International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2020.18.27Manihot esculenta Cranz) on Raji Cells]]>SutiningsihDwi Arie WuryantoMohamad Setyawan SusantoHenry HariyadiSujud Mustofa 12020161Background and Objective: Linamarin is an active compound isolated from the leaves of cassava (Manihot esculenta Cranz) that has cytotoxic effects on HT-29, MCF-7 and HL-60 cells. This study was aimed to determine the cytotoxic and antiproliferation activity and induction of p53 protein in Raji cells after administration of various concentrations of linamarin from cassava leaves (Manihot esculenta Cranz). Materials and Methods: Linamarin was isolated from cassava leaves (Manihot esculenta Cranz) using a multilevel purification method. Linamarin cytotoxicity was tested on Raji cells using the MTT method, while antiproliferation activity was tested using a doubling time test. The p53 protein expression was observed by immunocytochemical tests. The cytotoxic activity of Raji cells was expressed by the value of Inhibitory concentration 50 μg mL1. The doubling time was calculated by comparing the slope values of the log graphs of the number of cells at various times. Raji cells that were positive for p53 protein showed brown painted nuclei or cytoplasm. Results: Linamarin from cassava leaves can inhibit cytotoxic activity and proliferation on Raji cells. The higher the linamarin concentration, the longer the doubling time of Raji cells. The expression of p53 protein on Raji cells after linamarin administration was higher than the control. The p53 protein expression was found in the nuclei (91.05%) and cytoplasm (8.95%). Conclusion: Based on those findings, linamarin from cassava leaves has the potential to be developed as an anticancer agent.]]>Akinpelu, A.O., L.E.F. Amamgbo, A.O. Olojede and A.S. Oyekale,201111118125Adenle, A.A., O.C. Aworh, R. Akromah and G. Parayil,20122012Ernesto, M., A.P. Cardoso, D. Nicala, E. Mirione and F. Massaza et al.,200282357362Sayre, R., J.R. Beeching, E.B. Cahoon, C. Egesi and C. Fauquet et al.,201162251272Al Fourjani, W.A.,2005In vitro anticancer properties of linamarin controlled release from biodegradable poly-lactic co-glycolic acid nanoparticle.]]>2005Idibie, C.A., H. Davids and S.E. Iyuke,200730261269Yusuf, U.F., F.R. Ahmadun, R. Rosli, S.E. Iyuke, N. Billa, N. Abdullah and N. Umar-Tsafe,2006in vitro inhibition of human malignant cell growth of crude water extract of cassava (Manihot esculenta Crantz) and commercial linamarin.]]>28145155Haque, M.R. and J.H. Bradbury,199967305309Girald, W., A. Collin and M. Izquierdo,201131399107Dorgan, J.F., A. Sowell, C.A. Swanson, N. Potischman, R. Miller, N. Schussler and H.E. Stephenson Jr.,199898997Cortes, M.L., V. Garcia-Escudero, M. Hughes and M. Izquierdo,20024407414Rivadeneyra-Dominguez, E., A. Vazquez-Luna, J.F. Rodriguez-Landa and R. Diaz-Sobac,2013Manihot esculenta Crantz) consumption.]]>59230235Van Duijnhoven, F.J.B., H.B. Bueno-De-Mesquita, P. Ferrari, M. Jenab and H.C. Boshuizen et al.,20098914411452Gloria, N.F., N. Soares, C. Brand, F.L. Oliveira, R. Borojevic and A.J. Teodoro,20143413771386Levrero, M., V. de Laurenzi, A. Costanzo, J. Gong, J.Y. Wang and G. Melino,200011316611670Enger, S.M., M.P. Longnecker, M.J. Chen, J.M. Harper, E.R. Lee, H.D. Frankl and R.W. Haile,19965147153Kontek, R., B. Kontek and K. Grzegorczyk,2013in vitro.]]>910061012Mosmann, T.,1983655563Wikanta, T., M. Rasyidin, L. Rahayu and A. Pratitis,2012Ulva fasciata Delile ethyl acetate extract against CaSki and MCF-7 cell lines].]]>78796, (In Indonesian)Itharat, A and B. Ooraikul,200737287317He, Y., Q. Zhu, M. Chen, Q. Huang and W. Wang et al.,201650) of cisplatin: A pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer.]]>77080370821Jorgensen, K., A.V. Morant, M. Morant, N.B. Jensen and C.E. Olsen et al.,2011155282292Lane, D.P., C.F. Cheok and S. Lain,20102010Afsar, T., J.H. Trembley, C.E. Salomon, S. Razak, M.R. Khan and K. Ahmed,2016Acacia hydaspica: Involvement of multiple signal transduction pathways.]]>2016Lehmann, B.D., J.A. Bauer, X. Chen, M.E. Sanders, A.B. Chakravarthy, Y. Shyr and J.A. Pietenpol,201112127502767Khan, N., F. Afaq, M. Saleem, N. Ahmad and H. Mukhtar,20066625002505Ghate, N.B., B. Hazra, R. Sarkar and N. Mandal,2014Acacia catechu induces apoptosis in human breast carcinoma by altering bax/bcl-2 ratio.]]>102730Catz, S.D. and J.L. Johnson,20012073427351Bolhassani, A., A. Khavari, S.Z. Bathaie,201418452030Duarte, T.L. and J. Lunec,200539671686Verma, R.S., A. Mehta and N. Srivastava,2007In vivo chlorpyrifos induced oxidative stress: Attenuation by antioxidant vitamins.]]>88191196Szarka, A., B. Tomasskovics and G. Banhegyi,20121344584483Bindhumol, V., K.C. Chitra and P.P. Mathur,2003188117124Winkler, B.S., S.M. Orselli and T.S. Rex,199417333349Griffiths, H.R. and J. Lunec,200110173182Finlay, C.A., P.W. Hinds and A.J. Levine,19895710831093Oraiopoulou, M.E., E. Tzamali, G. Tzedakis, A. Vakis, J. Papamatheakis and V. Sakkalis,2017In vitro/in silico study on the role of doubling time heterogeneity among primary glioblastoma cell lines.]]>2017Atuegwu, N.C., L.R. Arlinghaus, X. Li, A.B. Chakravarthy, V.G. Abramson, M.E. Sanders and T.E. Yankeelov,20136256264Bertuzzi, A., A. Gandolfi, C. Sinisgalli, G. Starace and P. Ubezio,1997293440Lowe, S.W.,199964548Rivlin, N., R. Brosh, M. Oren and V. Rotter,20112466474Sugerman, P.B. and N.W. Savage,199944147156Petitjean, A., E. Mathe, S. Kato, C. Ishioka, S.V. Tavtigian, P. Hainaut and M. Olivier,2007TP53 mutation patterns and tumor phenotype: Lessons from recent developments in the IARC TP53 database.]]>28622629Hainaut, P. and M. Hollstein,19997781137Schlomm, T., L. Iwers, P. Kirstein, B. Jessen and J. Kollermann et al.,20082113711378MacDonald, F. and C.H.J. Ford,1996pp: 53-60pp: 53-60Nozaki, M., M. Tada, H. Kobayashi, C.L. Zhang and Y. Sawamura et al.,1999p53 and other genes in astrocytoma tumorigenesis and progression.]]>1124137Oren, M. and V. Rotter,20102010Groeger, A.M., V. Esposito, A. de Luca, R. Cassandro and G. Tonini et al.,2004L in resected non-small-cell lung cancers.]]>445463Shaulsky, G., N. Goldfinger, M.S. Tosky, A.J. Levine and V. Rotter,1991620552065Burck, K.B., E.T. Liu and J.W. Larrick,1988pp: 87-99pp: 87-99McManus, E.J. and D.R. Alessi,20042004