International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2008.61.70in vivo Animal Model for Cervical Intra-Epithelial Neoplasia]]>BustamamAhmad IbrahimSiddig DeviNirmala HalkimMuhd Nazrul Al-ZubairiAdel S. SyamM.M. 3200843Cervical cancer is the second most common cancer of
female reproductive tracts. In developing countries, cervical carcinoma
is the leading cause of cancer fatality in women. Despite attempts to
lower the fatality rate, very few in vivo models are in place to
investigate this cancer. We therefore are able to develop an in vivo
animal model that is suitable to conduct such study. In our attempt to
secure an in vivo animal model for cervical cancer, the carcinogenic
property of diethylstilboestrol (DES) was exploited to establish a model
for Cervical Intraepithelial Neoplasia or carcinoma (CIN). Female Balb/C
mice were injected with several dosages of DES (i.p) during pregnancy
at day 13-18. Female offspring were reared and sacrificed at age of 48-54
days and the cervix tissues taken for histological evaluation using H
and E. The progression of the cancer and hence, disease state is monitored
by measuring serum IL-6 using an ELISA kit. Proliferative cell nuclear
antigen (PCNA) expressions were studied by implying immunohistochemical
techniques. All parameters with regards to CIN were compared to a control
group of treating the cancer using a used drug, cisplatin, used preferentially
to treat cervical cancer in humans. The results of this study revealed
that a significant difference in serum IL-6 concentration between DES-treated
group and control groups (p<0.05). CIN histological related lesions
was noticed to be prominently dominant in DES-treated animals whilst these
lesions were absent in control groups. In addition to that PCNA index
in DES-treated animal was found to be a significant different compared
to control group. The above findings indicate that DES could be utilized
and further exploited as cervical carcinogenesis initiator in animal models
to screen and study new potential anti-cervical cancer compounds in
vivo.]]>Anna, G.,200345S354S360Block, K., A.P.I. Kardana and H.S. Taylor,2000In utero diethylstilbestrol (DES) exposure alters Hox gene expression in the developing mullerian system.]]>1411011108Branca, M., M. Ciotti, C. Giorgi, D. Santini and L. Di-Bonito et al.,2007130223231Burghardt, E.,197361401CDC,20042004David, A.E., R.R. Rebeccah, L.G. Ama, T.J. Frank and M.A. Jeffrey,20006012671275Gabriella, F., O.R. Franco, M.L. Luigi, M. Nicola, F. Erika, L. Francesco, S. Giuseppe, B. Alessandro, M. Salvatore and S. Giovanni,2001726562661Risbridger, G.P., H. Wang, M. Frydenberg and G. Cunha,200114224432450Herbst, A.L., H. Ulfelder and D.C. Poskanzer,1971284878881Jeffrey, M., M. Peter and H. Leydouglas,19969329302935Kaufman, R.H. and E. Adam,2002in utero to diethylstilbestrol.]]>99197200Ma, L., G.V. Benson, H. Lim, S.K. Dey and R.L. Maas,1998197141154Magnusson, P.K.E., P. Lichtenstein and U.B. Gyllensten,200088698701Mark, R., R.G. Fielden, C.J. Halgren, C.S. Fong, J. Larry, C. Karen and R. Tim,2002in vitro and testicular gene expression.]]>14330443059Marselos, M. and L. Tomatis,19922811821189Morioka, T., M. Suzui, V. Nabandith, M. Inamine, Y. Aniya, T. Nakayama, T. Ichiba and N. Yoshimi,2005Terminalia catappa on azoxymethane-induced colon carcinogenesis in male F344 rats.]]>14101105Paul, F.L. and B. Tiffany,200521520Quaas, J., M. Budner and J. Heinrich,200071109115Sarina, S. and E.P. Beth,20046923952400Srivani, R. and B. Nagarajan,2003in vivo expression of interleukin-6 in uterine cervical cancer.]]>13331339Stepien, H., H. Lawnicka, S. Mucha, M. Wagrowska-Danilewicz, B. Stepien, A. Siejka and J. Komorowski,20067917411748Swan, S.H.,2000108793804Takeshi, K., A.M. Alea and R.C. Gerald,200413116391649Tjalma, W.A., J.J. Weyler, J.J. Bogers, C. Pollefliet and M. Baay et al.,200197223230Wei, L.H., M.L. Kuo, C.A. Chen, W.F. Cheng, S.P. Cheng, F.J. Hsieh and C.Y. Hsieh,2001824956William, J.H., S. William and D.M.S. Branham,2004Mesocricetus auratus) cervix.]]>7013061316