International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2014.175.181XiaodanLi ChiNing ZhenZhou GuanxiWang GuangwenYang YunliangGuo 32014103The aim is to investigate the effects of Zhuang Jin Xu Gu
(ZJXG) decoction on the serum levels of Growth Hormone (GH), Calcitonin (CT)
and Parathyroid Hormone (PTH) and the femoral fracture healing in rats. Femur
fractures were generated in 72 male adult Wistar rats by cutting femur transversely
at the middle point. The ZJXG decoction was administered orally as drinking
water after surgery for 28 days. The healing process was analyzed by X-ray,
gross anatomy and Hematoxtlin Eosin (HE) staining. The serum levels of GH, CT
and PTH were assessed by enzyme linked immunosorbent assay (ELISA). X-ray, gross
anatomy and HE staining indicated that the fracture line of the femoral fracture-end
was clear at 7th day, fibrous callus tissue formed at 14th day, Calcium salt
deposited at 21th day and osseous callus tissues formed at 28th day. During
fracture (7-21 day), no difference of the callus tissue structure existed between
model and treatment groups, but in treatment group it was better than that in
model group at 28th day. ELISA results showed that the serum levels of GH, CT
and PTH were higher than those in the control group at 7-28 days (p<0.05).
There were no significant difference between model and treatment groups at 7-21
days, but in treatment group it was higher than that in model group at 28th
day (p<0.05). So, ZJXG decoction could promote the fracture healing by reducing
the decomposition of GH, CT and PTH and keeping their high levels in rats with
femur fracture.]]>Andreassen, T.T. and H. Oxlund,200373258264Arabi, A., P. Garnero, R. Porcher, C. Pelissier, C.L. Benhamou and C. Roux,20031817471752Chen, J.J., P.S. Yang, S.C. Pan, L.Z. Zhang and R.A. Dong et al.,199515860Gafni, R.I., J.S. Brahim, P. Andreopoulou, N. Bhattacharyya and M.H. Kelly et al.,20122718111820Garcia-Delgado, I., S. Prieto, L. Gil-Fraguas, E. Robles, J.J. Rufilanchas and F. Hawkins,199760155159Giardino, R., M. Fini, A.N. Nicoli, S. Gnudi, G. Biagini, M.G. Gandolfi and R. Mongiorgi,199788469477 (In Italian)Hedstrom, M., M. Saaf, E. Brosjo, C. Hurtig, K. Sjoberg, A. Wesslau and N. Dalen,200475394401Huang, P.J.,2012312021Huang, S., K. Dai, T. Tang and Y. Wang,2000384750Li, X.L., X.L. Luo, N.S. Yu and M.C. Liu,20039111113Lin, Y.P. and R.X. Zhou,1993657Liu, H.F., J.J.J. Chen, P.S. Yang, L.Z. Zhang and R.A. Dong et al.,19986711Liu, Z.H., S.R. Yang and C. Hui,2004206365Mehta, N.M., A. Malootian and J.P. Gilligan,2003926592676Mognetti, B., S. Marino, A. Barberis, A.S.B. Martin and Y. Bala et al.,201189163171Vestergaard, P. and L. Mosekilde,200415155159Munoz-Torres, M., G. Alonso and M.P. Raya,20043117132Oreffo, R.O.,20045419423Petersen, M.M., J.B. Lauritzen, P. Schwarz and B. Lund,199869347350Raschke, M., M.H. Rasmussen, S. Govender, D. Segal, M. Suntum and J.S. Christiansen,2007156341351Turhani, D., M. Weissenbock, E. Stein, F. Wanschitz and R. Ewers,200765485493Wallach, S., G. Rousseau, L. Martin and M. Azria,1999in vitro models of skeletal metabolism.]]>25509516Wang, X.J., Y.X. Pan, Z.X. Du, Z. Zhou, M. Liu and Y.L. Guo,20132824202422Guo, Y.L., X.J. Wang, Y.X. Pan, Z.X. Du and Z. Zhou,20122012Wang, Y.M., G.X. Pei and G. Wang,200143638Yang, D., J. Guo, P. Divieti and F.R. Bringhurst,200638485496Yang, D., R. Singh, P. Divieti, J. Guo, M.L. Bouxsein and F.R. Bringhurst,20074014531461Zheng, P.F., Z. Dong, J.G. Wang and Y. Lou,2009133538Zhou, Q.L. and L.J. Wang,199925586588