International Journal of Pharmacology1811-77751812-5700Asian Network for Scientific Information10.3923/ijp.2009.162.167Orthosiphon stamineus Benth. Methanolic Leaves Extract]]>SahibH.B. AishaA.F. YamM.F. AsmawiM.Z. IsmailZ. SalhimiS.M. OthmanN.H. Abdul MajidA.M.S. 2200952Angiogenesis is a process by which new blood vessels are formed from the pre-existing blood vessel. Orthosiphon stamineus Benth. OS has been used as a medicinal herb for many centuries. Due to the presence of high level of anti-oxidants and phenolic content compounds in OS and the effect of anti-oxidants and phenolic compounds being anti-angiogenic, the perturbation of new blood vessels ability of OS was tested. Dry powdered leaves of the OS plant were extracted with Petroleum Ether (PE), chloroform (CE), methanol (ME) and water (WE) by using sequential cold maceration method. The ME of OS has the highest anti-angiogenic activity (93.28±1.24%) in the rat aortic assay followed by CE (85.55±1.64%), PE (51.54±4.12%) and WE (50.22±1.23%) in descending order of reactivity. The methanol extract was also found to have potent anti-oxidant activity in the1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. The IC50 value was measured to be 0.286 mg mL-1. The total phenolic content of 1 mg mL-1 of methanol extract was equal to 38.27%.]]>Akowuah, G.A., I. Zahari, I. Norhayati, A. Sadikun and M.S. Khamsha,200482556559Bagchi, D., C.K. Sen, M. Bagchi and M. Atalalay,20046995102Beaux, D., J. Fleureantin and F. Morticr,1999Orthosiphon stamineus Benth, Hieracium pilosella, Sambucus nigra L and Actostaphylos Ura-ursi (L.) Spreng in rats.]]>13222225Brown, K.J., S.F. Maynes, A. Bezos, D.J. Maguire, M.D. Ford and C.R. Parish,1996in vitro assay for human angiogenesis.]]>75539555Carmeliet, P., V. Ferreira and G. Bereier,1996380435439Eengelmann, C., E. Blot, Y. Panis, S. Bauer, V. Trochon, H. Nagy, H.J. Lu and C. Soria,2002In vitro contrasted by lack of efficacy in vivo.]]>9489495Folkman, J.,199528511681182Fotsis, T., M.S. Pepper, E. Aktas, S. Breit and S. Rasku et al.,1997in vitro angiogenesis.]]>5729162921Goodwin, A.M.,2007In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents.]]>74172183Tsuda, H., Y. Ohshima, H. Nomoto, K. Fujita and E. Matsuda et al.,200419245263Jaganth, I. and L. Ng,2000pp: 76-77pp: 76-77Kerbel, R.S.,200021505515Kim, H.,2003in vitro by inhibiting the matrix-degrading proteases.]]>89529538Matsubara, T.,1999OrthSiphon arisatus and Indoniesian traditional plants.]]>2210831088Mukherjee, P., J.R. Zhou, A.V. Sotnikov and S.K. Clinton,19991999Nicosia, R.F., Y.J. Lin, D. Hazelton and X. Qian,199715113791386Oktay, M., I. Gulcin and O.I. Kufrevioglu,2003in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts.]]>36263271Peter, C. and K.J. Rakesh,2000407249256Robert, A., L. Rachel, S. Brenda, K. Louis and A. Nasim,2003493240Shklar, G.,1998342429Slinkard, K. and V.L. Singleton,1977284955Tan, Y., R. Yu and J.M. Pezzuto,2003928662875Tezuka, Y., P. Stampoulis, A.H. Banskota, S. Awale, K.Q. Tran, I. Saiki and S. Kadota,2000Orthosiphon stamineus.]]>4817111719Yam, M.F., L.F. Ang and R. Basir,20062006