International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2011.400.404Camellia sinensis]]>Deep KaurChanchal SarafSwarnlata 3201173The main antioxidant polyphenols present in Camellia sinensis (green tea) are known as epicatechins which function as anti-inflammatory or anticarcinogenic agents. There are various evidences in the literature for the protective effects that green tea polyphenols exert on the skins immune system on oral and topical application. The aim of this study was to produce green tea extract and to evaluate its photochemoprotective ability. The alcoholic extract of green tea leaves was prepared by continuous hot extraction method using Soxhlet apparatus. The antioxidant activity of the extract was assessed by reducing power estimation method in which the antioxidant activity of extract was compared with that of standard (ascorbic acid). The individual antioxidant activity obtained was 51.12±1.8% and combined antioxidant activity (ascorbic acid with extract) showed additive synergistic effect as compared to that of standard. In vitro SPF was determined according to the spectrophotometric method where alcoholic dilution of green tea extract was prepared and in vitro photoprotective activity was studied by UV spectrophotometeric method in the range of 290-320 nm. The extract produced high absorbances at 290-320 nm wavelength range and SPF obtained was 18.10±0.05 which collectively confirmed the photoprotective activity of green tea polyphenols. The higher antioxidant activity and sun protection factor of green tea extract could be utilized in the preparation of photoprotective cosmetic formulations which could prevent the skin with harmful effects of ultra violet radiations.]]>Chanchal, D. and S. Swarnlata,200927176Ashawat, M.S., A. Gupta, S. Shailendra and S. Swarnlata,20073191195Atrooz, O.M.,20091210631068Ashawat, M.S., S. Shailendra and S. Swarnlata,2007In vitro antioxidant activity of ethanolic extracts of Centella asiatica, Punica granatum, Glycyrrhiza glabra and Areca catechu.]]>11316Svobova, A., J. Psotova and D. Walterova,2003147137145McKay, D.L. and J.B. Blumberg,200221113USDA,20032003Afaq, F. and H. Mukhtar,200615678684Nihal, M., N. Ahmad, H. Mukhtar and G.S. Wood,2005114513521Tang, F.Y., N. Nguyen and M. Meydani,2003106871878Jung, Y.D. and L.M. Ellis,200182309316Qanungo, S., M. Das and S. Haldar and A. Basu,200526958967Ahmad, N., P. Cheng and H. Mukhtar,2000275328334Sato, D.,199969399Ashawat, M.S., S. Shailendra and S. Swarnlata,20072135141Aburjai, T. and F.M. Natsheh,2003179871000Bendova, H., J. Akrman, A. Krejci, L. Kuba and D. Jirova et al.,2007In vitro approaches to evaluation of sun protection factor.]]>2112681275Wood, C. and E. Murphy,20001673844Mansur, J.S., M.N.R. Breder, M.C.A. Mansur and R.D. Azulay,198661121124Santos, E.P., Z.M. Freitas, K.R. Souza and S. Garcia,1999In vitro and in vivo determinations of sun protection factors of sunscreen lotions with octylmethoxycinnamate.]]>2115Ashawat, M.S., S. Saraf and S. Saraf,2006Boerhavia diffusa.]]>3257260Sayre, R.M., P.P. Agin, G.J. Levee and E. Marlowe,1979in vivo and in vitro testing of sunscreening formulas.]]>29559566WHO,2004pp: 30pp: 30Rajpal, V.,2004Rajeshwar, Y., K.G.P. Senthil and M. Gupta, U.K. Mazumdar,2005in vitro antioxidant activities of methanol extracts of M. Prurines seeds.]]>123136Adesegun, S.A., N.A. Elechi and H.A. Coker,2008Sapium ellipticum.]]>11453457Vinson, J.A., X. Su, L. Zubik and P. Bose,20014953155321Meyers, K.J., C.B. Watkins, M.P. Pritts and R.H. Liu,20035168876892Kahkonen, M.P., A.I. Hopia, H.J. Vuorela, J.P. Rauha, K. Pihlaja, T.S. Kujala and M. Heinonen,19994739543962Zhu, N., M. Wang, G.J. Wei, J.K. Lin, C.S. Yang and C.T. Ho,200173345349Ashawat, M.S., S. Shailendra and S. Swarnlata,200842319322Khazaeli, P. and M. Mehrabani,2008759Kaur, C.D. and S. Saraf,2010In vitro sun protection factor determination of herbal oils used in cosmetics.]]>22225