International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2012.46.52Desmodium gangeticum L.: An In-vitro Study]]>Mohamed ShabiM. UpadhyayL. 1201281The present study was planned to investigate the effect of Desmodium gangeticum (L.) (DG) on lysosomal hydrolases, phosphatases and electrolytes in mechanically induced myocardial ischemic injury in rats. The isolated hearts of rats were subjected to global no-flow ischemia, followed by reperfusion in a Langendorff isolated perfused heart model. The group first hearts (n = 6) served as control where as group second hearts (n = 6) were subjected 30 min of global no-flow ischemia followed by 20 min of reperfusion. The group third and fourth hearts (n = 12) were subjected for preconditioning with DG, at the doses of 50, 100 mg kg-1, before 30 min starting the coronary occlusion followed by 20 min reperfusion. At the end of the study, heart were excised and lysosomal hydrolases, membrane bound phosphatases activities and electrolytes levels were assessed in the cardiac tissues. The activities of different lysosomal enzymes and hydrolases were increased (p<0.05) significantly in the leakage as well as in heart tissue after ischemia-reperfusion injury. Whereas, in preconditioned heart with DG (50, 100 mg kg-1) significantly decreased activities of lysosomal hydrolases in the leakage as well as in the cardiac tissues were observed (p<0.05). Similarly, significant (p<0.05) decreased activity of membrane bound phosphatases such as Na+/K+ ATPase, Ca2+ and Mg2+ ATPase were also observed in the ischemic-reperfusion mediated injured hearts. It can be concluded that alteration in these enzyme activities may lead change in the electrolytes such as sodium, potassium and calcium content in the heart during ischemic reperfusion injury. However, DG preconditioned efficiently reversed the membrane-bound enzymes activity as well as maintained the normal electrolyte concentration.]]>Ballentine, R. and D.D. Burford,19571957pp: 1002-1035pp: 1002-1035Braunwald, E. and R.A. Kloner,19857617131719Bonting, S.L.,19701970pp: 257-263pp: 257-263Chernysheva, G.V., L.V. Stoida, G.G. Amaranrova and I.L. Kuzmina,1980++ transport and lipid peroxidation of mitochondrial and microsomal membranes of the heart.]]>89615618Chopra, R.N., S.L. Nayar and I.C. Chopra,19563rd Edn.,Pages: 329Pages: 329Conchie, J., A.L. Gelman and G.A. Levry,1967103609615Decker, R.S., A.R. Poole, E.E. Griffin, J.T. Dingle and K. Wildenthal,197759911921Decker, R.S. and K. Wildenthal,197838662673DeWood, M.A., J. Spores, R. Notske, L.T. Mouser, R. Burroughs, M.S. Golden and H.T. Lang,1980303897902Kurian, G.A., P. Sachu and T. Varghese,200597457461Ghosal, S. and S.K. Bhattacharya,1972D. gangeticum.]]>22434440Hjerten, S. and H. Pan,19832+-ATPase from erythrocyte membranes.]]>728281288Hearse, D.,19791979pp: 1-21pp: 1-21Hoffstein, S., D.E. Gennaro, G. Weissmann, J. Hirsch, F. Streuli and A.C. Fox,197579193206Liu, H.R., F. Gao, L. Tao, W.L. Yan and E. Gao et al.,2004142627634Jennings, R.B. and D.M. Yellon,19921992pp: 1-11pp: 1-11Kawai, Y. and K. Anno,1971Ommastrephes sloani pacificus I. Hyaluronidase.]]>242428436Ueda, K., H. Takano, H. Hasegawa, Y. Niitsuma, Y. Qin, M. Ohtsuka and I. Komuro,200626e108e113Kennett, F.F. and W.B. Weglicki,198743759768King, J.,19651965pp: 191-197pp: 191-197Kirtikar, K.R. and B.D. Basu,19872nd Edn.,pp: 756-760pp: 756-760Kirthikar, K.R. and B.D. Basu,1975pp: 758-760pp: 758-760Moens, AL., M.J. Claeys, J.P. Timmermans and C.J. Vrints,2005100179190Moore, J.C. and J.E. Morris,198219157159Mourelle, M. and M.T. Franco,19914 induced liver cirrhosis: Protection by silymarin.]]>4810831090Nirmala, C. and R. Puranakrishnan,1996514751Ohnishi, T., T. Suzuki, Y. Suzuki and K. Ozawa,19822+-ATPase activities in normal, regenerating and malignant cells.]]>6846774Padmanabhan, M. and P.S.M. Prince,200780972978Panagia, V., G.N. Pierce and K.S. Dhalla,198517411420Purushothaman, K.K., V.M. Kishore, V. Narayanaswami and J.D. Connolly,1971Desmodium gangeticum (Leguminosae).]]>197124202422Purushothaman, K.K., S. Chandrasekharan, K. Balakrishna and J.D. Connolly,1975Desmodium gangeticum.]]>1411291130Ravichandran, L.V., R. Puvanakrishnan and K.T. Joseph,199145615Ravichandran, L.V., R. Puvanakrishnan and T.K. Joseph,199022387396Riccutti, M.A.,197230492497Sreepriya, M., N. Saravanan, T. Devaki and M. Nayeem,1999271926Sapolsky, A.I., R.D. Altman and D.S. Howell,19733214891493Sudharsan, P.T., Y. Mythili, E. Selvakumar and P. Varalakshmi,20062822329Trump, B.F., I.K. Bereezeky, T. Sato, K.V. Liaho, P.C. Phelps and N. Declaris,198457281287Yogeeta, S.K., A. Gnanapragasam, S. Senthilkumar, R. Subhashini and T. Devaki,200680258263Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275