International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2011.821.828SharmaR.K. ChauhanP.K. FuliaA. 8201178Endosulphan is a xenoestrogen that imitates the effect of estrogens, causing reproductive and developmental abnormalities in mammals. The aim of the present investigation was to analyze the effect of vitamin E in rescue of degenerating changes induced by endosulphan in granulosa cells of goat Capra hircus in vitro. On the basis of colour and texture normal follicles (3-5 mm in diameter) were selected for the tissue culture. The follicles were divided into two groups (one control+two experimental groups). Experimental group (A) was exposed with 100 nmol mL-1 endosulphan concentration. Experimental group (B) was exposed with 100 nmol mL-1 endosulphan as well as supplemented with 100 μmol L-1 concentration of vitamin E (α-Tocopherol). Harvesting was carried out after 1 h, 4 h and 8 h of exposure. Control was run simultaneously along with all the experimental groups. Endosulphan at dose level 100 nmol mL-1 induced a decline in cell diameter from 7.5±0.0456 in control to 4.5±0.1024, 3.7±0.1001 and 3.2±0.1154 μm after exposure of 1, 4 and 8 h, respectively but in case of endosulphan supplemented with vitamin E, there was less decline in cell diameter that was 6.4±0.1235, 4.8±0.1809 and 4.1±0.0809 μm after exposure of 1, 4 and 8 h, respectively. Endosulphan induced atretogenic changes like hyalinization of granulosa cells, crinkled and wavy membranes and pycnosis and thus affects the functions in adult goat due to the oxidative stress. Vitamin E treatment at dose level 100 μmol L-1 in experimental group (B) these atretogenic changes were milder and restore the normal structure of granulosa cells.]]>Harvey, F.,2010165656McLachlan, J.A.,200122319341Zoeller, R.T., A.L.S. Dowling, C.T.A. Herzig, E.A. Iannacone, K.J. Gauger and R. Bansal,2002110355361Gray, L.E. Jr., V.S. Wilson, T. Stoker, C. Lambright and J. Furr et al.,20062996104Biswas, A.K., N. Kondaiah, A.S.R. Anjaneyulu and P.K. Mandal,201044655Zidan, N.E.H.A.,200955157Alahyary, P., M.I. Poor, F.F. Azarbaijani and V. Nejati,200811127130Masouleh, F.F., B.M. Amiri, A.R. Mirvaghefi and M.A. Nemtollahi,2011In vitro effects of diazinon on male reproductive tissue and sperm motility of caspian kutum (Rutilus frisii kutum).]]>5108116Gellert, R.J.,197816131138Bay, K., C. Asklund, N.E. Skakkebaek and A.M. Andersson,2006207790Skakkebaek, N.E., E.R. De Meyts and K.M. Main,200116972978Buttke, T.M. and P.A. Sandstrom,199415710Verhaegen, S., A.J. McGowan, A.R. Brophy, R.S. Fernandes and T.G. Cotter,19955010211029Gorman, A., A. McGowan and T.G. Cotter,19974042733Tilly, J.L. and K.I. Tilly, 1995136242252Fujii, J., Y. Iuchi and F. Okada,200534352Aitken, R.J. and M.A. Baker,20062506669Halliwell, B. and J.M.C. Gutteridge,20072nd Edn.,Saradha, B., S. Vaithinathan and P.P. Mathur,2008243116123Lucesoli, F. and C.G. Fraga,1999132179186Iribhogbe, O.I., A. Aigbiremolen, U. Akpamu, J.E. Emordi and E.O. Nwoke,201146270Varayoud, J., L. Monje, T. Bernhardt, M. Munoz-de-Toro, E.H. Luque and J.G. Ramos,200826138145Sharma, R.K. and A. Fulia,200910119126Sharma, R.K., A. Fulia and P.K. Chauhan,2010in vitro.]]>10624630Vale, C., E. Fonfria, J. Bujons, A. Messeguer, E. Rodriguez-Farre and C. Sunol,2003117397403 Sharma, R.K., P.K. Chauhan and A. Fulia,2010in vitro.]]>4214222Pearse, A.G.E.,1968Guney, M., H. Demirin, B. Oral, M. Ozguner, G. Bayhan and I. Altuntas,200726491498Fulia, A., P.K. Chauhan and R.K. Sharma,2011Capra hircus in vitro.]]>6133140Evans, H.M. and K.S. Bishop,192256650651Akmal, M., J.Q. Qadri, N.S. Al-Waili, S. Thangal, A. Haq and K.Y. Saloom,20069440442El-Demerdash, F.M., M.I. Yousef, F.S. Kedwanyand and H.H. Baghdadi,200493443459Keskes-Ammar, L., N. Feki-Chakroun, T. Rebai, Z. Sahnoun and H. Ghozzi et al.,2003498394Esterbauer, H., M. Dieber-Rotheneder, G. Striegl and G. Waeg,199153314S321SZar, J.H.,19844th Edn.,