International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2015.463.469Poria cocos]]>HangXiao XiaofeiWang BingyouYang DeqiangDou HaixueKuang 52015115The dried sclerotium of Poria cocos (Schw.) Wolf, referred to as Poria (Fuling in Chinese), was widely used as both food and traditional Chinese medicine. The present study aimed to investigate the immunoenhancing constituents of Poria, multi-mode separation methods that were applied to fraction splitting of Fuling to furnish Petroleum Ether Fraction (PEF), Ethyl Acetate Fraction (EAF), Water Eluated Fraction (WEF), Alcohol Eluated Fraction (AEF) and Crude Polysaccharide Fraction (CPF). The serum hemolysin against SRBC, the Clearance Rate of Carbon Particles, thymus index and Peripheral Blood Lymphocyte (PBL) transformation assays were used to explore the immunoenhancing fractions of Fuling. The minimal effective dose of Poria Water Decoction (WD) for its immunoenhancing action was examined to be 25 g kg-1 according to crude drug. Pharmacological experiment indicated that EAF and CPF were regarded as immunoenhancing fractions of Poria, further, the HPLC analysis indicated that triterpenoids are the main constituents of EAF. In addition, the triterpenoids and polysaccharides in different parts of P. cocos were compared, indicating the triterpenoids were increased in the order of Poria epidermis (Fuling-Pi)>red Poria (chi-Fuling)>white Poria (bai-Fuling) and the total saccharides and soluble saccharides of red Poria were higher than other parts. The polysaccharides and triterpenoids were the immunoenhancing fractions of Poria and the formere is stronger. The current results can also explain the application difference of the three parts of Poria.]]>Jang, T.R., M.F. Kao, C.H. Chen, K.C. Hsieh, W.Y. Lai and Y.Y. Chen,2011Poria cocos) in male collegiate wrestlers.]]>124530536Chen, Y.Y. and H.M. Chang,2004Poria cocos) on human leukemic U937 and HL-60 cells.]]>42759769Lin, Z., Y.B. Xu, X.K. Ran and D.Q. Dou,2013Poria cocos].]]>3843404346, (In Chinese)Li, B., Y.X. Ding, D.Q. Dou, X.K. Ran, Y.B. Xu, L.H. Li and T.G. Kang,2015Poria coco.]]>2015Liu, J., M. Gao, H. Yu, Y. Wang, K. Wu, H. Yuan and C. Zhu,200723291292Pei, R.J., L.H. Qi and X.J. Liu,199314238242Ding, Y., P. Chen, S. Xing and X.B. Ru,1994Epimedium polysaccharide against inhibitory action of azidothymidine on mouse hemopoietic and immune functions.]]>8203205Xu, S.Y.,1982Pages: 952Pages: 952Yang, W.D. and G.G. Li,2007Poria student prouduct and other different processing products.]]>7916918Chen, X.X., Y.J. Zhang, M.X. Li and Y. Wu,20103468Strauss, O., P.R. Dunbar, A. Bartlett and A. Phillips,201562458468Alipour, R., M. Adib, B. Hashemi-Beni and F. Sadeghi,20142014