International Journal of Dairy Science1811-97431811-9751Asian Network for Scientific Information10.3923/ijds.2008.55.62Abd El-RazikK.A. IsmailE.M. YoussefH.M. HashadM.A. 2200832In this study, a total number of 92 milk samples collected from obligatory slaughtered 32 cattle, 30 buffaloes and 30 sheep suspected for brucellosis in different governorates of Egypt were tested using the nested Polymerase Chain Reaction (nPCR). Thirty eight out of the 92 milk samples (41.3%) gave the expected product. The sensitivity of nested PCR using vaccinal strains S19 and Riv I vaccine, ranged between as high as 10-102 cfu mL-1. The nesting of PCR resulted in rise of PCR sensitivity, by about 10 folds. Bacteriological studies yielded 16 (17.3%) Brucella isolates recovered from milk (6 from cattle, 4 from buffaloes and 6 from sheep), they were typed as Brucella melitensis biovar 3. It could be concluded that using nested PCR as confirmatory test for detection of susceptible dairy animals, is of great value to avoid condemnation of false positive reactors, which reflects economically on animal resources.]]>Abd El-Razik, K.A., H.M. Desoukey and W.M. Ahmed,2006Brucella melitensis.]]>10219234Abd El-Razik, K.A., H.M. Desoukey and W.M. Ahmed,200710342348El-Razik, K.A., Y.A. Ghazi and E.M. Salama,2007brucella reactor does following milk examination using different techniques.]]>10240244Alton, G.G., L.M. Jones, R.D. Angus and J.M. Verger,19881st Edn.,Pages: 174Pages: 174Alton, G.G.,1990Brucella melitensis 1887 to 1987.]]>1990Amin, A.S., H.S. Husseinen, G.S. Radwan, M.N.H. Shalaby and N. El-Danaf,1995Brucella antigen in field samples.]]>55761787Baily, G.G., J.B. Krahn, B.S. Drasar and N.G. Stoker,1992Brucella melitensis and Brucella abortus by DNA amplification.]]>95271275Blasco, J.M.,1992Brucella Melitensis Infections in Small Ruminant.]]>1992Cetinkaya, B., H. Ongor, A. Muz, H.B. Ertas, H. Kalender and H.M. Erdogan,1990Brucella species DNA in the stomach content of aborted sheep fetuses by PCR.]]>47227229Cetinkaya, B., K. Egan, D.A. Harbour and K.L. Morgan,199661373379Collins, D.M., D.M. Stephens and G.W. Delisle,1993Mycobacterium paratuberculosis in bovine faeces.]]>36289289Farrel, I.D.,1974Brucella abortus from contaminated sources.]]>16280280Fekete, A., J.A. Bantle and S.M. Halling,1990Brucella by polymerase chain reaction in bovine fetal and maternal tissues.]]>47983Fekete, A., J.A. Bantle, S.M. Halling and R.W. Stich,1992Brucella strains by use of polymerase chain reaction with arbitrary primers.]]>17477787783Fekete, A., M.R. Sanborn, S.M. Halling and J.A. Bantle,19921992pp: 467-468pp: 467-468Ghazi, Y.A., K.A. Abd EL-Razik and M.B. Kadry,2006Brucella diagnostic techniques in the Egyptian buffaloes.]]>2006pp: 2334Herman, L. and H. de Ridder,1992Brucella spp. by using the polymerase chain reaction.]]>5820992101Husseinen, H.S.,1993Brucella species at the OMP2 Locus.]]>1993Kulakov, Y.K., V.N. Gorelov, V.L. Motin, G.V. Brukhanskii and A.G. Skavronskaia,1992Brucella.]]>7-82327Leal-Klevezas, D.S., I.O. Martinez-Vazquez, A. Lopez-Merino and J.P. Martinez-Soriano,1995Brucella sp. from blood and milk of infected animals. ]]>3330873090Matar, G.M., I.A. Khneisser and A.M. Abdelnoor,1996Brucella antigen DNA.]]>34477478Mercier, E., E. Jumas-Bilak, A. Allardet-Servent, D. O'Callaghan and M. Ramuz,1996Brucella strains detected by studying distribution of two short repetitive DNA elements.]]>3412991302Moriyon, I. and D.T. Berman,1982Brucella cell envelope.]]>152822828Persing, D.H.,19912912811285Rafi, A., H.D. Donoghue and J.L. Stanford,1995634247Rijpens, N.P., G. Jannes, M.V. Asbroeck, R. Rossau and L.M.F. Herman,1996Brucella sp. in raw milk by PCR and reverse hybridizaiton with 16S-32S rRNA spacer probes.]]>1996Romero, C. and I. Lopez-Goni,1999Brucella sp. by PCR.]]>1999Romero, C., C. Gamazo, M. Pardo and I. Lopez-Goni,1995Brucella DNA by PCR.]]>33615617Romero, C., M. Pardo, M.J. Grillo, R. Diaz, J.M. Blasco and I. Lopez-Goni,19953331983200Vanzini, V.R., N. Aguirre, C.I. Lugaresi, S.T. de Echaide and V.G. de Canavesio et al.,199836211217Verger, M., F. Grimont, P.A.D. Grimont and M. Grayon,1985Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridizat.]]>35292295