International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2010.43.47Catharanthus roseus (Apocynaceae) Crude Extracts and Pure Compounds Against Human Colorectal Carcinoma Cell Line]]>SiddiquiM. J.IsmailZ. AishaA. F.A.Abdul MajidA. M.S.1201061Catharanthus roseus (Apocynaceae), in Malaysia known as Kemuning cina, well known for being rich in alkaloids was investigated for its cytotoxic activity by using MTT assay against Human Colorectal Carcinoma Cell Line (HCT 116). The preliminary cytotoxicity study demonstrated dose independent cytotoxic activity of the methanol extract of C. roseus when screened against HCT-116 colorectal carcinoma cell line. n-hexane, chloroform and methanol fractions also showed dose independent cytotoxic activity with chloroform fraction showing the highest activity. Water fraction showed a minor cytotoxic activity, vindoline also showed some cytotoxic activity at 200 μg mL-1. Catharanthine showed the most promising activity while dose dependent cytotoxic activity of its IC50 value was found to be at 60 μg mL-1. Simple and facile method has been developed for the isolation of compounds catharanthine and vindoline from this plant.]]>Chattopadhyay, R.R., R.N. Banerjee, S.K. Sarkar, S. Ganguly and T.K. Basu,1992Vinca rosea Linn in experimental animals.]]>36291292Chattopadhyay, R.R., S.K. Sarkar, S. Ganguly, R.N. Banerjee and T.K. Basu,1991Vinca rosea Linn.]]>35145151El-Sayed, A. and G.A. Cordell,1981Catharanthus roseus.]]>44289293El-Sayed, A., G.A. Handy and G.A. Cordell,1983Catharanthus roseus.]]>46517527Ghosh, R.K. and I. Gupta,1980Vinca rosea and ficus racemososus on huperglycemia in rats.]]>19145148Gorman, M., N. Neuss and N.J. Cone,1965879399Gorman, M., N. Neuss and K. Biemann,19621, structure of Vindoline.]]>8410581059Johnson, I.S., J.G. Armstrong, M. Gorman and J.P. Burnett,19632313901427Mosmann, T.,1983655563Mukhopadhyay, S. and G.A. Cordell,1981Catharanthus trichophyllus and pericyclivine from Catharanthus roseus.]]>44335339Nobili, S., D. Lippi, E. Witort, M. Donnini, L. Bausi, E. Mini and S. Capaccioli,200959365378Padua, De L.S., N. Bunyapraphatsara and R.H.M.J. Lemmens,199912411412Sapi, J. and G. Massiot,19941994pp: 523-646pp: 523-646Singh, S.N., P. Vats, S. Suri, R. Shyam, M.M.L. Kumria, S. Ranganathan and K. Sridharan,2001Catharanthus roseus on enzymic activities in streptozotocin induced diabetic rats.]]>76269277Taylor, W.I. and N.R. Farnsworth,19751st Edn.,Ueda, J.Y., Y. Tezuka, A.H. Banskota, Q. Le Tran and Q.K. Tran et al.,200225753760Verma, A., I. Laakso, T. Seppanen-Laakso, A. Huhtikangas and M. Riekkola,2007Catharanthus roseus combined with a semi-synthetic production process for vinblastine.]]>1213071315Williams, C.A. and R.J. Grayer,200421539573Chattopadhyay, R.R.,199967367372