International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2015.644.658Streptomyces rochei NEAE-25]]>El-NaggarNoura El-Ahmady 72015117One hundred and thirty actinomycetes soil-isolates were screened for their uricase activity. The most promising isolate, strain NEAE-25, was selected and identified on the basis of morphological, cultural, physiological and biochemical properties, together with 16S rRNA sequence as Streptomyces rochei NEAE-25 and the sequencing product was deposited in the database of GenBank under accession number HQ889312. The optimization of different process parameters for uricase production by Streptomyces rochei NEAE-25 and its validation using Plackett-Burman experimental design and response surface methodology was carried out during the present study. Fifteen variables were screened using Plackett-Burman experimental design. The most significant positive independent variables affecting enzyme production (incubation time, medium volume and uric acid concentration) were further optimized by central composite design. The maximum uricase production by Streptomyces rochei NEAE-25 after central composite design was 47.49 U mL1 with a three-fold increase as compared to the unoptimized medium (16.1 U mL1).]]>Abdel-Fattah, M.G. and N.A. Abo-Hamed,200249445454Adamek, V., B. Kralova, M. Suchova, O. Valentova and K. Demnerova,1989497268275Aghaie-Khouzani, M., H. Forootanfar, M. Moshfegh, M.R. Khoshayand and M.A. Faramarzi,2012Paraconiothyrium variabile.]]>60915Akhnazarova, S. and V. Kafarov,1982Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman,19972533893402Arslan, F.,2008854925500Azab, E.A., M.M. Ali and M.F. Fareed,200574454Bandaru, V.V.R., S.R. Somalanka, D.R. Mendu, N.R. Madicherla and A. Chityala,2006Zymomonas mobilis using response surface methodology.]]>38209214Banik, R.M., A. Santhiagu and S.N. Upadhyay,2007Sphingomonas paucimobilis ATCC-31461 in molasses based medium using response surface methodology.]]>98792797Box, G.E.P., W.G. Hunter and J.S. Hunter,1978pp: 291-334pp: 291-334Brogard, J.M., D. Coumaros, J. Frankckhauser, A. Stahl and J. Stahl,197217890895Capasso, G., P. Jaeger, W.G. Robertson and R.J. Unwin,20051141534159Chen, X.C., J.X. Bai, J.M. Cao, Z.J. Li and J. Xiong et al.,2009Microbacterium sp. no. 205 using response surface methodology.]]>100919924Choi, Y.J., E.J. Kim, Z. Piao, Y.C. Yun and Y.C. Shin,2004Bacillus sp. strain KCTC 0377BP and its application for the production of chitosan oligosaccharides.]]>7045224531Colloc'h, N., M. El Hajji, B. Bachet, G. L'Hermite and M. Schlitz et al.,19974947952Deepak, V., K. Kalishwaralal, S. Ramkumarpandian, S. Venkatesh Babu, S.R. Senthilkumar and G. Sangiliyandi,2008Bacillus subtilis using response surface methodology.]]>9981708174Demain, A.L. and S. Sanchez,200962516El-Naggar, N.E. and N.A.M. Abdelwahed,2012Streptomyces sp. strain NEAE-D.]]>1111851196El-Naggar, N.E. and N.A.M. Abdelwahed,2014Streptomyces viridochromogenes.]]>525363El-Naggar, N.E., N.A.M. Abdelwahed and O.M.M. Darwesh,2014Streptomyces aegyptia NEAE 102 as eco-friendly nanofactory.]]>24453464Gochman, N. and M.J. Schmitz,19711711541159Gulati, R., R.K. Saxena and R. Gupta,1997242326Ishikawa, J., A. Yamashita, Y. Mikami, Y. Hoshino and H. Kurita et al.,2004Nocardia farcinica IFM 10152.]]>1011492514930Kaushik, R., S. Saran, J. Isar and R.K. Saxena,2006Aspergillus carneus.]]>40121126Kelly, W.N. and T.D. Pelella,19871987pp: 1623-1632pp: 1623-1632Krishnan, S., S.G. Prapulla, D. Rajalakshmi, M.C. Misra and N.G. Karanth,1998196165Larsen, G. and M. Loghman-Adham,199618191194Suzuki, M.,1981Corynebacterium sp. U-96.]]>89599607Masseoud, D., K. Rott, R. Liu-Bryan and C. Agudelo,20051141174124Montgomery, D.C.,19913rd Edn.,Pages: 500Pages: 500Nakagawa, S., H. Oda and H. Anazawa,1995Escherichia coli strain expressing uricase.]]>5922632267Nakagawa, T., M. Mazzali, D.H. Kang, L.G. Sanchez-Lozada, J. Herrera-Acosta and R.J. Johnson,2006246770Nanda, P., P.E.J. Babu, J. Fernandes, P. Hazarika and R.R. Dhabre,2012Gliocladium viride.]]>33546Plackett, R.L. and J.P. Burman,194633305325Poovizh, T., P. Gajalakshmi and S. Jayalakshmi,2014Pseudomonas putida isolated from poultry waste.]]>23440Saitou, N. and M. Nei,19874406425Sambrook, J., E.F. Fritsch and T.A. Maniatis,19892nd Edn.,Pages: 397Pages: 397Shirling, E.B. and D. Gottlieb,1966Streptomyces species.]]>16313340Staneck, J.L. and G.N. Roberts,197428226231Stowe, R.A. and R.P. Mayer,1966583640Tamura, K., J. Dudley, M. Nei and S. Kumar,20072415961599Wang, Y.H., J.T. Feng, Q. Zhang and X. Zhang,2008Xenorhabdus nematophila with response surface methodology.]]>104735744Yazdi, M.T., G. Zarrini, E. Mohit, M.A. Faramarzi, N. Setayesh, N. Sedighi and F.A. Mohseni,2006Mucor hiemalis: A new source for uricase production.]]>22325330Kon, Y., Y. Dobashi and H. Katsura,1976Hyphomycetes isolated form the excrements of Cettia diphone cantans. II. The effectiveness of purines and pyrimidines as substrates (author's transl).]]>96277283Goodfellow, M., P. Kampfer, H.J. Busse, M.E. Trujillo, K.I. Suzuki, W. Ludwig and W.B. Whitman,20122nd Edn., Vol. 5, The Actinobacteria Part A.Wink, J.M.,20122012