International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2019.47.57MohammedMohammed Amin OmarNesreen Moustafa MohammedSoad Amin AminAhmed Mohammed 22019152Background and Objective: MicroRNAs had been implicated in several malignancies. Abnormal circulating microRNA-1246 expression had been detected in HCC patients in an expanding number of studies. However, the information in literature describing the pertinent ramifications of miR-1246 in early-stage HCCs are rare and heterogeneous. This study was designed to assess the diagnostic accuracy of serum miR-1246 level in early-stage HCV-related HCC patients contrasted with chronic hepatitis C (CHC), liver cirrhosis (LC) and healthy control (HC). Materials and Methods: Two hundred HCV outpatients were doled out into 3 groups, HCC group (n = 100), CHC group (n = 30) and LC group (n = 70). Another hundred (100) age- and sex-matched healthy controls (HC) were likewise enlisted. The serum expression level of miR-1246 (by quantitative Real-Time PCR), AFP and prothrombin induced by vitamin K absence-II (PIVKA-II) were tested. Results: In HCC patients, in contrast to AFP, the serum expression levels of PIVKA-II and miR-1246 were statistically significantly increased discriminating HCC patients and early-stage HCCs not only from non-HCC patients (CHC, LC) yet additionally from HC. PIVKA-II and miR-1246, either individually or combined, had excellent diagnostic accuracies and performances as demonstrated by their ROCs and high AUCs >0.7. This serum over-expression positively correlated with the clinicopathological characteristics of both HCC and non-HCC patients. Conclusion: Serum miR-1246 level was significantly higher in HCC patients compared with non-HCC and HC and reliably discriminate early-stage HCV-related HCCs particularly when combined with PIVKA-II.]]>Jemal, A., F. Bray, M.M. Center, J. Ferlay, E. Ward and D. Forman,2011616990Fitzmaurice, C., C. Allen, R.M. Barber, L. Barregard and Z.A. Bhutta et al.,20173524548Chan, A.W., J. Zhong, S. Berhane, H. Toyoda and A. Cucchetti et al.,20186912841293Bruix, J., M. Reig and M. Sherman,2016150835853Sanyal, A.J., S.K. Yoon and R. Lencioni,2010151422Llovet, J.M., J. Zucman-Rossi, E. Pikarsky, B. Sangro, M. Schwartz and M. Sherman,20162016Giannini, E.G., S. Marenco, G. Borgonovo, V. Savarino and F. Farinati et al.,20125613711379El-Serag, H.B., J.A. Marrero, L. Rudolph and K.R. Reddy,200813417521763Mohammed, M.A., N.M. Omar, S.A. Mohammed and A.G. Deiab,20172017Tunissiolli, N.M., M.M.U. Castanhole-Nunes, P.M. Biselli-Chicote, É.C. Pavarino and R.F. da Silva,201718863872Ha, M. and V.N. Kim,20142014Bartel, D.P.,2009136215233Negrini, M., M. Ferracin, S. Sabbioni and C.M. Croce,200712018331840Cortez, M.A. and G.A. Calin,20099703711Dingle, T.C., R.H. Sedlak, L. Cook and K.R. Jerome,20135916701672Khairy, A., I. Hamza, O. Shaker and A. Yosry,20161726992703Motawi, T.K., O.G. Shaker, S.A. El-Maraghy and M.A. Senousy,20152015Liao, J.M., X. Zhou, Y. Zhang and H. Lu,20121126242630Sakha, S., T. Muramatsu, K. Ueda and J. Inazawa,20162016Zhang, W.C., T.M. Chin, H. Yang, M.E. Nga and D.P. Lunny et al.,20162016Lin, S. and R.I. Gregory,201515321333Forner, A., R. Vilana, C. Ayuso, L. Bianchi and M. Sole et al.,20084797104Llovet, J.M., C. Bru and J. Bruix,199919329338Bruix, J. and M. Sherman,20054212081236Peltier, H.J. and G.J. Latham,200814844852Livak, K.J. and T.D. Schmittgen,2001-ΔΔCT method.]]>25402408Zekri, A.R.N., A.S.E.D. Youssef, E.D. El-Desouky, O.S. Ahmed, M.M. Lotfy, A.A.M. Nassar and A.A. Bahnassey,2016371227312286Qi, P., S.Q. Cheng, H. Wang, N. Li, Y.F. Chen and C.F. Gao,20112011Ahmed, E.K., S.A. Fahmy, H. Effat and A.H.A. Wahab,201938109117Chuma, M., H. Toyoda, J. Matsuzaki, Y. Saito and T. Kumada et al.,20192019Ng, K.T.P., C.M. Lo, N. Wong, C.X. Li and X. Qi et al.,201671982419839Chai, S., K.Y. Ng, M. Tong, E.Y. Lau and T.K. Lee et al.,20166420622076Wang, Y., C. Zhang, P. Zhang, G. Guo and T. Jiang et al.,2018716701679Moshiri, F., A. Salvi, L. Gramantieri, A. Sangiovanni and P. Guerriero et al.,201891535015364Liu, Y., L. Lai, Q. Chen, Y. Song and S. Xu et al.,201218855005510Fabbri, M., A. Paone, F. Calore, R. Galli and E. Gaudio et al.,2012109E2110E2116