International Journal of Cancer Research1811-97271811-9735Academic Journals Inc.10.3923/ijcr.2008.127.136FieldR. VergheseM. T. WalkerL. PanalaV. ShackelfordL. BoatengJ. 4200844The aim of present study was to determine the effects
of feeding Wheat Germ Meal (WGM) at 5 and 10% and Wheat Germ Oil (WGO)
at 7% (normal fat) and 14% (high fat) on azoxymethane (AOM) induced aberrant
crypt foci in Fisher 344 male rats. Following a 1 week period of acclimatization,
rats were assigned to 6 groups and fed American Institute of Nutrition
93-Growth diet (AIN-93G) with 7% (C1) and 14% (C2) soybean oil (SBO),
AIN-93G diet with 7 and 14% WGO (instead of SBO) and Wheat Germ Meal (WGM)
C1+ 5 and 10% WGM. At 7 and 8 week of age all rats received subcutaneous
injections of azoxymethane (AOM) at 16 mg kg-1 body weight
and were killed at 17 week of age by CO2 asphyxiation. AOM-induced
Aberrant Crypt Foci (ACF) were counted. Results showed that ACF formation
in distal colons in rats fed 7 SBO, 14 SBO, 7 WGO and 14% WGO (Means±SEM)
were 99.2±15.9, 126.0±17.0, 68.2±0.4 and 79.7±1.9
respectively. In rats fed 5% WGM and 10% WGM the number of ACF were 23.75±1.11,
18.6±1.0, 75.2±2.39 and 49.6±2.2 in proximal and
distal colon. Total ACF reductions (%) compared to control in rats fed
5% WGM and 10% WGM were 25% and 47%, respectively. Glutathione S-transferase
(GST) activities were significantly (p<0.05) higher in treatment groups
(5 and 10% WGM) compared to control (7 and 14% SBO). These results indicated
that wheat germ and wheat germ oil significantly (p< 0.05) reduced
AOM induced ACF. Regular consumption of wheat germ and wheat germ oil
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