International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2018.1169.1178YangMing TengGaopeng LiGuangjian HuangTao XuRenguo 82018148Background and Objective: Inflammation is a key factor in the pathogenesis of arthritis. In the present study, investigated the potential of osthole, a major natural product, to treat monosodium urate crystal (MSU) induced acute gouty arthritis in rats and explore the mechanisms underlying osthole mediated immunomodulation. Materials and Methods: Rats were oral administrated with osthole at 24 h prior to 3 mg MSU injection, the volume and width of swelling ankle were recorded to evaluate the protection of osthole in vivo. LPS+MSU in Raw 264.7 cells was utilized to assess the effects of osthole on NLRP3 inflammasome in vitro. Results: Osthole could alleviate MSU-induced arthritis through inhibiting the generation of inflammatory factors and NLRP3 inflammasome activation in vivo. Pretreatment with osthole significantly suppressed MSU or LPS induced NF-κB signal along with the transcription of inflammatory factors. In addition, osthole decreased MSU induced oxidative stress and lysosomal damage. Current findings illustrated that osthole significantly suppressed NLRP3 inflammasome in synovial tissue and macrophage cells. The potential mechanism may be based on the attenuation of NF-κB mediated first signal and oxidative stress and lysosome mediated second signal in NLRP3 inflammasome activation. Conclusion: Osthole might be a promising therapeutic agent for alleviating gouty arthritis through inhibiting NLRP3 inflammasome.]]>So, A.K. and F. Martinon,201713639647Martinon, F., V. Petrilli, A. Mayor, A. Tardivel and J. Tschopp,2006440237241Torres, R., L. Macdonald, S.D. Croll, J. Reinhardt and A. Dore et al.,20096816021608Reber, L.L., T. Marichal, J. Sokolove, P. Starkl and N. Gaudenzio et al.,20146628812891Amaral, F.A., V.V. Costa, L.D. Tavares, D. Sachs and F.M. Coelho et al.,201264474484Yan, Y.H., S.H. Li, H.Y. Li, Y. Lin and J.X. Yang,201642398405Zhang, J., J. Xue, H. Wang, Y. Zhang and M. Xie,201125638643Liu, P.Y., D.C. Chang, Y.S. Lo, Y.T. His and C.C. Lin et al.,201833446453Wu, C., Z. Sun, B. Guo, Y. Ye, X. Han, Y. Qin and S. Liu,201785848058493Li, Y., Y. Li, F. Shi, L. Wang, L. Li and D. Yang,2018818525533Chiang, C.Y., C.C. Lee, C.K. Fan, H.M. Huang, B.L. Chiang and Y.L. Lee,201714935947Huang, T. and Z. Dong,20171649154921Yu, C., P. Li, D. Qi, L. Wang and H.L. Qu et al.,2017847964813Li, Y.Q., J.Y. Wang, Z.Q. Qian, Y.L. Li, W.N. Li, Y. Gao and D.L. Yang,2017811232239Wang, X.L., X. Shang, Y. Cui, X. Zhao, Y. Zhang and M.L. Xie,201537185192Yang, S.M., Y.L. Chan, K.F. Hua, J.M. Chang and H.L. Chen et al.,201473260269Tsai, Y.F., H.P. Yu, P.J. Chung, Y.L. Leu, L.M. Kuo, C.Y. Chen and T.L. Hwang,201589387400Heneka, M.T., M.P. Kummer, A. Stutz, A. Delekate and S. Schwartz et al.,2013493674678Dhanasekar, C., S. Kalaiselvan and M. Rasool,20152015Latz, E., T.S. Xiao and A. Stutz,201313397411Shi, J., Y. Zhao, K. Wang, X. Shi and Y. Wang et al.,2015526660665Vigano, E., C.E. Diamond, R. Spreafico, A. Balachander, R.M. Sobota and A. Mortellaro,20152015Yan, Y., W. Jiang, L. Liu, X. Wang, C. Ding, Z. Tian and R. Zhou,20151606273Yan, Y., W. Jiang, T. Spinetti, A. Tardivel and R. Castillo et al.,20133811541163Youm, Y.H., K.Y. Nguyen, R.W. Grant, E.L. Goldberg and M. Bodogai et al.,201521263269Mao, K., S. Chen, M. Chen, Y. Ma and Y. Wang et al.,201323201212Yu, N., S. Liu, X. Yi, S. Zhang and Y. Ding,2015179344353Wen, H., D. Gris, Y. Lei, S. Jha and L. Zhang et al.,201112408415Duewell, P., H. Kono, K.J. Rayner, C.M. Sirois and G. Vladimer et al.,201046413571361Zhou, R., A.S. Yazdi, P. Menu and J. Tschopp,2011469221225Nakahira, K., J.A. Haspel, V.A. Rathinam, S.J. Lee and T. Dolinay et al.,201112222230Bauernfeind, F., E. Bartok, A. Rieger, L. Franchi, G. Nunez and V. Hornung,2011187613617Zamudio-Cuevas, Y., C. Hernandez-Diaz, C. Pineda, A.M. Reginato, J.F. Cerna-Cortes, L. Ventura-Rios and A. Lopez-Reyes,20153416671672Zamudio-Cuevas, Y., K. Martinez-Flores, J. Fernandez-Torres, Y.A. Loissell-Baltazar and D. Medina-Luna et al.,20162016Lamkanfi, M. and V.M. Dixit,201228137161Cassel, S.L., S.C. Eisenbarth, S.S. Iyer, J.J. Sadler and O.R. Colegio et al.,200810590359040Orlowski, G.M., J.D. Colbert, S. Sharma, M. Bogyo, S.A. Robertson and K.L. Rock,201519516851697Okada, M., A. Matsuzawa, A. Yoshimura and H. Ichijo,20142893292632936