International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2016.152.161RagabHalla Mohamed ShaabanHebatAllah Mohamed MaksoudNabila Abd El RadwanSamah Mohamed ElazizWafaa Abd HafezNesreen Hassan 3-42016123-4Background and Objective: This study examined the correlation between immunohistochemistry (IHC) expression of the Vascular Endothelial Growth Factor (VEGF) in primary breast carcinoma tissue and its concentration in blood samples of the same patients preoperatively and to the other established clinicopathological parameters namely, tumor size, grade, lymph node status, Estrogen Receptor (ER), Progesterone Receptor (PR) status and human epidermal growth factor receptor 2 (Her2-neu) score. The current study also stressed on whether the ELISA detection is more sensitive and effective in diagnosis of breast cancer. Materials and Methods: Serum concentration of VEGF was measured using Enzyme Linked Immune Sorbent Assay (ELISA) in 92 primary breast cancer patients and compared with 25 benign breast disease patients. Also, tissue expression of VEGF was measured by immunohistochemistry. Results: Serum VEGF levels were significantly elevated in patients with malignant breast cancer (p = 0.000). The median serum level in the malignant group was 579 pg mL1 and in the control group 200 pg mL1. On the other hand no correlation was found between concentrations of serum VEGF and clinicopathological parameters. A significant association was showed between VEGF expression and lymph node metastasis, tumor size more than 2 cm and Her2-neu status (p<0.0001). No significant association was found between VEGF and patient age, histology, grade, ER and PR status (p>0.05). All positive cases for VEGF with strong positivity (score ++) were grade 2 and 3. Conclusion: The VEGF is overexpressed in breast carcinomas compared to the benign breast disease. Tissue expression of VEGF can be used as a prognostic marker due to the significant association with the large tumor size, lymph node metastasis and positive Her2-neu status. Tissue expression of VEGF can be used as a prognostic marker due to the significant association with the large tumor size, lymph node metastasis and positive Her2-neu status.]]>Atlanta, G.A.,20162016Ferlay, J., I. Soerjomataram, M. Ervik, R. Dikshit and S. Eser et al.,20122012Ibrahim, A.S., H.M. Khaled, N.N.H. Mikhail, H. Baraka and H. Kamel,20142014Jacobs, E.J., H.S. Feigelson, E.B. Bain, K.A. Brady and C. Rodriguez et al.,20062006Srabovic, N., Z. Mujagic, J. Mujanovic-Mustedanagic, A. Softic, Z. Muminovic, A. Rifatbegovic and L. Begic,20132013Nico, B., V. Benagiano, D. Mangieri, N. Maruotti, A. Vacca and D. Ribatti,200823601607Ali, E.M., M. Sheta and M.A. El Mohsen,201147217224Raica, M., L. Mogoanta, A.M. Cimpean, A. Alexa and S. Ioanovici et al.,2008493742Filho, A.L., J.M. Lopes and F.C. Schmitt,20102010Cimpean, A.M., M. Raica, C. Suciu, D. Tatucu, S. Sarb and A.M. Muresan,200849303308Byrne, G.J., G. McDowell, R. Agarawal, G. Sinha, S. Kumar and N.J. Bundred,20072734813487Adams, J., P.J. Carder, S. Downey, M.A. Forbes and K. MacLennan et al.,20006028982905Fox, S., D.G. Generali and A.L. Harris,20072007Ghasemi, M., O. Emadian, F. Naghshvar, A. Bekhradnia, S. Abediankenari, L.V. Larijani and R. Moghimpour,201149776779Werther, K., I.J. Christensen, H.J. Nielsen and The Danish RANX05 Colorectal Cancer Study Group,200286417423Tavassoli, F.A. and P. Devilee,2003Pages: 432Pages: 432Elston, C.W. and I.O. Ellis,199119403410Maae, E., M. Nielsen, K.D. Steffensen, E.H. Jakobsen, A. Jakobsen and F.B. Sorensen,201159750760Al-Bassam, S.S.A., H.S. Kadhim and B.M. Khashman,20144291293Fox, S.B. and A.L. Harris,2004112413430Abdelbaki, L., S. El Gizawy, K. Abdalazeem, M.Z.E. Hafez, R. Bakry, E.M. El-Gezawy and K.A. Nasif,2012938463851Dvorak, H.F.,20022043684380James, R., G. Ramesh, L. Krishnamoorthy and K. Thriveni,201010189195Kut, C., F. MacGabhann and A.S. Pope,200797978985Linderholm, B.K., S. Gruvberger-Saal, M. Ferno, P.O. Bendahl and P. Malmstrom,200817484491Comsa, S., A.M. Compean, R. Ceausu, C. Suciu and R. Raica,201264409417Valkovic, T., F. Dobrila, M. Melato, F. Sasso, C. Rizzardi and N. Jonjic,2002440583588Shahi, P.K., A.S. Lovelle and G.P. Manga,200911138142Hina, M., M.I. Qadir, A. Khizar and M. Ali,20148917923Konecny, G.E., Y.G. Meng, M. Untch, H.J. Wang and I. Bauerfeind et al.,20041017061716Al-Harris, E.S., A.A. Al-Janabi, K.M. Al-Toriahi and A.A. Yasseen,20082910991104Schoppmann, S.F., D. Tamandl, L. Roberts, G. Jomrich and A. Schoppmann et al.,201021955960Wulfing, P., C. Kersting, H. Buerger, B. Mattsson and R. Mesters et al.,2003in situ.]]>9217201728Nakamura, Y., H. Yasuoka, M. Tsujimoto, Q. Yang and A. Tsukiyama et al.,200316309314Garvin, S.,20062006