International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2013.157.163Carpinus turczaninowii]]>KoHa Na OhTae-Heon BaikJong Seok HyunChang-Gu KimSang Suk LeeNam Ho 2201392Development of natural products possessing anti-inflammatory
activities has been the focus of research in our laboratory. In this study,
the extract from Carpinus turczaninowii branches was studied and their
anti-inflammatory constituents were identified. The aqueous ethanol extract
was fractionated successively to afford n-hexane, ethyl acetate, n-butanol and
aqueous layers. Upon anti-inflammatory tests for Nitric Oxide (NO) production,
using RAW 264.7 cells, the ethyl acetate fraction exhibited potent inhibitory
activities without causing cell toxicities. The ethyl acetate fraction was subjected
to further purification to isolate carpinontriol A and carpinontriol B as active
constituents. These diarylheptanoid compounds were isolated for the first time
from this plant. The isolates reduced the lipopolysaccharide (LPS)-induced
secretion of NO and IL-6 productions in a dose-dependent manner in RAW 264.7
cells which indicates their anti-inflammatory effects. Based on these results,
it was suggested that C. turczaninowii extracts containing carpinontriols
A and B could be considered potential anti-inflammatory agents for pharmaceuticals
or cosmetics.]]>Chang, C.S. and K.S. Chang,2010Corylopsis coreana (Hamamelidaceae) and Carpinus laxiflora var. longispica (Betulaceae).]]>85270276Jachak, S.M.,20078411415Jeon, J.I. and J.S. Jang,2000Carpinus in eastern Asia primarily based on native taxa to Korea.]]>30139153Kang, S.H., J.E. Koo, H.J. Hong, V.B. Mathema and Y.S. Koh,2012Carpinus tschonoskii leaves extract in R848-stimulated bone marrow-derived macrophages and dendritic cells.]]>427782Lee, J.S., H.J. Kim, H.K. Parkand Y.S. Lee,2002Carpinus cordata.]]>6513671370Moon, J.Y., E.J. Yang, S.S. Kim, J.Y. Kang, G.O. Kim, N.H. Lee and C.G. Hyun,2011Sasa quelpaertensis phenylpropanoid derivative suppresses lipopolysaccharide-induced nitric oxide synthase and cyclo-oxygenase-2 expressions in RAW 264.7 cells.]]>131961967Yang, E.J., E.Y. Yim, G. Song, G.O. Kim and C.G. Hyun,20092245249Yang, E.J., J.Y. Moon, M.J. Kim, D.S. Kim and C.S. Kim et al.,201011315322Yoon, W.J., Y.M. Ham, S.S. Kim, B.S. Yoo and J.Y. Moon et al.,20093130143Yoon, W.J., N.H. Lee and C.G. Hyun,201059415421Yoon, W.J., J.Y. Moon, J.Y. Kang, G.O. Kim, N.H. Lee and C.G. Hyun,2010Neolitsea sericea essential oil attenuates LPS-induced inflammation in RAW 264.7 macrophages by suppressing NF-kappaB and MAPK activation.]]>513111316Yoon, W.J., J.Y. Moon, G. Song Y.K. Lee and M.S. Han et al.,2010Artemisia fukudo essential oil attenuates LPS-induced inflammation by suppressing NF-κB and MAPK activation in RAW 264.7 macrophages.]]>4812221229Zhang, R., K.A. Kang, M.J. Piao, J.W. Park and T.K. Shin et al.,2007Carpinus tschonosk against H2O2 induced oxidative stress.]]>13118122Zeilhofer, H.U. and K. Brune,200627467474