International Journal of Biological Chemistry1819-155x2152-2561Science International10.3923/ijbc.2013.58.68Klebsiella pneumoniae
and Escherichia coli of Environmental Surfaces at Upper Egypt]]>M. AfifiMagdy 2201372This study was aimed to evaluate the rate of environmental contamination of
Extended-spectrum β-Lactamase (ESBL) and non ESBL-producing Klebsiella
pneumoniae (K. pneumoniae) and Escherichia coli (E. coli)
as well as the susceptibility to 13 antimicrobial agents. Moreover, this study
documents two antibiotic resistance genes as well as highlights their important
role in spreading ESBL-producing bacteria among environmental surfaces at upper
Egypt. A total of 227 environmental isolates, K. pneumoniae (n = 102)
and E. coli (n = 125) were recovered from 750 samples of 6 different
environmental surfaces at upper Egypt. ESBL production was observed in 80 isolates,
for overall prevalence of 56.25% with a predominance of K. pneumoniae
(45/80), followed by E. coli prevalence of 43.75% with a predominance
of (35/80). The resistance rate was higher among ESBL producers than non ESBL
producers. All of K. pneumoniae and E. coli ESBL producers were
found resistance (100%) to cephalothin, ampicillin, cefuroxime, cefataxine,
ceftriaxone and ceftazidime. The resistance rate (%) was higher in aztreonam
(93.3 and 100) and cefotaxime (95.5 and 91.42) then gentamicin (84.4 and 42.8)
ciprpfloxacin (77.7 and 68.5) followed by cotrimoxazole (46.6 and 60) of ESBL-producing
K and E strains, respectively. PCR analysis demonstrated that 80.3% of ESBL-K
and 66.6% of ESBL-E isolates harbored SHV β-lactamase followed by 66.6%
of ESBL-K and 0.0% of ESBL-E isolates harbored TEM β-lactamase.]]>Abdel-Hady, H., S. Hawas, M. El-Daker and R. El-Kady,2008Klebsiella pneumoniae in neonatal intensive care unit.]]>28685690Ahmed, S.H., E.A. Daef, M.S. Badary, M.A. Mahmoud and A.A. Abd-Elsayed,20092009Al-Sweify, M.A. and E.S.A. El-Zayat,2008Klebsiella pneumoniae, isolated from critical care units.]]>17916Arabaci, F., M. Oldacay and D. Berber,2009Escherichia coli and Klebsiella pneumoniae strains with the sensititre esbl antimicrobic susceptibility plates in a public hospital, Turkey.]]>9103107Bradford, P.A., C. Urban, N. Mariano, S.J. Projan, J.J. Rahal and K. Bush,1997Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC β-lactamase, and the loss of an outer membrane protein.]]>41563569CLSI,20062006Cooper, B.S., S.P. Stone, C.C. Kibbler, B.D. Cookson and J.A. Roberts et al.,2004Staphylococcus aureus (MRSA): Systematic review of the literature.]]>2004Cormican, M.G., S.A. Marshall and R.N. Jones,19963418801884Coudron, P.E., S.E. Moland and C.C. Sanders,1997Enterobacteriaceae at a veterans medical center: Seek and you may find.]]>3525932597Daef, E.A., S.A. Aly, S.A.S. El-Din, N.M. El Sherbiny and S.M. El-Gendy,2009182939Fam, N., V. Leflon-Guibout, S. Fouad, L. Aboul-Fadl and E. Marcon et al.,2011Escherichia coli clinical isolates in Cairo (Egypt), including isolates of clonal complex ST10 and clones ST131, ST73 and ST405 in both community and hospital settings.]]>176773Frederick, A.,2011Escherichia coli, it prevalence and antibiotic resistant in Malaysia: A mini review.]]>14753FHCPH,2010Enterobacteriaceae and fight against their spread.]]>2010Grover, S.S., M. Sharma, D. Chattopadhya, H. Kapoor, S.T. Pasha and G. Singh,2006Klebsiella pneumoniae: Emergence of high resistance against cefepime, the fourth generation cephalosporin.]]>53279288Guet-Revillet, H., A. Le Monnier, N. Breton, P. Descamps and H. Lecuyer et al.,2012Escherichia coli and Klebsiella spp?]]>40845848Gururajan, G., K.A. Kaliyaperumal and B. Ramasamy,2011Escherichia coli and Klebsiella species in a Chennai suburban tertiary care hospital and its antibiogram pattern.]]>6796804Murugan, S., Jayanthi, P.U. Devi and P.N. John,2011Escherichia coli in diabetic foot infection.]]>6609617Jayapradha, R., S. Murugesh, N. Mahesh and D. Brahatheeswaran,20072491495Jones, K. and S.B. Bradshaw,1997Klebsiella pneumoniae.]]>82663668Kohlenberg, A., F. Schwab and H. Ruden,2012140528534Lewis, J.S., M. Herrera, B. Wickes, J.E. Patterson and J.H. Jorgensen,20075140154021Livermore, D.M. and D.F.J. Brown,2001485964Mandour, S.M.,2002Klebsiella.]]>2002Mansouri, S., F. Norouzi, M. Moradi and N. Nakhaee,2011Pseudomonas aeruginosa producing and non-producing extended spectrum β-lactamases.]]>48593Mattar, S. and P. Martinez,2007112335NCCLS,20032003Pagani, L., M. Perilli, R. Migliavacca, F. Luzzaro and G. Amicosante,2000Klebsiella pneumoniae strains causing outbreaks in intensive care units in Italy.]]>19765772Pascual, A., E. Perea, M. Alvarez, M. Casal and J.G. de Lomas et al.,200757195200Perez-Perez, F.J. and N.D. Hanson,20024021532162Rayamajhi, N., S.G. Kang, D.Y. Lee, M.L. Kang and S.I. Lee et al.,2008124183187Shanthi, M. and U. Sekar,2010Escherichia coli and Klebsiella pneumoniae: Risk factors for infection and impact of resistance on outcomes.]]>584144Starlander, G. and A. Melhus,2012Klebsiella pneumoniae in an intensive care unit due to a contaminated sink.]]>82122124Van 't Veen, A., A. Van Der Zee, J. Nelson, B. Speelberg, J.A. Kluytmans and A.G. Buiting,2005Klebsiella pneumoniae strain associated with contaminated roll boards in operating rooms.]]>4349614967Yu, Y., S. Ji, Y. Chen, W. Zhou, Z. Wei, L. Li and Y. Ma,2007545357Zaoutis, T.E., M. Goyal, J.H. Chu, S.E. Coffin and L.M. Bell et al.,2005Escherichia coli and Klebsiella species in children.]]>115942949