International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2020.40.47AlqatatiAbeer S. Hamad ElgazwyAbdel-Sattar AliwainiSaeb 22020162Background and Objective: Palladacycles have been reported to exert significant anticancer activities against different cancer cells. The current study conducted to evaluate the anti-tumor activity of a new palladacycle complex (ASH10) in estrogen receptor-positive (MCF7) and triple-negative (HCC1937) breast cancer cells. Materials and Methods: The effect of ASH10 on cell proliferation was tested by MTT assay. Scratch assay was used to test the anti-migration ability of ASH10. Apoptosis induced by ASH10 was measured by different methods including nuclear staining and detection of apoptosis markers by western blotting. Autophagy induced by ASH10 also measured by LC3II puncta staining and western blotting using the LC3II antibody. Results: The MTT results showed that the ASH10 compound has a strong anti-growth effect. Data showed that ASH10 induces its cytotoxic effect by inducing DNA damage followed by cell cycle arrest, intrinsic apoptosis and autophagy. Compared to untreated cells, ASH10 treated cells showed high levels of DNA damage markers p-H2AX, p-ATM and p53. Importantly, the cell cycle arrest marker p21 was similarly induced by ASH10 treatment. Furthermore, ASH10 induced significant levels of apoptosis as evidenced by nuclear fragmentation and an increase in the levels of PARP cleavage. Interestingly, ASH10 also activated the formation of autophagosomes and increased the autophagy marker LC3II. Inhibition of autophagy led to a decrease in ASH10 cytotoxicity suggesting that ASH10 induced autophagy was a cytotoxic mechanism. Conclusion: These results demonstrated that ASH10 is a potential effective compound in the treatment of both ER-negative and ER-positive breast cancer cells.]]>Siegel, R.L., K.D. Miller and A. Jemal,202070730Aliwaini, S., J. Bleloch, S. Kimani and S. Prince,20162016pp: 231-247pp: 231-247Aliwaini, S., A.J. Swarts, A. Blanckenberg, S. Mapolie and S. Prince,2013in vitro and in vivo through apoptosis and autophagy.]]>8616501663Aliwaini, S., J. Peres, W.L. Kroger, A. Blanckenberg and J. de la Mare et al.,2015357206218Blanckenberg, A., S. Aliwaini, S.W. Kimani, A. van Niekerk, A. Neumann-Mufweba, S. Prince and S.F. Mapolie,20178516878Kruszewski, M., E. Bouzyk, T. Oldak, K. Samochocka and L. Fuks et al.,200323111Gigli, R., G.J.S. Pereira, F. Antunes, A. Bechara and D.M. Garcia et al.,2016107245254Elgazwy, A.S.S.H.,200813912851297Al-Qatati, A. and S. Aliwaini,20171479937999Aliwaini, S., A.M.Awadallah, R.Y. Morjan, M. Ghunaim and H. Alqaddi et al.,201918830837Fulda, S. and K.M. Debatin,20062547984811Pajaniradje, S., K. Mohankumar, R. Pamidimukkala, S. Subramanian and R. Rajagopalan,2014Sesbania grandiflora leaves in human cancer cells.]]>2014Ulukaya, E., F. Ari, K. Dimas, E.I. Ikitimur, E. Guney and V.T. Yilmaz,2011in vitro and in vivo.]]>4649574963Tanaka, M., H. Kataoka, S. Yano, H. Ohi and K. Kawamoto et al.,20132013Pandy, J.G.P., J.C. Balolong-Garcia, M.V.B. Cruz-Ordinario and F.V.F. Que,20192019Tassone, P., P. Tagliaferri, A, Perricelli, S, Blotta and B. Quaresima,20038812851291Corkery, B., J. Crown, M. Clynes and N.O. Donovan,200920862867Viola, G., R. Bortolozzi, E. Hamel, S. Moro and P. Brun et al.,2012831626Pan, X., X. Zhang, H. Sun, J. Zhang, M. Yan and H. Zhang,20132013Abedin, M.J., D. Wang, M.A. Mcdonnell, U. Lehmann and A. Kelekar,200714500510Liu, L., M. Yang, R. Kang, Z. Wang and Y. Zhao et al.,20117112114Xu, Y., H. Yu, H. Qin, J. Kang and C. Yu et al.,2012314232243Shin, S.W., S.Y. Kim and J.W. Park,20121823451457Xiong, H.Y., X.L. Guo, X.X. Bu, S.S. Zhang and N.N. Ma et al.,20102886874Shen, Z.X., G.Q. Chen, J.H. Ni, X.S. Li and S.M. Xiong et al.,19978933543360Soignet, S.L., P. Maslak, Z.G. Wang, S. Jhanwar and E. Calleja et al.,199833913411348Kanzawa, T., Y. Kondo, H. Ito, S. Kondo and I. Germano,20036321032108Rousselot, P., S. Labaume, J.P. Marolleau, J. Larghero, M.H. Noguera, J.C. Brouet and J.P. Fermand,19995910411048Jung, S.Y., S.. Lee, H.B. Kang, H.A. Park and S.K. Chang et al.,20102066336636Andreopoulou, E. and J.A. Sparano,201354250Xi, G., X. Hu, B. Wu, H. Jiang, C.Y.F. Young, Y. Pang and H. Yuan,2011307141148Veldhoen, R.A., S.L. Banman, D.R. Hemmerling, R. Odsen and T. Simmen et al.,201332736746