International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2010.290.295Neolitsea aciculata Extract in Murine B16 F10 Melanoma]]>KimS. S.YoonW. J.HyunC. G.LeeN. H.3201063Melanogenesis is a well known physiological response of human skin and is mainly caused by ultraviolet irradiation and some genetic factors. In our efforts to find new skin lightening agents, Neolitsea aciculata butanol fraction (NAB) was investigated for its ability to inhibit melanogenesis. In this study, NABs effect on inhibition of mushroom tyrosinase was assessed, which indicated its good anti-tyrosinase activity (IC50 211.4 Fg mL-1). Furthermore, NAB was shown to down-regulate melanin content on a dose dependent pattern. To clarify the target of its action in melanogenesis, Western blotting for tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2 and microphthalmia-associated transcription factor (MITF) was performed. As a result, NAB inhibited tyrosinase, TRP-2 and MITF expressions in a dose-dependent manner. However, it did not inhibit TRP-1 expression. In order to evaluate its potential in cosmetic applications, NABs cell toxicity was also determined by MTT assays using human dermal fibroblast cells. The NAB exhibited low cytotoxicity at 100 μg mL-1 in human cell line. Therefore, this study suggests that NAB is a potential anti-melanogenic agent and might be effective in treatment of hyperpigmentation disorders.]]>Abbott, C., I.J. Jackson, B. Carritt and S. Povey,199111471473Busca, R. and R. Ballotti,2000136069Espin, J.C., M. Morales, R. Varon, J. Tudela and G.C. Francisco,2006611771182Friedmann, P.S. and B.A. Gilchrest,19871338894Friedmann, P.S., F.E. Wren and J.N. Mattews,1990142334341Fuse, N., K. Yasumoto, H. Suzuki, K. Takahashi and S. Shibahara,1996219702707Huang, Y.H., T.H. Lee, K.J. Chan, F.L. Hsu, Y.C. Wu and M.H. Lee,200849115123Karioti, A., A. Protopappa, N. Megoulas and H. Skaltsa,2007Marrubium velutinum and Marrubium cylleneum.]]>1527082714Ko, R.K., M.C. Kang, B.S. Kim, J.H. Han, K.O. Kim and N.H. Lee,2009Aster subulatus Michx.]]>3011671169Ko, R.K., S. Lee, C.G. Hyun and N.H. Lee,2009Distylium racemosum Sieb. et Zucc.]]>3013761378Kobayashi, T., K. Urabe, A. Winder, C. Jimenez-Cervantes and G. Imokawa et al.,19941358185825Laskin, J.D. and L.A. Piccinin,19862611662616635Kazuhisa, M. and F. Minoru,1991In vitro effectiveness of several whitening cosmetic components in human melanocytes.]]>42361368Mosmann, T.,1983655563Okombi, S., D. Rival, S. Bonnet, A. Mariotte, E. Perrier and A. Boumendjel,20061622522255Page, B., M. Page and C. Noel,1993in vitro.]]>3473476Park, S.H., D.S. Kim, W.G. Kim, I.J. Ryoo and D.H. Lee et al.,20046128782885Slominski, A., D.J. Tobin, S. Shibahara and J. Wortsman,20048411551228Sultan, N. and N.H. Lee,2009Sasa quelpaertensis Nakai with tyrosinase inhibition activities.]]>3017291732Tief, K., M. Hahne, A. Schmidt and F. Beermann,19962411216Tsukamoto, K., I.J. Jackson, K. Urabe, P.M. Montague and V.J. Hearing,199211519526Udono, T., K.I. Yasumoto, K. Takeda, S. Amae and K.I. Watanabe et al.,20001491205219Wu, H. and H.Y. Park,2003311948953Yokoyama, K., H. Suzuki, K. Yasumoto, Y. Tomita and S. Shibahara,19941217317321