International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2011.25.35AliRola AlabsiAied M. Manaf AliAbdual Ainideris Rahamn OmarAbdual YousoffKhatijah 1201171Newcastle Disease Virus (NDV) is a member of the new genus Avulavirus within the family Paramyxoviridae. Interest in the use of NDV as an anticancer agent has arisen from the ability of the virus to selectively kill human tumor cells with limited toxicity to normal cells. The aim of this study was to examine the potential of local strain NDV AF2240 as an anticancer agent against brain tumor cell line, glioblastoma multiforme (DBTRG.05 mg) in vitro. The IC50 values for cytolytic effects of NDV strain AF2240 on DBTRG.05MG cell lines was determined using assay MTT assay. IC50 values was 460 HAU mL-1 and no significant cytolytic effect was observed on normal cell lines at the same titre. Further study using TUNEL assay showed that the mode of cell death in response to infection by NDV strain AF2240 on brain tumor cell lines was by apoptosis. However, analysis of the cellular DNA content using PI showed that the virus caused an increase in sub-G1 region (apoptosis peaks). Early apoptosis was observed 6 h post-inoculation by annexin V flow-cytometry method. From this study, it was concluded that NDV strain AF2240 is an effective anti-cancer agent in brain tumor cell lines in vitro. It has a great potential to be an effective anticancer due to the ability to kill cancer cells and not normal cells. The mode of cell death induced by this virus is apoptosis and its cytotoxicity increasing while increasing the titer of the virus and increasing of time.]]>Alexander, D.J.,19881988pp: 147-160pp: 147-160Aubry, J.P., A. Blaecke, S. Lecoanet-Henchoz, P. Jeannin and N. Herbault et al.,199937197204Chambers, P. and A.C.R. Samson,198050155166van Engeland, M., L.J. Nieland, F.C. Ramaekers, B. Schutte and C.P. Reutelingsperger,19983119Everts, B. and H.G. van der Poel,200512141161Freeman, A.I., Z. Zakay-Rones, J.M. Gomori, E. Linetsky and L. Rasooly et al.,200613221228Fried, J., A.G. Perez and B.D. Clarkson,197671172181Othman, F., A. Ideris, G. Motalleb, Z.B. Eshak abd A. Rahmat,2010121724Gao, N., M.J. Keane, T. Ong, J. Ye, W.E. Miller and W.E. Wallace,2001175217225Gavrieli, Y., Y. Sherman and S.A. Ben-Sasson,1992In situ via specific labeling of nuclear DNA fragmentation.]]>119493501Henson, J.W.,19991999Lin, W.L., G.D. Li, Q. Chen and H.M. Lu,20041929112915Lorence, R.M., K.W. Richard, B.B. Katubig, H.M. Reyes and A. Phuangsab et al.,19948612281233Mangiardi, J.R. and H. Kane,20032003Meyyappan, M.,20032003Nicoletti, I., G. Migliorati, M.C. Pagliacci, F. Grignani and C. Riccardi,1991139271279Niederhuber, J.E.,20062006Omar, A., A. Ideris, A.M. Ali, F. Othman and K. Yussoff et al.,200310412Reichard, K.W., R.M. Lorence, C.J. Cascino, M.E. Peeples and R.J. Walter et al.,199252448453Stahelin, B.J., U. Marti, M. Solioz, H. Zimmermann and J. Reichen,199851204208Stupp, R., W.P. Mason, M.J. van den Bent, M. Weller and B. Fisher et al.,2005352987996Szeberenyi, J., Z. Fabian, B. Torocsik, K. Kiss and L.K. Csatary,200310282288Telford, W.G., L.E. King and P.J. Fraker,1994172116Vermes, I., C. Haanen, H. Steffens-Nakken and C. Reutelingsperger,19951843951Wali, H.S.M.,20032003Yasuhara, S., Y. Zhu, T. Matsui, N. Tipirneni and Y. Yasuhara et al.,200351873885You, L., B. He, Z. Xu, F. McCormick and D.M. Jablons,20045226230Yusoff, K., W.S. Tan, C.H. Lau, B.K. Ng and A.L. Ibrahim,199625837844Zawawi, M.,20062006