were evaluated in the cell antioxidant, total antioxidant activity, ABTS free radical scavenging and Iron ion reduction assays. Results: In this study, the method of PSG isolation was firstly developed and characterized with acceptable yield rate (1.85±0.12%) and time/resources consuming, which greatly facilitate the current and future study of PSG. Meanwhile, the growth inhibitory and antioxidant effects of PSG were firstly determine in esophageal/liver cancer and human embryonic kidney cells in vitro. The isolated PSG were able to inhibit the growth of EC109 and HepG2 cancer cells with IC50 at 32.19±2.07 and 34.17±3.3 μg mL1, respectively. Moreover, PSG demonstrated the dose-dependent antioxidant, free radical scavenging and reductive activities. Conclusion: The PSG was confirmed as an active anticancer and antioxidant component of Spina Gleditsiae, providing a potential natural medicinal resource for further investigation and development. Moreover, the results of this study underpin the fundamental understanding of the medicinal activities of Spina Gleditsiae and support its current use in clinic of China.]]>State Pharmacopoeia Committee of the People's Republic of China,2010Gao, J., X. Yang and W. Yin,20162016Zhang, J.P., X.H. Tian, Y.X. Yang, Q.X. Liu and Q. Wang et al.,2016Gleditsia species: An ethnomedical, phytochemical and pharmacological review.]]>178155171Cao, R.R., J.Y. Gao, H.Q. Liu and W.P. Yin,201647707711Lee, J., J.M. Yi, H. Kim, Y.J. Lee, J.S. Park, O.S. Bang and N.S. Kim,2014Gleditsia sinensis thorns.]]>37612Liu, J., S. Willfor and C. Xu,201553161Zhao, Y.M., J. Wang, Z.G. Wu, J.M. Yang, W. Li and L.X. Shen,2016Lentinus edodes.]]>93136144Rostami, H. and S.M.T. Gharibzahedi,2017Malva sylvestris: Process optimization and potential functionalities.]]>101196206Wu, F., C. Zhou, D. Zhou, S. Ou and H. Huang,2017Hericium erinaceus and its signaling pathways involved in macrophage immunomodulatory activity.]]>37574585Gao, J., X. Yang, P. Liu and W. Yin,2016Scutellaria barbata and determination of their anticancer and antioxidant activities.]]>12754759Bhotmange, D.U., J.H. Wallenius, R.S. Singhal and S.S. Shamekh,2017Tuber aestivum.]]>1019Song, Y.R., S.K. Sung, E.J. Shin, C.W. Cho, C.J. Han and H.D. Hong,2018Aster scaber.]]>2018Lee, S.J., K. Park, S.D. Ha, W.J. Kim and S.K. Moon,2010Gleditsia sinensis thorn extract inhibits human colon cancer cells: The role of ERK1/2, G2/M-phase cell cycle arrest and p53 expression.]]>2418701876Lee, S.J., D.H. Ryu, L.C. Jang, S.C. Cho, W.J. Kim and S.K. Moon,2013Gleditsia sinensis thorns on human SNU-5 gastric cancer cells.]]>2916091616Ryu, S., K.M. Park and S.H. Lee,2016Gleditsia sinensis thorn attenuates the collagen-based migration of PC3 prostate cancer cells through the suppression of α2β1 integrin expression.]]>2016Yu, J.Q., Y.X. Xian, Y.L. Geng, D.J. Wang, H.L. Zhou and X. Wang,2015Gleditsia sinensis.]]>13343347Yi, J.M., J.S. Park, S.M. Oh, J. Lee and J. Kim et al.,2012Gleditsia sinensis thorn suppresses angiogenesis in vitro and in vivo.]]>2012Yi, J.M., J. Kim, J.S. Park, J. Lee and Y.J. Lee et al.,2015In vivo anti-tumor effects of the ethanol extract of Gleditsia sinensis thorns and its active constituent, cytochalasin H.]]>38909912Yin, W.P., H.Q. Liu, J.Y. Gao and R.R. Cao,2016Gleditsia sinensis and their cytotoxic activity to tumor cells.]]>1424242427Maynard, S., S.H. Schurman, C. Harboe, N.C. de Souza-Pinto and V.A. Bohr,200830210