International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2019.110.115QuanGuangqian ChenWeiwei WenYongtai YanYunpeng GuMali PanYaozhen 12019151Background and Objective: Salvianolic acid B (SB) is one of the active components of a popular Chinese herb called Salvia miltiorrhiza, which possess numerous biological activities including anti-tumor. Current study was framed to evaluate the chemoprotective efficiacy of SB on human breast cancer MCF-7 cells. Materials and Methods: MCF-7 cells were exposed to dimethyl sulfoxide (DMSO-Control) as well as treated with an increased concentration of SB (25, 50, 100 and 200 μm) to explore the anti-proliferative (Cell viability), pro-oxidative and pro-apoptotic activities. Statistical analysis was carried out by student t-test using SPSS software. Results: MCF-7 cells treated with an increased concentration of SB after 24 and 48 h showed a sharp decline in the count of viable MCF-7cells and thus showcased its potent anti-proliferative and toxicity property. A pronounced increase in ROS generation was noted in MCF-7 cells co-cultured with SB in dose and time-dependent fashion. Likewise, DNA fragmentation and apoptotic markers like caspase 3 and 9 were also significantly increased upon treatment with SB in a dose-dependent manner to display its pro-oxidative and pro-apoptotic activities. Moreover, the protein expression of Bcl2 and Bax were notably downregulated and upregulated respectively after administration with increased concentration of SB than DMSO treated MCF-7 cells. Conclusion: Taking together, that increased concentration of SB could show better chemoprotective activity on human breast cancer MCF-7 cells by exhibiting anti-proliferative, pro-oxidative and pro-apoptotic activities in a dose-dependent manner.]]>Bortolotto, L.F.B., F.R. Barbosa, G. Silva, T.A. Bitencourt and R.O. Beleboni et al.,2017trans-chalcone and licochalcone A against breast cancer cells is due to apoptosis induction and cell cycle arrest.]]>85425433Fan, L., K. Strasser-Weippl, J.J. Li, J. St Louis and D.M. Finkelstein et al.,201415e279e289.Shapiro, C.L. and A. Recht,200134419972008Nowacki, L., P. Vigneron, L. Rotellini, H. Cazzola and F. Merlier et al.,2015Beta vulgaris L.) extract induces apoptosis and autophagic cell death in MCF-7 cells.]]>2919641973Sahib, H.B., Z. Ismail, N.H. Othman and A.M.S. Abdul-Majid,20095273276Su, C.Y., Q.L. Ming, K. Rahman, T. Han and L.P. Qin,2015Salvia miltiorrhiza: Traditional medicinal uses, chemistry and pharmacology.]]>13163182Chen, X., J. Guo, J. Bao, J. Lu and Y. Wang,2014Salvia miltiorrhiza Bunge (Danshen): A systematic review.]]>34768794Liu, Y. and H. Shen,201814866872Jing, Z., W. Fei, J. Zhou, L. Zhang and L. Chen et al.,201676150961519Chen, T., W. Liu, X. Chao, L. Zhang, Y. Qu, J. Huo and Z. Fei,201184163168Wang, Z.S., P. Luo, S.H. Dai, Z.B. Liu, X.R. Zheng and T. Chen,201333921928Zhao, Y., Y. Guo and X. Gu,20112011Guo, L., C.K. Lei and M. Shan,20083115121514van Meerloo, J., G.J.L. Kaspers and J. Cloos,20112011pp: 237-245pp: 237-245Shashi, B., S. Jaswant, R.J. Madhusudana, S.A. Kumar and Q.G. Nabi,2006Cedrus deodara induces apoptosis and early nitric oxide generation in human leukemia Molt-4 and HL-60 cells.]]>147288Silva, G., M. Marins, A.L. Fachin, S.H. Lee and S.J. Baek,2016trans-chalcone in osteosarcoma: Involvement of Sp1 and p53.]]>5514381448Hecht, F., C.F. Pessoa, L.B. Gentile, D. Rosenthal, D.P. Carvalho and R.S. Fortunato,20163742814291Poillet-Perez, L., G. Despouy, R. Delage-Mourroux and M. Boyer-Guittaut,20154184192Zeng, Z., H. Zhang, X. Wang, K. Liu, T. Li, S. Sun and H. Li,20181526792685Wang, L.Q., D.F. Wang, X.M. Li, J. Li and Y. Guan,20151413991404Dai, X., L. Xiang, T. Li and Z. Bai,2016712811294Haji, H.A., H. Sheibak, M. Khosravi and J. Asadi,201622730Li, H., X. Li, M. Bai, Y. Suo, G. Zhang and X. Cao,201581479314799