International Journal of Dairy Science1811-97431811-9751Asian Network for Scientific Information10.3923/ijds.2007.116.125Bifidobacterium Supernatants Towards Growth of Bacillus cereus and Escherichia coli]]>CheikhyoussefA. PogoriN. ZhangH. 2200722The antimicrobial effects of the supernatants obtained from four strains of bifidobacteria were tested against two types of indicator strains, Bacillus cereus and three strains of Escherichia coli. E. coli AS 1.543 was the most sensitive strain among the E. coli strains whereby B. bifidum showed the highest antimicrobial activity against indicator strains followed by B. infantis then B. longum and finally B. adolescentis. There was a significant reduction in the growth of the indicator strains, whereby all E. coli strains were inhibited more than 75% of their log CFU in monoculture, of which B. infantis reduced 95% of E. coli AS 1.543. On the other hand only more than 60% of the log CFU of B. cereus was reduced by all bifidobacterial supernatants in particular B. longum (75%). It was observed that substances or factors other than organic acids may contribute to the antimicrobial activity of the supernatants (CFS) from the bifidobacteria studied. Neutralization of the CFS from B. adolescentis and B. bifidum significantly reduced the antimicrobial activity while that from B. infantis and B. longum continued to inhibit growth of the indicator strain as observed by a clear defined zone uncharacteristic of acid production by the agar diffusion method. The inactivation of the antimicrobial activity of CFS from B. infantis and B. longum by proteolytic enzymes confirms the proteinaceous nature of the antimicrobial compound present. In addition their heat and pH stability further proves the presence of a proteinaceous antimicrobial compound which could be designated as bacteriocins or bacteriocin-like compounds.]]>Anned, S.K., R.A. Sreenivasan and L.K. Rao,1985Bifidobacterium bifidum-I I.]]>22123Ballongue, J.,19981998pp: 519-587pp: 519-587Bruno, F.A. and N.P. Shah,2002Bifidobacterium sp.]]>57617621Collado, M.C., M. Hernandez and Y. Sanz,2005Bifidobacterium strains.]]>6810341040De Man, J.C., M. Rogosa and M.E. Sharpe,1960Lactobacilli.]]>23130135De Vuyst, L., L. Avonts and L. Makras,20042004pp: 416-482pp: 416-482Fuller, R.,198966365378Geis, A., J. Singh and M. Tueber,198345205211Gibson, G.R. and X. Wang,199477412420Glass, K.A., J.M. Loeffelholz, J.P. Ford and M.P. Doyle,1992Escherichia coli O157:H7 as affected by pH or sodium chloride and in fermented, dry sausage.]]>5825132516Ibrahim, S.A. and M.M. Salameh,2001Bifidobacterium species of human isolates.]]>95263Ibrahim, S. and A. Bezkorovainy,1993Escherichia coli by bifidobacteria.]]>56713715Ivanova, I., P. Kabadjova, A. Pantev, S. Danova and X. Dousset,2000Lactococcus lactis subsp. lactis B14 isolated from Boza-Bulgarian traditional cereal beverage.]]>414753Kang, K.H., H.J. Shin, Y.H. Park and T.S. Lee,1989B. longum.]]>11204216Kramer, J.M. and R.J. Gilbert,1989Bacillus Cereus and other Bacillus Species.]]>1989pp: 21-77pp: 21-77Larsen, H.D. and K. Jorgensen,1997Bacillus cereus in danish pasteurized milk.]]>347179186Maher, M.M., K.N. Jordan, M.E. Upton and A. Coffey,2001Escherichia coli O157:H7 during the manufacture and ripening of a smear-ripened cheese produced from raw milk.]]>90201207Makras, L. and L. De Vuyst,2006in vitro inhibition of Gram-negative pathogenic bacteria by bifidobacteria is caused by the production of organic acids.]]>1610491057Meghrous, J., P. Euloge, A.M. Junelles, J. Ballongue and H. Petitdemange,1990Bifidobacterium strains for bacteriocin production.]]>12575580Rioradan, K.O. and G.F. Fitzgerald,199885103114Tagg, J.R., A.S. Dajani and L.W. Wannamaker,197640722756Trejo, F.M., J. Minnaarda, P.F. Pereza and G.L. De antoni,2006Clostridium difficile growth and adhesion to enterocytes by Bifidobacterium supernatants.]]>12186193Yildirim, Z. and M.G. Johnson,1998Bifidobacterium bifidum NCFB 1454.]]>614751Zhao, T. and M.P. Doyle,1994Escherichia coli O157: H7 in commercial mayonnaise.]]>57780783