International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2015.186.196WidowatiWahyu WijayaLaura AgustinaDwi MurtiHarry FauziahNurul B. SumitroSutiman WidodoM. Aris BachtiarIndra 42015114Cancer is one of the leading causes of mortality and morbidity throughout the world. Since there are still some problems related to the conventional therapies for cancer treatment, it is critical to explore new more efficient therapy strategies. Mesenchymal Stem Cells (MSCs) are one of powerful tools for tissue engineering for regenerative medicine, as recent research aims to utilize MSCs for anti-cancer treatment. Our previous research demonstrated that Conditioned Medium from Whartons Jelly MSCs (WJ-MSCs-CM) significantly lowered cancer proliferation of various cancer cell lines. This research was performed to evaluate the tumoricidal property of cell lysate from WJ-MSCs from normoxia (WJMSCs-norCL) and hypoxia-treated WJMSCs (WJMSCs-hypoCL) on the proliferation of human cancer cells, including cervical (HeLa), liver (HepG2), ovarian (SKOV3) and oral squamous (HSC3) cancer cell lines compared to normal cells including mouse fibroblast (NIH3T3), human Mesenchymal Stem Cells (hMSCs), human fibroblast. The WJMSCs-norCL and WJMSCs-hypoCL have cytotoxic activity, reduce proliferation of various cancer cell lines with minimum inhibitory concentration (IC50) 21.094-95.928 μg mL1 and no cytotoxic to normal cells with IC50 409, 191-629, 799.738 μg mL1. The WJMSCs-norCL and WJMSCs-hypoCL inhibit proliferation in various cancer cell lines and are not toxic for normal cells.]]>Ayuzawa, R., C. Doi, R.S. Rachakatla, M.M. Pyle, D.K. Maurya, D. Troyer and M. Tamura,2009in vitro and in vivo.]]>2803137Chen, L., Y. Xu, J. Zhao, Z. Zhang and R. Yang et al.,20142014Doi, C., N. Egashira, A. Kawabata, D.K. Maurya and N. Otha et al.,20102010Doi, C., D.K. Maurya, M.M. Pyle, D. Troyer and M. Tamura,201012408417Dominici, M., K. le Blanc, I. Mueller, I. Slaper-Cortenbach and F. Marini et al.,20068315317Gauthaman, K., C.Y. Fong, S. Cheyyatraivendran, A. Biswas, M. Choolani and A. Bongso,2012in vitro.]]>11320272039Gjorgieva, D., N. Zaidman and D. Bosnakovski,20138310318Haque, N., M.T. Rahman, N.H.A. Kasim and A.M. Alabsi,20132013Ho, I.A.W., H.C. Toh, W.H. Ng, Y.L. Teo, C.M. Guo, K.M. Hui and P.Y.P. Lam,201331146155Jemal, A., R. Siegel, E. Ward, T. Murray, J. Xu and M.J. Thun,2007574366Jun, E.K., Q. Zhang, B.S. Yoon, J.H. Moon and G. Lee et al.,201415605628Khakoo, A.Y., S. Pati, S.A. Anderson, W. Reid and M.F. Elshal et al.,200620312351247Kim, N. and S.G. Cho,201328387402Kobayashi, M., L. Fitz, M. Ryan, R.M. Hewick and S.C. Clark et al.,1989170827845Komarova, S., Y. Kawakami, M.A. Stoff-Khalili, D.T. Curiel and L. Pereboeva,20065755766Kucerova, L., V. Altanerova, M. Matuskova, S. Tyciakova and C. Altaner,20076763046313Lavrentieva, A., I. Majore, C. Kasper and R. Hass,20102010Lin, H.D., C.Y. Fong, A. Biswas, M. Choolani and A. Bongso,201410573586Maurya, D.K., C. Doi, A. Kawabata, M.M. Pyle and C. King et al.,20102010Nekanti, U., S. Dastidar, P. Venugopal, S. Totey and M. Ta,20106499512Otsu, K., S. Das, S.D. Houser, S.K. Quadri, S. Bhattacharya and J. Bhattacharya,200911341974205Pawitan, J.A.,20142014Prasanna, S.J., D. Gopalakrishnan, S.R. Shankar and A.B. Vasandan,20102010Qiao, L., Z. Xu, T. Zhao, Z. Zhao and M. Shi et al.,200818500507Siegel, R., D. Naishadham and A. Jemal,2013631130Tamura, M., A. Kawabata, N. Ohta, L. Uppalapati, K.G. Becker and D. Troyer,201143947Vile, R., D. Ando and D. Kirn,2002910621067Wang, J., J. Shou and X. Chen,20001918431848Widowati, W., T. Mozef, C. Risdian and Y. Yellianty,2013Catharanthus roseus, Dendrophthoe petandra, Piper betle and Curcuma mangga extracts in breast cancer cell lines.]]>2137142Widowati, W., L. Wijaya, I. Bachtiar, R.F. Gunanegara and S.U. Sugeng et al.,201464348Widowati, W., L. Wijaya, T.L. Wargasetia, I. Bachtiar, Y. Yellianty and D.R. Laksmitawati,20133225230Widowati, W., L. Wijaya, H. Murti, H. Widyastuti and D. Agustina et al.,20157817Wolf, S.F., P.A. Temple, M. Kobayashi, D. Young and M. Dicig et al.,199114630743081Zheng, L., D. Zhang, X. Chen, Y. Li, W. Yuquan and Z. Xia,20122012Xia, X., T. Ji, P. Chen, X. Li and Y. Fang et al.,20112011