International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2015.67.7112]]>MasadehMajed M. AlzoubiKarem H. Al-azzamSayer I. 12015111Fluoroquinolones antibiotics action involves interfering with bacterial DNA
replication and transcription leading to increased oxidative stress and bacterial
cell death. Vitamin B12, on the other hand, has been shown to possess
DNA protective and antioxidant properties. In this study, the possible interactive
effect of Vitamin B12 on fluoroquinolones antibiotics-induced cytotoxicity
against several reference bacteria was investigated. Standard bacterial strains
included E. coli ATTC 35218, Staphylococcus aureus ATTC29213,
Pseudomonas aeruginosa ATTC 9027, S. epidermidis ATTC 12228,
A. baumannii ATTC 17978, P. mirabilis ATTC 12459 and Klebsiella
pneumoniae ATTC 13883. The antibacterial activity of fluoroquinolones (ciprofloxacin
and levofloxacin), with or without pretreatment of bacterial cells by vitamin
B12 was assessed using the disc diffusion method and by measuring
the Minimum Inhibitory Concentration (MIC) and zones of inhibition of bacterial
growth. All of the tested bacterial strains were sensitive to both ciprofloxacin
and levofloxacin. When pretreated with Vitamin B12, all bacterial
strains showed significantly smaller zones of inhibition and larger MIC values
compared ciprofloxacin or levofloxacin alone. In conclusion, results indicate
the possible antagonistic properties for Vitamins B12 when it is
used concurrently with flouroquinolones.]]>Al-Maskari, M.Y., M.I. Waly, A. Ali, Y.S. Al-Shuaibi and A. Ouhtit,201212 deficiency and hyperhomocysteinemia promote oxidative stress in adult type 2 diabetes.]]>28e23e26Al-Soud, Y.A. and N.A. Al-Masoudi,200314790796Albesa, I., M.C. Becerra, P.C. Battan and P.L. Paez,2004317605609Alzoubi, K., O.F. Khabour, N. Hussain, S. Al-Azzam and N. Mhaidat,201212 effects on DNA damage induced by pioglitazone.]]>7484851Alzoubi, K., O. Khabour, M. Khader, N. Mhaidat and S. Al-Azzam,201412 effects on DNA damage induced by paclitaxel.]]>37276280Becerra, M.C. and I. Albesa,2002Staphylococcus aureus.]]>29710031007Birch, C.S., N.E. Brasch, A. McCaddon and J.H. Williams,200912: Cobalamins are intracellular antioxidants in vitro.]]>47184188Campoli-Richards, D.M., J.P. Monk, A. Price, P. Benfield, P.A. Todd and A. Ward,198835373447Chen, C.R., M. Malik, M. Snyder and K. Drlica,1996258627637CLSI.,20098th Edn.,Pages: 65Pages: 65Drlica, K. and X. Zhao,199761377392Forsgren, A., A. Bredberg, A.B. Pardee, S.F. Schlossman and T.F. Tedder,198731774779Furqan, S. and S.A.U. Paracha,201464399402Gellert, M.,198150879910Gootz, T.D., J.F. Barrett and J.A. Sutcliffe,199034812Goswami, M., S.H. Mangoli and N. Jawali,2006Escherichia coli.]]>50949954Gurbay, A. and F. Hincal,200427233242Joksic, I., A. Leskovac, S. Petrovic and G. Joksic,200612 reduces ribavirin-induced genotoxicity in phytohemaglutinin-stimulated human lymphocytes.]]>209347354Lawrence, J.W., D.C. Claire, V. Weissig and T.C. Rowe,19965011781188Lawrence, J.W., S. Darkin-Rattray, F. Xie, A.H. Neims and T.C. Rowe,199351165174Lucock, M.,200071121138Mallet, L. and A. Huang,200539150152Neil, E. and G. Marsh,199912 (cobalamin)-dependent enzymes.]]>34139154Masadeh, M.M., N.M. Mhaidat, K.H. Alzoubi, S.I. Al-Azzam and A.I. Shaweesh,201264457462McCaddon, A. and P.R. Hudson,2007539991000McCaddon, A., B. Regland, P. Hudson and G. Davies,200212 deficiency and Alzheimer disease.]]>5813951399Nordmann, P., A. Pechinot and A. Kazmierczak,198924355363Oliphant, C.M. and G.M. Green,200265455464Oomori, Y., T. Yasue, H. Aoyama, K. Hirai, S. Suzue and T. Yokota,1988229197Pouzaud, F., K. Bernard-Beaubois, M. Thevenin, J.M. Warnet, G. Hayem and P. Rat,2004In vitro discrimination of fluoroquinolones toxicity on tendon cells: Involvement of oxidative stress.]]>308394402Reynold, E.,200612, folic acid and the nervous system.]]>5949960Saito, M., T. Sasaki and H. Matsuoka,200912 promotes Cx40 and HCN4 gene expression at an early stage of cardiomyocyte differentiation.]]>585760Majumdar, S., S. Mukherjee, A. Maiti, S. Karmakar and A.S. Das et al.,200912 prevents short‐term arsenic trioxide‐induced systemic and mitochondrial dysfunction and DNA damage.]]>24377387Solovieva, M.E., V.V. Soloviev and V.S. Akatov,200712b increases the cytotoxicity of short-time exposure to ascorbic acid, inducing oxidative burst and iron-dependent DNA damage.]]>566206214Solovieva, M.E., V.V. Solovyev, A.A. Kudryavtsev, Y.A. Trizna and V.S. Akatov,200812b enhances the cytotoxicity of dithiothreitol.]]>4418461856Stover, P.J.,200412 in health and disease.]]>62S3S12Umezawa, N., K. Arakane, A. Ryu, S. Mashiko, M. Hirobe and T. Nagano,1997342275281