International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2012.373.381SumathiC. JayashreeS. SekaranG. 5201285The focal theme of the present investigation is to explore the antimicrobial activity of Tannery Fleshing (TF) utilizing Labeo rohita gut bacteria against common microbial pathogens. The study involves isolation of the bacteria through culture technique, biochemical characterization, Scanning Electron Microscopic and phylogenetic cluster analysis. Antimicrobial activities were determined through agar diffusion technique. Partial 16S-rRNA gene sequencing analysis shows that the isolate exhibited homology with Bacillus megaterium. Among the bacterial pathogens maximum inhibition was observed against Salmonella typhi and Bacillus subtilis when compared to standard antibiotic followed by Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. Minimum inhibitory effect was observed with Staphylococcus aureus and Pseudomonas aeruginosa. Most susceptible fungal pathogens include Microsporum canis and Aspergillus fumigatus whereas Trichophyton mentagrophytes and Penicillium chrysogenum were least susceptible. Antifungal activity of Bacillus megaterium remained almost similar to standard drug in Fusarium moniliforme, Aspergillus niger, Candida albicans. The in vitro studies indicate that Bacillus megaterium ANFLR1 isolated from Labeo rohita can inactivate pathogenic Gram-positive and Gram-negative bacteria as well as fungi and can be of potential use as an antibiotic and probiotic.]]>Abdelhamid, A.M., A.I. Mehrim, M.I. El-Barbary, S.M. Ibrahim and A.I. Abd El-Wahab,20092133145Motta, A.S., F. Cladera-Olivera and A. Brandelli,200435307310Andrews, J.M.,200148516Arasu, M.V., V. Duraipandiyan, P. Agastian and S. Ignacimuthu,2009In vitro antimicrobial activity of Streptomyces spp. ERI-3 isolated from Western Ghats rock soil (India).]]>192228Kumar, G., L. Karthik, K.V. Bhaskara Rao and N. Aravamuthan,2010In vitro antagonistic activity of soil actinobacteria against multi drug resistant bacteria.]]>2507516Aruna, S., K. Vijayalakshmi, M. Shashikanth, M.S. Rani and K. Jyothi,2008Streptomyces tritolerans (strain AS1) isolated from earthworm gut (Eisenia foetida) against plant pathogenic bacteria and fungi.]]>14655Aslim, B., N. Saglam and Y. Beyatli,2002Bacillus isolates from soil.]]>264148Bairagi, A., K.S. Ghosh, S.K. Sen and A.K. Ray,200210109121Bali, V., P.S. Panesar and M.B. Bera, 20111113119Bechard, J., K.C. Eastwell, P.L. Sholberg, G. Mazza and B. Shkura,1998Bacillus subtilis.]]>4653555361Betina, V.,198314334433450Chen, W., P.H. Cooke, G.L. DiMaio, M.M. Taylor and E.M. Browm200196262267Cowan, M.M.,199912564582D'Arienzo, R., F. Maurano, G. Mazzarella, D. Luongo, R. Stefanile, E. Ricca and M. Rossi,2006Bacillus subtilis spores reduce susceptibility to Citrobacter rodentium-mediated enteropathy in a mouse model.]]>157891897Deepika, L. and K. Kannabiran,2010Streptomyces sp. VITDDK3 isolated from Ennore coast, Tamil Nadu India.]]>2188196Dhayalan, K., N.N. Fathima, A. Gnanamani, J.R. Rao, B.U. Nair and T. Ramasami,200727760767Drabløs, F., D.G. Nicholson and M. Rønning, 19991431433442Pishva, E., N. Hassannia, M.R. Fazeli, A. Havaee and H. Jamalifar et al.,2009Lactobacillus strains isolated from traditional yogurts. ]]>811321137Gadaga, T.H., A.N. Mutukumira, J.A. Narvhus and S.B. Feresu,199953111Sugita, H., J. Kawasaki and Y. Deguchi,199724105108Sugita, H., Y. Hirose, N. Matsuo and Y. Deguchi,1998Bacillus sp. strain NM 12, an intestinal bacterium of Japanese coastal fish.]]>165269280Sumathi, C. and G. Sekaran,2011Bacillus megaterium, Serratia marcescens and novel Pontibacter species.]]>11134139Taylor, M.M., C.K. Liu, N. Latona, W.N. Marmer and E.M. Brown,200297225234Therese, K.L., A.R. Anand and H.N. Madhavan,19988210781082Tosato, V., A.M., Albertini, M. Zotti, S. Sonda and C.V. Bruschi,1997Bacillus subtilis. 168.]]>14334433450Trust, T.J. and R.A. Sparrow,19742012191228Turnbull, P.C.B.,1996Bacillus.]]>1996Utkhede, R.S.,1984Bacillus subtilis to Phytophthora cactorum.]]>6210321035Valero, M., L.A. Hernandez-Herrero and M.J. Giner,200724671677Vary, P.S.,199414010011013Vaseeharan, B. and P. Ramasamy,2003Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon.]]>368387NCCLS,2002NCCLS Document M38-A.Zinsser, H.,19981998pp: 128-160pp: 128-160Nakayama, T., H. Lu and N. Nomura,2009Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp.]]>49679684Nikaido, H. and M. Vaara,198549132Paik, S.H., A. Chakicherla and J.N. Hansen,1998Bacillus subtilis 168.]]>273134142Patterson, J.A. and K.M. Burkholder,200382627631Perez, C., C. Suarez and G.R. Castro,1992Bacillus subtilis MIR15.]]>26331336Perez, C., C. Suarez and G.R. Castro,1993Bacillus circulans cluster.]]>382528Peypoux, F., J.M. Bonmatin and J. Wallach,199951553563Pinchuk, I.V., P. Bressollier, B. Verneuil, B. Fenet, I.B. Sorokulova, F. Megraud and M.C. Urdaci,2001In vitro anti-Helicobacter pylori activity of the probiotic strain Bacillus subtilis 3 is due to secretion of antibiotics.]]>4531563161Pinotti, L.M., A.F. Silva, R.G. Silva and R.L. Giordano,2000Bacillus megaterium ATCC 14945.]]>84-86655663Pinotti, L.M., R.G. Silva, R.C. Giordano and R.L.C. Giordano,2002Bacillus megaterium ATCC 14945.]]>98679686Premalatha, M. and P. Dhasarathan,201143137Raja, A., P. Gajalakshmi, R.M. MohammedMahroop and M. Imran,20094201209Raja, A. and P. Prabakarana,201117584Ramin, M., A.R. Alimon, K. Sijam and N. Abdullah,20083565568Katsifas, E., A.E. Giannoutsou, M. Lambraki, M. Barla and A.D. Karagouni,2004315762Katz, E. and A.L. Demain,1987Bacillus: Chemistry, biogenesis and possible function.]]>41449474Kim, H.S, I.J. Park, S.W. Choi, K.H. Choi, G.P. Lee, S.J. Ban and C.H. Lee,200341196201Klein, C., C. Kaletta and K.D. Entian,1993Lantibiotic subtilin is regulated by histidine kinase/response regulator system.]]>59296303Kuipers, O.P., H.S. Rollema, W.M.G.J. Yap, H.J. Boot, R.J. Siezen and W.M. de Vos,199226723402346Kucharoenphaisan, K.S. and K. Sinma,201276873Milner, J.L., S.J. Raffel, B.J. Lethbridge and J. Handelsman,1995Bacillus cereus UW85.]]>43685691Morikawa, M., M. Ito and T. Imanaka,1992Bacillus pumilus A-1 and cloning and nucleotide sequence of the regulator gene, psf-1.]]>74255261Morita, M., K. Tomita, M. Ishizawa, K. Takagi, J. Kawamura, H. Takahashi and T. Morino,1999Bacillus megaterium strain NK84-0128.]]>63563566Ahmad, M., D.G. Samith and S. Mahmood,2002Enterococcus faecalisagainst selected isolatesand some pathogenic microorganisms.]]>2343345Galvez, A., M. Maqueda, M. Cordovilla, M. Martinez-Bueno, M. Lebbadi and E. Valdivia,19943813141319Kumar, A.G., N. Nagesh, T.G. Prabhakar and G. Sekaran,2008Synergistes sp. utilizing proteinaceous solid waste from tanneries.]]>9923642372Weinstein, R.A., R. Gaynes, J.R. Edwards and National Nosocomial Infections Surveillance System,200541848854Gibson, G.R. and M.B. Roberfroid,199512514011412Gutowski-Eckel, Z., C. Lein, K. Siegers, K. Bohm, M. Hammelmann and K.D. Etian,1994Lantibiotic subtilin.]]>60111Reddy, N.G., D.P.N. Ramakrishna and S.V.R. Gopal,2011Streptomyces rochei (MTCC 10109) showing antagonistic activity against selective human pathogenic microorganisms.]]>4114Stover, A.G. and A. Driks,1999Bacillus subtilis spore-associated protein.]]>18116641672Sharp, R.J., M.D. Scawen and T. Atkinson,1989Bacillus.]]>1989pp: 255-292pp: 255-292Silo-Suh, L.A., B.J. Lethbridge, S.J. Raffel, H. He, J. Clardy and J. Handelsman,19946020232030Sarker, D., N. Roy and T. Yeasmin,2010Bacillus thuringinesis strain from dump soil.]]>6127132Servin, A.L.,200428405440Gharaei-Fathabad, E., M.A. Tajick-Ghanbary and N. Shahrokhi,2009Penicillium species isolated from agricultural soils of Northern Iran.]]>2009