International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2017.227.236Aspergillus terreus on Colon Adenocarcinoma Caco-2 Cells]]>DikmenMiris CanturkZerrin ArtaganOge OzturkNilgun 32017133Objective: This study aimed to investigate the antioxidant and anticancer effects of the secondary metabolites of halotolerant Aspergillus terreus-2 (hAt-2) on colon adenocarcinoma (Caco-2) cells. The hAt-2 was isolated from Lake Tuz in Turkey. Materials and Methods: Ethyl acetate extract was prepared from the isolate of hAt-2 culture medium. The free radical scavenging activity and total phenolic content were evaluated using 2,2-diphenyl-l-picrylhydrazyl and Folin-Ciocalteu assays. The phenolic acids of the extract were evaluated using the high-performance liquid chromatography method. The cytotoxic effect of the extract was evaluated using 4-[3-(4-iodo-phenyl)-2-(4-nitrophenyl)-2H-5 tetrazolio]-1,3-benzene disulfonate assay. Cell proliferation was evaluated using the real-time cell analysis system (RTCA-DP). Results: The major phenolic acid in the hAt-2 extract was protocatechuic acid. The hAt-2 extract exhibited significant antiproliferative and apoptotic effects on Caco-2 cells in a time and concentration-dependent manner. The IC50 value of the extract was detected as 1859 μg mL1 at 72 h using the RTCA-DP system. Conclusion: The present study suggested that the hAt-2 extract has antioxidant, antiproliferative and apoptotic properties to act as an anti-cancer agent on Caco-2 cells.]]>Siegel, R.L., K.D. Miller and A. Jemal,201565529Hassan, L.E.A., M.B.K. Ahmed, A.S. Abdul Majid, H.M. Baharetha, N.S. Muslim, Z.D. Nassar and A.M.S. Abdul Majid,20142014Blagosklonny, M.V.,200512592602Ferlay, J., I. Soerjomataram, R. Dikshit, S. Eser and C. Matherset al.,2015136E359E386Prata‐Sena, M., A.A. Ramos, S. Buttachon, B. Castro‐Carvalho and P. Marques et al.,2016Neosartorya siamensis in human cancer cells.]]>3018621871Giri, B., A. Gomes, A. Debnath, A. Saha and A.K. Biswas et al.,2006Bufo melanostictus, Schneider) skin extract on U937 and K562 cells.]]>48388400Crowell, J.A.,20054118891910Greenlee, H.,2012282944Aluise, C.D., R. Sultana, J. Tangpong, M. Vore, D.S. Clair, J.A. Moscow and D.A. Butterfield,2010678147156Nematbakhsh, M., F. Ashrafi, Z. Pezeshki, Z. Fatahi, F. Kianpoor, M.H. Sanei and A. Talebi,20121190193Saad, M., C. Tafani, D. Psimaras and D. Ricard,201426634641Basmadjian, C., Q. Zhao, E. Bentouhami, A. Djehal and C.G. Nebigil et al.,20142014Frisvad, J.C.,2014Aspergillus, Penicillium and talaromyces species.]]>2014Malpure, P.P., A.S. Shah and A.R. Juvekar,2006Aspergillus candidus MTCC 2202 broth filtrate.]]>44468473Sharma, O.P. and T.K. Bhat,200911312021205Sanchez-Moreno, C., J.A. Larrauri and F. Saura-Calixto,199876270276Aksoy, L., E. Kolay, Y. Agilonu, Z. Aslan and M. Kargioglu,2013Thermopsis turcica.]]>20235239Folin, O. and V. Ciocalteu,192727627650Singleton, V.L. and J.A. Rossi,196516144158Ozturk, N., M. Tuncel and N.B. Tuncel,200730587596Engur, S., M. Dikmen and Y. Ozturk,2016388797Ishiyama, M., H. Tominaga, M. Shiga, K. Sasamoto, Y. Ohkura and K. Ueno,1996in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet.]]>1915181520Atienzar, F.A., K. Tilmant, H.H. Gerets, G. Toussaint and S. Speeckaert et al.,201116575587Bird, C. and S. Kirstein,20092009Solly, K., X. Wang, X. Xu, B. Strulovici and W. Zheng,20042363372Urcan, E., U. Haertel, M. Styllou, R. Hickel, H. Scherthan and F.X. Reichl,2010265158Limame, R., A. Wouters, B. Pauwels, E. Fransen and M. Peeters et al.,20122012Casciola-Rosen, L., A. Rosen, M. Petri and M. Schlissel,19969316241629Van Engeland, M., F.C.S. Ramaekers, B. Schutte and C.P.M. Reutelingsperger,199624131139Vermes, I., C. Haanen, H. Steffens-Nakken and C. Reutelingsperger,19951843951Dikmen, M., Z. Canturk, Y. Ozturk and Y. Tunali,2010Cytometry.]]>25749755Soobrattee, M.A., V.S. Neergheen, A. Luximon-Ramma, O.I. Aruoma and T. Bahorun,2005579200213Baba, S.A. and S.A. Malik,2015Arisaema jacquemontii blume.]]>9449454Frisvad, J.C., B. Andersen and U. Thrane,2008112231240Mohamed, H.F.,2012Fusarium solani and Emericella nidulans isolated from Wady El-Natron soil in Egypt against Caco-2 (ATCC) cell line.]]>2863869Saeed, N., M.R. Khan and M. Shabbir,2012Torilis leptophylla L.]]>2012Halliwell, B.,2007401111Ferguson, L.R.,2010690311Lorenzen, K. and T. Anke,19982329364Muller, K.,200156916