International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2010.888.895Cinnomomum iners Standardized Leaves Methanolic Extract]]>MustaffaF. IndurkarJ. IsmailS. MordiM.N. RamanathanS. MansorS.M. 6201066This study investigated the antioxidant capacity and safety parameters (toxicity) of Cinnomomum iners standardized leaves methanolic extract (CSLE) to provide information regarding long term usage of this plant. CSLE exhibited high total phenolic (211.94±12.04 mg GAE g-1 plant material) and flavonoid (13.38±0.08 mg CE g-1 plant material) contents. The antioxidant activity of CSLE was evaluated using DPPH, H2O2 and reducing power assay. CSLE showed potent antioxidant activity with IC50 value of 0.5±0.26 mg mL-1 and 1.00±0.31 mg mL-1 in DPPH assay and H2O2 study, respectively. The electron donating capability (reducing power) of CSLE was found to be higher than standard antioxidant (vitamin E). The toxicity screening of CSLE was conducted using brine shrimp assay, acute toxicity screening and histopathologic study. An acute toxicity study was carried out by using OECD guideline 423. The LC50 and LD50 values of CSLE were found to be 2.59±0.3 mg mL-1 and > 5000 mg kg-1, respectively. The organ body weight of the CSLE treated mice shows no considerable difference with control group mice. This shows that CSLE does not affect the weight of the mice. No mortality or significant signs of acute toxicity was observed during the 14 days observation period. On the day 14, all the mice were dissected and organ as heart, liver, kidney, spleen and lung were withdrawn for the calculation of organ body index. The organ body index obtained showed that no significant difference with control. Withdrawn organ was subjected to histopathological study. Histopathological analysis of organs did not show any pathological changes. The results obtained in present study indicate that CSLE is very good antioxidant source with high margin of safety.]]>Baruah, A., S. Nath and A. Hazarika,2001Cinnomomum iners reinw.]]>45261263Baumann, J., G. Wurn and F.V. Bruchlausen,1979307R1R77Choi, S.S., K.J. Han, J.K. Lee, H.K. Lee and E.J. Han,200373471485Iida, N., R. Ishii, J. Hakamata, S. Myamoto and H. Oozeki,19971997Krishnaraju, A.V., V.N. Rao-Tayi, D. Sundararaju, M. Vanisree, H.S. Tsay and G.V. Subbaraju,2005Artemia salina) lethality assay.]]>3125134Massele, A.Y. and C.M. Nshimo,199572661663Meyer, B.N., N.R. Ferrigni, J.E. Putnam, L.B. Jacobsen, D.E. Nichols and J.L. McLaughlin,1982453134Miller, M.J., H. Sadowska-Krowicka, S. Chotinaruemol, J.L. Kakkis and D.A. Clark,19932641116Mustaffa, F., J. Indurkar, S. Ismail, M.N. Mordi, S. Ramanathan and S.M. Mansor,2010Cinnomomum iners leaves methanolic extract.]]>27681OECD.,20012001OECD/OCDE,20012001Oyaizu, M.,198644307315Ozsoy, N., A. Can, R. Yanardag and N. Akev,2008Smilax excelsa L. leaf extracts.]]>110571583Pengelly, A.,2004Phutdhawong, W., R. Kawaree, S. Sanjaiya, W. Sengpracha and D. Buddhasukh,2007Cinnamomum iners Reinw. ex Bl.: comparison with conventional hydrodistillation.]]>12868877Ridtitid, W., C. Sae-Wong, W. Reanmongkol and M. Wongnawa,2008Kaempferia galanga Linn. in experimental animals.]]>118225230Sakanaka, S., Y. Tachibana and Y. Okada,200589569575Schimmer, O., F. Haefele and A. Kruger,1988Salmonella typhimurium TA98 and TA 100.]]>206201208Schimmer, O., A. Kruger, H. Paulini and F. Haefele,199449448451Shahidi, F., P.K. Janitha and P.D. Wanasundara,19923267103Subhasree, B., R. Baskar, R.L. Keerthana, R.L. Susan and P. Rajasekaran,200911512131220Tsung, A., A. Rosemary, I.K. Hoffman, N.D. Critchlow and D. Nakao et al.,200517576617668Verma, S. and S.P. Singh,20081347350Omar, W.A., Z.U. Ngah, M.Z. Zaridah and A.N. Rain,2007In vitro and in vivo antiplasmodial properties of some Malaysian plants used in traditional medicine.]]>1697101Brand-Williams, W., M.E. Cuvelier and C. Berset,1995282530Wolfe, K., X. Wu and R.H. Liu,200351609614Zuraini, Z., A. Rais, L.Y. Latha, S. Sasidharan and R. Xavier,2008Coleus blumei, Orthosiphon stamineus, Ocimum basilicum and Mentha arvensis from Lamiaceae family.]]>29395Zou, Y., Y. Lu and D. Wei,2004Hypericum perforatum L. in vitro.]]>5250325039WHO,20052005Ruch, R.J., S.J. Cheng and J.E. Klaunig,19891010031008