International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2018.936.944XieZhanxiong WenTing ZhangZhichao HuangKaiyuan WuTiesong LiuJianghong WangXiaokang 72018147Background and Objective: Aging is featured by many mechanisms of protective immunity exhibit defects. Bergapten (BG), is a furanocoumarin derived from herbal and citrus extracts can act as antioxidant and selective anticancer agents. However, its immune regulation properties have not been well documented. The current study aimed to investigate whether bergapten would attenuate immuno senescence and to explore its immuno modulatory effect on immune responses in D-galactose-induced aging Balb/C mice. Materials and Methods: In the beginning of the experiment, survival test was performed on infected aging Institute of Cancer Research (ICR) mice with bergapten treatment. Male Balb/C mice were separated into normal or D-galactose-induced aging model. Production of serum cytokines such as anti-oxidant enzyme and malondialdehyde (MDA) were determined. Then, the model group was separated into three subgroups that were treated with BG and normal diet for 14 days by oral gavage, respectively. Namely groups D-gal+BG (20, 100 mg kg1) groups and D-gal group. Flow cytometric analysis and enzyme activity assays were used in isolated splenocytes. Modulatory effects of bergapten on T cell proliferation and the production of IFN-γ and IL-4 were also investigated. Results: BG (20 or 100 mg kg1) group reverses body weight and spleen index in aging progress significantly. Further experimental results showed that BG (20 mg kg1) treatment not only promoted T cell proliferation but also up-regulated IFN-γ and IL-4cytokinesin aging mice. Moreover, BG (20 mg kg1) treatment enhanced Th and Tc responses, which may participate in the process of eliminating the virus in an old age. Conclusion: This in vivo study firstly shown that bergapten has immuno modulate effect against D-galactose induces aging Balb/C mice.]]>Kaviani, E., M. Rahmani, A. Kaeidi, A. Shamsizadeh, M. Allahtavakoli, N. Mozafari and I. Fatemi,20173345560McElhaney, J.E. and R.B. Effros,200921418424Shaw, A.C., S. Joshi, H. Greenwood, A. Panda and J.M. Lord,201022507513GarffâTavernier, L., V. Beziat, J. Decocq, V. Siguret and F. Gandjbakhch et al.,20109527535Doan, V.M., C. Chen, X. Lin, V.P. Nguyen and Z. Nong et al.,2015617121718Po, J.L.Z., E.M. Gardner, F. Anaraki, P.D. Katsikis and D.M. Murasko,200212311671181Powers, D.C.,19931993Liang, Z., Y. Zhao, L. Ruan, L. Zhu and K. Jin et al.,2017157228Tanew, A., B. Ortel, K. Rappersberger and H. Honigsmann,198818333338De Amicis, F., S. Aquila, C. Morelli, C. Guido and M. Santoro et al.,20152015Panno, M.L., F. Giordano, P. Rizza, M. Pellegrino and D. Zito et al.,2012136443455Budzynska, B., K. Skalicka-Wozniak, M. Kruk-Slomka, M. Wydrzynska-Kuzma and G. Biala,2016In vivo modulation of the behavioral effects of nicotine by the coumarins xanthotoxin, bergapten and umbelliferone.]]>23322892300Yu, J., L. Wang, R.L. Walzem, E.G. Miller, L.M. Pike and B.S. Patil,20055320092014Liu, W.X., F.L. Jia, Y.Y. He and B.X. Zhang,20121821972202Bose, S.K., S. Dewanjee, R. Sahu and S.P. Dey,2011Heracleum nepalense root on production of proinflammatory cytokines.]]>2514441449Zhou, Y., J. Wang, W. Yang, X. Qi, L. Lan, L. Luo and Z. Yin,201748159168Ho, S.C., J.H. Liu and R.Y. Wu,200341518Cai, Y., Y.F. Li, L.P. Tang, B. Tsoi and M. Chen et al.,20152015Ding, X., J. Lu, R. Yu, X. Wang and T. Wang et al.,20162016Di Benedetto, A., M. Mottolese, F. Sperati, C. Ercolani and L. Di Lauro et al.,201723222462252Martin, R., C. Cordova, J.A. San Roman, B. Gutierrez, V. Cachofeiro and M.L. Nieto,201472250262Rios, J.L.,2010128114Zheng, M., Y. Ge, H. Li, M. Yan, J. Zhou and Y. Zhang,201438627634Campisi, J.,201375685705Smithey, M.J., K.R. Renkema, B.D. Rudd and J. Nikolich-Zugich,2011+ T cells combine to decrease clearance of L. monocytogenes in old mice.]]>4113521362Pae, M. and D. Wu,2017411435Muid, K.A., H.C. Karakaya and A. Koc,2014444260263Fraga, C.G., P.I. Oteiza and M. Galleano,2014In vitro measurements and interpretation of total antioxidant capacity.]]>1840931934Poniedzialek, B., P. Rzymski, M. Piet, M. Gasecka and A. Stroinska et al.,2018191548554Moradali, M.F., H. Mostafavi, S. Ghods and G.A. Hedjaroude,20077701724Luz, C., D. Collaziol, T. Preissler, I.M. da Cruz, L. Glock and M.E. Bauer,200613160169Guo, X., S.E. Stroup and E.R. Houpt,2008Entamoeba histolytica infection in CBA mice owes to intestinal IL-4 production and inhibition of protective IFN-γ.]]>1139146Haynes, L. and A.C. Maue,200921414417Saeidi, A., M. Buggert, K.F. Che, Y.Y. Kong, V. Velu, M. Larsson and E.M. Shankar,2015298126133