International Journal of Dairy Science1811-97431811-9751Asian Network for Scientific Information10.3923/ijds.2011.314.322Enterococcus faecium VL47 Strain in the Presence of Prebiotics]]>VamanuEmanuel 6201166The biochemical characterization of the bacteriocine synthesized by Enterococcus faecium VL47 was realized, resulting a high thermal stability, resistance to acid pH, organic solvents and to the used enzymes. The production of antimicrobial peptides (bacteriocines) was used against three bacterial strains, Bacillus cereus CMGB 215, Listeria innocua CMGB 218, Escherichia coli CBAB2. The synthesis of bacteriocines was tested by using the MRS medium, thus, resulting an inhibiting effect against all three sensitive strains, even if the carbon source was replaced by lactose, sucrose, sorbitol, sorbose, arabinose, maltose, galactose and trehalose. The inhibiting effect of the precipitated bacteriocine was obtained when using a concentration of ammonium sulphate, with an average diameter of 1 cm against sensitive strains. To outline the effect of prebiotics on bacteriocine synthesis, MRS was supplemented by 1% inulin from Chichori, inulin from Dahlia, lactulose, raffinose, stachyose, xylose. It resulted that the maximum inhibiting effect was obtained when using lactulose, in a concentration of 1%.]]>Atta, H.M., B.M. Refaat and A.A. El-Waseif,2009Lactobacillus plantarum, NRRL B-227.]]>4115125Cabo, M.L., M.A. Murado, M.P. Gonzalez and L. Pastoriza,199987907914Alvarez-Cisneros, Y.M., F.J. Fernandez, C. Wacher-Rodarte, M.B. Aguilar, T. del Rosario Sainz Espunes and E. Ponce-Alquicira,2010Enterococcus faecium MXVK29, isolated from Mexican traditional sausage.]]>9024752481Jagadeeswari, S., P. Vidya, D.J.M. Kumar and M.D. Balakumaran,2010Lactobacillus sp. from traditional fermented foods.]]>9575581Karthikeyan, V. and S.W. Santhosh,2009L. acidophilus strain as probiotic.]]>8335340Khalil, R., Y. El-Bahloul, F. Djadouni and S. Omar,2009Bacillus megaterium strain 19.]]>8242250Martinez, R.C.R. and E.C.P. De Martinis,2005Lactobacillus sakei 1 against Listeria monocytogenes 1/2a growth and haemolytic activity.]]>368387Norwood, D.E. and A. Gilmour,1999Listeria monocytogenes strains to stainless steel coupons.]]>86576-58210212403Sharma, S., A.P. Garg and G. Singh,2010Lactococcus lactis CCSULAC1 on modified MRS medium.]]>519Vamanu, E., A. Vamanu and D. Pelinescu,2010Lactobacillus paracasei YR strain.]]>61502505Vijayendra, S.V.N., K. Rajashree and P.M. Halami,2010Enterococcus faecium isolated from idli batter.]]>50243246