International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2016.101.108Muhartono Sutyarso KanediM. 22016122Background: Mucoxinis believed to be a promising anticancer because it is known to inhibit cell proliferation. However, given study on mucoxin still very limited, the mechanism of the substances isolated from leaf extract of Rollinia mucosa in regulating and eliminating cancer cells has not fully understood. This study investigated the mucoxin mechanism in affecting proliferation, expression of p53 and cyclin D1 genes in the T47D breast cancer cells. Materials and Methods: The cell line samples were grouped into four referred to the hour of assays undertaken after mucoxin application, namely hour 0th, 24th, 48th and 72nd. Each group was given mucoxin of six different concentrations namely 0.00 μg mL1 as a control, 0.1×103, 0.5×103, 1×103, 5×103 and 10×103 μg mL1 with three replications. Cells proliferation assayed by flow cytometry technique using BrDU staining protocol, whereas the expression of p53 and cyclin D1 genes determined by quantitative PCR (qPCR). Results: Cell proliferation in each group significantly reduced by mucoxin treatment. Mucoxin enhance p53 gene expression in 48 h, while the expression of cyclin D1 supressed signifantly by mucoxin of 5×103 and 10×103 μg mL1 in 48 and 72 h. Simple regression analysis showed that cell proliferation decreased with the increase of p53 expression and the suppression of cyclin D1 gene, while p53 expression positively associated to cyclin D1 expression. Conclusion: Mucoxin can decrease the proliferation of T47D breast cancer cells by suppressing the expression of cyclin D1 mediated by p53 gene.]]>Kumar, V., N. Fausto and A. Abbas,20057th Edn.,pp: 269-342, 1129-1149pp: 269-342, 1129-1149Hortobagyi, G.N.,201217577584Wang, L.Q., N. Nakamura, M.R. Meselhy, M. Hattori and W.M. Zhao et al.,2000Annona montana.]]>4811091113Cerella, C., F. Radogna, M. Dicato and M. Diederich,20132013Formagio, A.S., C.A. Kassuya, F.F. Neto, C.R. Volobuff, E.K. Iriguchi, M.D.C. Vieira and M.A. Foglio,2013Annona dioica St. Hill.]]>2013Narayan, R.S. and B. Borhan,20067114161429Oberlies, N.H., V.L. Croy, M.L. Harrison and J.L. McLaughlin,19971157379Coothankandaswamy, V., Y. Liu, S.C. Mao, J.B. Morgan and F. Mahdi et al.,201073956961Matsui, Y., T. Takeuchi, Y. Kumamoto-Yonezawa, M. Takemura, F. Sugawara, H. Yoshida and Y. Mizushina,201011926Liu, Y.Q., X. Cheng, L.X. Guo, C. Mao and Y.J. Chen et al.,20122012Moghadamtousi, S.Z., H.A. Kadir, M. Paydar, E. Rouhollahi and H. Karimian,2014Annona muricata leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-κB.]]>2014Qian, J.Q., P. Sun, Z.Y. Pan and Z.Z. Fang,201581193411944Ravi, R., B. Mookerjee, Z.M. Bhujwalla, C.H. Sutter and D. Artemov et al.,2000143444Xu, H. and M.R. El-Gewely,200313429436Guimaraes, D.P. and P. Hainaut,2002848393Diehl, J.A.,20021226231Hinz, M., D. Krappmann, A. Eichten, A. Heder, C. Scheidereit and M. Strauss,19990/G1-to-S-phase transition.]]>1926902698Fridman, J.S. and S.W. Lowe,20032290309040Mollereau, B. and D. Ma,2014Drosophila.]]>1914211429Yu, Z., H. Wang, L. Zhang, A. Tang and Q. Zhai et al.,2009386607611Yuan, S.S.F., H.L. Chang, H.W. Chen, Y.T. Yeh and Y.H. Kao et al.,20037228532861Rachmawati, E., S. Karyono and H. Suyuti,2013Annonamuricata leaf on proliferation and appoptosis of HeLacellsmediated by p53].]]>272832Yang, H.Y., Y.Y. Wen, C.H. Chen, G. Lozano and M.H. Lee,20032370967107Brooks, C.L. and W. Gu,200621307315Ou, Y.H., P.H. Chung, T.P. Sun and S.Y. Shieh,20051616841695Bai, L. and W.G. Zhu,20062141153Ozaki, T. and A. Nakagawara,201139941013Aliouat-Denis, C.M., N. Dendouga, I. van den Wyngaert, H. Goehlmann and U. Steller et al.,20053627634Hwang, B.J., J.M. Ford, P.C. Hanawalt and G. Chu,1999p48 xeroderma pigmentosum gene is p53-dependent and is involved in global genomic repair.]]>96424428Zhou, J., J. Ahn, S.H. Wilson and C. Prives,200120914923