International Journal of Dairy Science1811-97431811-9751Asian Network for Scientific Information10.3923/ijds.2017.282.288SusmiatiTrini PutroPrabowo Purwono Sarmin AirinClaude Mona Hartiningsih SatriaPamungkas Bagus 42017124Background and Objective: Polymorphisms of kappa-casein protein genes are closely related to the quality of milk production and composition. The purpose of the study was to investigate polymorphisms in genes encoding kappa-casein (κ-casein) milk protein produced by the dairy cattle of KP4/PIAT UGM Yogyakarta and BBPTU-HPT Baturraden. Materials and Methods: Twenty-three cattle were used to investigate kappa-casein polymorphisms in dairy cows from two different animal farms. Nine cattle were distributed from BBPTU-HPT Baturraden, Central Java and 14 cattle were allocated from KP4/PIAT UGM Yogyakarta. The research procedures included blood sampling, DNA extractions and DNA amplification using Polymerase Chain Reaction (PCR). Kappa-casein gene PCR products were analyzed by using the PCR-RFLP technique with restriction enzymes Hinf I and Hind III. Results: The results of restriction digestion using the Hinf I showed four restriction fragments in the blood sample from BPPTU-HPT Baturraden and three restriction fragments from KP4/PIAT UGM Yogyakarta. The digestion of PCR product using Hind III produced one restriction fragment in the blood samples from KP4/PIAT UGM Yogyakarta and 4 fragments in the samples from BPPTU-HPT Baturraden. Conclusion: The dairy cattle of BPPTU Baturraden offer a more favorable genetic selection of animals for milk production and industry due to their genetic variants in terms of genotypes and alleles.]]>Sutarno, S. and A.D. Setyawan,201516327354 Kucerova, J., A. Matejicek, O.M. Jandurova, P. Sorensen, E. Nemcova, M. Stipkova and J. Frelich,2006CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh.]]>51241247Anggraeni, A.,201322111Rachagani, S. and I.D. Gupta,200831893897Riaz, M.N., N.A. Malik, F. Nasreen and J.A. Qureshi,200828103106Sulimova, G.E., M. Ahani-Azari, J. Rostamzadeh, M.R. Mohammad Abadi and O.E. Lazebny,2007437379Bhat, M.Y., T.A. Dar and L.R. Singh,20162016pp: 3-18pp: 3-18Choobini, Z.M., M. Shadkhast, H. Moshtaghi, S.H. Dehkordi and H.R. Shahbazkia,2014125660Marrero, N.M., L.A. de Mascena, J.L. de Macedo, A.M. Valdes, M.A.G. Filho and O.U. Reinosa,2016387178Byrne, G.,200723247Stafuzza, N.B., K.J. Kochan, P.K. Riggs and M.E.J. Amaral,201332675679Azevedo, A.L.S., C.S. Nascimento, R.S. Steinberg, M.R.S. Carvalho, M.G.C.D. Peixoto, R.L. Teodoro and M.A. Machado,20087623630Botaro, B.G., Y.V.R. de Lima, C.S. Cortinhas, L.F.P. e Silva, F.P. Renno and M.V. dos Santos,20093824472454Trakovicka, A., N. Moravcikova and A. Navratilova,2012156164Dedovic, R., V. Bogdanovic, P. Perisic, D. Stanojevic, J. Popovic and M. Brka,2015472332Cortes-Lopez, N.G., S. del Moral, J.A. Rueda, C. Luna-Palomera, C.A. Meza-Herrera and J. Abad-Zavaleta,2012154755Anggraini, A., C. Sumantri, A. Farajallah and E. Andreas,2010336167Memon, I.A., M.A. Raza, W.A. Vistro, M.F. Leghari and A.W. Nizamani et al.,2016Bos indicus Sindhi genetic group using HINFI restriction enzyme.]]>23439Sitkowska, B., N. Wojciech and W. Ewa,20089641644Alipanah, M., L. Kalashnikova and G. Rodionov,20075158161