International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2016.655.662Tetrapleura tetraptera Fruit Extract on Ehrlich Ascites Tumor Cells]]>OzaslanM. KaragozI.D. LawalR.A. KilicI.H. CakirA. OdesanmiO.S. GulerI. EbuehiO.A.T. 62016126Background:Tetrapleura tetraptera fruit is widely used in Southern Nigeria for a range of pharmacological actions.
Methodology/Principal Findings: In this study, the cytotoxic and antiproliferative effects of the ethanolic extract of fruit were studied
in vitro and in vivo by using ehrlich ascites carcinoma cells and tumor modelling, respectively. Cytotoxic activity was determined by
incubating the ehrlich ascites carcinoma cells with 0.1, 1, 10 and 100 μg mL1 of the extract. In vivo study, 20, 40 and 80 mg kg1 b.wt.,
doses of the extract was inoculated by intraperitoneal administration to mice following to carcinoma cells inoculation by same way. The
extract was cytotoxic to carcinoma cells as assessed by both in vitro and in vivo experiments. Conclusion/Significance: The IC50 of the
extract was found to be 250 μg mL1. Results showed that the Tetrapleura tetraptera increased the life span of mice via reducing the
number of the viable EAC cells, decreasing in ascites fluid volume and tumor burden. The DNA fragmentation assay also showed that it
has a possible pro-apoptotic effect.]]>Pezzuto, J.M.,199753121133Reddy, L., B. Odhav and K.D. Bhoola,200399113Gruss, I., O. Handzel, S. Ingber and M. Beiser,20121512428, (In Hebrew)Chiorean, E.G., R. Ramasubbaiah, M. Yu, J. Picus and J.A. Bufill et al.,201217e13e23Fabbrocini, G., N. Cameli, M.C. Romano, M. Mariano, L. Panariello, D. Bianca and G. Monfrecola,20122012Essien, E.U., B.C. Izunwane, C.Y. Aremu and O.U. Eka,1994Tetrapleura tetraptera.]]>454751Aladesanmi, A.J.,2007Tetrapleura tetraptera: Molluscicidal activity and chemical constituents.]]>42336Nwaiwu, J.I. and P.A. Akah,1986Tetrapleura tetraptera.]]>18103107Ojewole, J.A.O.,2005Tetrapleura tetraptera (Taub) (Fabaceae) fruit aqueous extract in mice.]]>1910231029Ojewole, J.A.O. and C.O. Adewunmi,2004Tetrapleura tetraptera (Taub) [fabaceae] fruit aqueous extract in rats.]]>95177182Adewunmi, C.O.,1991Tetrapleura tetraptera, for schistosomiasis control in Nigeria.]]>1022133Ojewole, J.A.O. and S.K. Adesina,1983Tetrapleura tetraptera.]]>4999102Okokon, J.E., A.E. Udokpoh and B.S. Antia,2007Tetrapleura tetraptera fruit.]]>111537540Noamesi, B.K., J.F. Mensah, M. Bogale, E. Dagney and J. Adotey,1994421318Adesina, S.K. and J.A. Reisch,1985Tetrapleura tetraptera fruit.]]>2430033006Adewunmi, C.O., P. Furu and H. Madsen,1989Tetrapleura tetraptera Taub. (Mimosaceae) on the growth and egg production of Biomphalaria glabrata say and Lymnaea columella say.]]>38184Adewunmi, C.O., G. Dorfler and W. Becker,1990Biomphalaria glabrata and uptake of calcium.]]>53956959Maillard, M., C.O. Adewunmi and K. Hostettmann,1992Tetrapleura tetraptera.]]>3113211323Marston, A., M. Maillard and K. Hostettmann,199338209214Ngassapa, O., C.W.W. Beecher, J.M. Pezzuto, N.R. Farnsworth, T.O. Henderson and G.L. Boye,1993Tetrapleura tetraptera and evaluation of the mutagenic potential of molluscicidal extracts and isolates.]]>5618721877Aderibigbe, A.O., E.O. Iwalewa, S.K. Adesina, O.E. Ukponmwan and A.O. Adebanjo,2007Tetrapleura tetraptera fruit.]]>19177181Aderibigbe, A.O., E.O. Iwalewa, S.K. Adesina and O.I. Agboola,2010Tetrapleura tetraptera in mice.]]>6480486Note, O.P., A.C. Mitaine-Offer, T. Miyamoto, T. Paululat, D.E. Pegnyemb and M.A. Lacaille-Dubois,2008Tetrapleura tetraptera.]]>74PB142PB142Adewunmi, C.O. and V.O. Marquis,1987Tetrapleura tetraptera), Lapalapa pupa (Jatropha gossypyfolia), Endod (Phytolacca dodecandra) and Bayluscide.]]>16972Arun, T., B. Senthilkumar, K. Purushothaman and A. Aarthy,2012Phyllanthus amarus (L.) and its antibacterial activity.]]>547674771Wagner, H. and S. Bladt,19962nd Edn.,Pages: 384Pages: 384Justo, G.Z., N. Duran and M.L.S. Queiroz,2000Aspergillus oryzae.]]>388219226Ozaslan, M., I.D. Karagoz, M.E. Kalender, I.H. Kilic, I. Sari and A. Karagoz,2007In vivo antitumoral effect of Plantago major L. extract on Balb/C mouse with Ehrlich ascites tumor.]]>35841851Ozaslan, M., I.D. Karagoz, I.H. Kilic, B. Cengiz and M.E. Kalender et al.,2009Plantago major sap on Ehrlich ascites tumours in mice.]]>8955959Ozaslan, M., M.E. Zumrutdal, K. Daglioglu, I.H. Kilic and I.D. Karagoz et al.,2009L. inermis in mice with EAC.]]>5263267Isler, D, M. Ozaslan, I.D. Karagoz, I.H. Kilic and M. Karakok et al.,201466114120Muthuraman, M.S., S. Dorairaj, P. Rangarajan and B. Pemaiah,2008Tragia plukenetii R.Smith on Ehrlich ascites carcinoma in mice.]]>735273530Saluja, M.S., B. Sangameswaran, I.S. Hura, A. Sharma, S.K. Gupta and M. Chaturvedi,2011In vitro cytotoxic activity of leaves of Madhuca longifolia against Ehrlich Ascites Carcinoma (EAC) cell lines.]]>15557Mazumdar, U.K., M. Gupta, M. Maiti and M. Mukherjee,1997Hygrophila spinosa on Ehrlich ascites carcinoma and sarcoma-180 induced mice.]]>35473477Gupta, M., U.K. Mazumder, N. Rath and D.K. Mukhopadhyay,2000Cassia fistula L. seed against Ehrlich Ascites Carcinoma.]]>72151156Gupta, M., U.K. Mazumder, R.S. Kumar, T. Sivakumar and M.L.M. Vamsi,2004Caesalpinia bonducella against ehrlich ascites carcinoma in Swiss albino mice.]]>94177184Bromberg, N., J.L. Dreyfuss, C.V. Regatieri, M.V. Palladino and N. Duran et al.,20101864352Kavimani, S. and K.T. Manisenthlkumar,2000Enicostemma littorale on Dalton's ascitic lymphoma.]]>71349352Ueda, J.Y., Y. Tezuka, A.H. Banskota, Q. Le Tran and Q.K. Tran et al.,200225753760Hoagland, H.C.,1982995102Andreani, A., G. Scapini, I. Galatulas and R. Bossa,198372814815Fecchio, D., P. Sirois, M. Russo and S. Jancar,199014125132Adewunmi, C.O. and P. Furu,1989Tetrapleura tetraptera fruit, on Schistosoma mansoni and S. Bovis.]]>27277283Aderibigbe, A.O., E.O. Iwalewa, S.K. Adesina, A.O. Adebanjo and O.E. Ukponmwan,2007Tetrapleura tetrapetra fruit in mice. ]]>715201524Obidoa, O. and S.C. Obasi,199141283289