International Journal of Biological Chemistry1819-155x2152-2561Science International10.3923/ijbc.2017.17.22Datura metel Plant Leaves]]>DhawanDixon GuptaJeena 12017111Background: The bioactive components present in the Datura metel plant are known to be responsible for its medicinal properties, to cure many diseases like asthma and bronchitis. However, the extraction method for these bioactive components is not yet standardized. The present study was undertaken to compare the effect of using different extraction solvents to extract the active components like alkaloids, flavinoids, saponins, steroids and tannins from the dried leaves of the Datura plant. Methodology: To achieve this, different extracts from the plant leaves were made using soxhlet apparatus. The extraction solvents used were distilled water, methanol, acetone, chloroform, ethyl acetate and hexane. Phytochemical estimations, total phenol concentration, flavonoid concentration and antioxidant activity have been evaluated to compare the efficiency of different extraction solvents. Results: The results shows that using methanol as an extraction solvent works best for the extraction of various active phytochemicals with flavonoid concentration of 16.48±0.22 mg of quercetin equivalent/100 g of extract, phenol concentration of 8.493±0.21 mg gallic acid equivalent/100 g of extract and 41.2±0.64% 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition which is significantly higher than chloroform which results in the minimum extraction with flavonoid concentration of 6.71±0.44 mg of quercetin equivalent/100 g of extract, phenol concentration of 0.196±0.02 mg gallic acid equivalent/100 g of extract and 15.3% 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition. Conclusion: To best of our knowledge this is the first report that directly compares the 6 extraction solvents for the extraction of active components from the Datura plant leaves and shows that methanol should be the solvent of choice.]]>Duke, J.A. and E.S. Ayensu,1985Vol. 1,pp: 90-91pp: 90-91Dabur, R., M. Ali, H. Singh, J. Gupta and G.L. Sharma,2004Datura metel leaves.]]>59568570Sangwan, R.S. and H. Camefort,1983Datura cultured in vitro.]]>78473480Okwu, D.E. and E.C. Igara,2009Datura metel Linn leaves.]]>3277281Chopra, R.N., S.N. Nayar and I.C. Chopra,1956Vol. 91,Chopra, R.N., S.L. Nayar and L.C. Chopra,1986pp: 238-240pp: 238-240Yang, B.Y., R. Guo, T. Li, J.J. Wu and J. Zhang et al.,2014Datura metel L.]]>872634Abubakar, M., U. Suleiman, A. Frank and A. Ukwuani,2009Datura metel in rats.]]>2009Arjun, T.N., H. Sudhir, E. Saxena, A. Dayma, R.S. Raghuvanshi and R. Shah,2015416611671Soni, P., A.A. Siddiqui, J. Dwivedi and V. Soni,2012Datura stramonium L. as a potential medicinal tree: An overview.]]>210021008Murthy, B.K., S. Nammi, M.K. Kota, R.V.K. Rao, N.K. Rao and A. Annapurna,2004Datura metel (Linn.) seeds in normal and alloxan-induced diabetic rats.]]>919598Alabri, T.H.A., A.H.S. Al Musalami, M.A. Hossain, A.M. Weli and Q. Al-Riyami,2014Datura metel L.]]>26237243Satyavati, G.V. and M.K. Raina,1977Vol. 1,pp: 333-334pp: 333-334Javaid, A., S. Shafique and S. Shafique,2010Datura metel against parthenium weed.]]>2414261437Djibo, A. and S.B. Bouzou,200093294297, (In French)Kuganathan, N. and S. Ganeshalingam,2011Datura metel leaves and investigation of the acute toxicity on grasshoppers and red ants.]]>8107112Blois, M.S.,195818111991200Javaid, A. and A. Saddique,2012Macrophomina phaseolina by extracts of Datura metel.]]>2617151720Roy, S., S. Pawar and A. Chowdhary,2016in vitro cytotoxic and antioxidant activity of Datura metel Linn. and Cynodon dactylon Linn. extracts.]]>8123127Umamaheswari, M., K. AsokKumar, A. Somasundaram, T. Sivashanmugam, V. Subhadradevi and T.K. Ravi,2007109547551Vermillion, K., F.O. Holguin, M.A. Berhow, R.D. Richins and T. Redhouse et al.,2011Datura inoxia leaves with specific cytotoxic activities.]]>74267271Kamaraj, C., A.A. Rahuman, G. Elango, A. Bagavan and A.A. Zahir,2011Haemonchus contortus.]]>1093745Selvarathi, P. and V. Ramasubramanian,2010Datura metal L. on paper mill effluent and its impact on physicochemical characteristics of Lycopersicon esculentum Mill.]]>194100