International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2009.12.24AminAmr 1200951The present study investigated the chemopreventive effects
of the water extract of chlorella on the development and growth of DMBA-induced
mammary tumors. Female rats were daily administered vehicle control or
chlorella either at 0.5 g or at 1.0 g kg-1 body weight starting
at age of 35 days and continued to the end of the experiment. At age of
50 days breast tumor was induced by administering DMBA at 25 mg kg-1
body weight. Similar DMBA dose was administered to DMBA-alone group at
age of 50 days. As a control for chlorella treatment one group (chlorella-alone)
was administered chlorella at 1.0 g kg-1 body weight starting
at age of 35 days and continued to the end of the experiment. Animals
were then followed for 15 weeks. Effects of chlorella on the expression
of proliferating cell nuclear antigen (PCNA), p53 and estrogen receptor
(ER) were investigated in mammary tissues of control and experimental
groups using immunohistochemistry. Present data demonstrated that chlorella
treatment restored the normal expression levels of PCNA and ER. Chlorella
also significantly increased cell death as assessed by the terminal deoxynucleotidyl
transferase-mediated triphoshate nick-end labeling (TUNEL) analysis. In
conclusion, the protective role of chlorellas water extract against
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