International Journal of Biological Chemistry1819-155x2152-2561Science International10.3923/ijbc.2013.47.57Pseudomonas aeruginosa
Isolated from Environmental and Clinical Samples in Upper Egypt]]>AfifiMagdy M. SuelamI.I.A. SolimanM.T.A. El-GoharyM.G.S. 2201372The present study was performed to isolate, characterize and evaluate the prevalence
of Pseudomonas aeruginosa (P. aeruginosa) in 600 samples (environmental
and clinical) at Upper Egypt. Antibiotic sensitivity, biotyping and molecular
identification of P. aeruginosa isolates were determined, as well as
hemagram was done on all positive clinical cases. Out of examined 200 environmental
samples, 42 P. aeruginosa strains (21%) were isolated. Out of examined
400 clinical specimens, 70 and 16 P. aeruginosa strains (35 and 8%) were
isolated from sputum and urine, respectively. The Multi-Drug Resistant (MDR)
P. aeruginosa isolates gave resistance to a number of antimicrobial types
ranged from 4/14-10/14. The total antimicrobial resistance profile of P.
aeruginosa isolated from environmental and clinical samples ranged from
3.1% (colistin) to 95.3% (cephalothin, cefuroxime and cefoxitin). The total
mean of MIC of tested antimicrobials versus P. aeruginosa (n = 128) ranged
from 1.13±0.02 μg mL-1 (colistin) to 24.7±2.1
μg mL-1 (ceftriaxone). The prevalence of P. aeruginosa between
environmental and clinical samples showed significant correlation. Moreover,
significant correlation found in total antimicrobial resistance profile between
the tested antimicrobials except imipenem and aztreonam shown non significant
(p>0.05). PCR analysis of P. aeruginosa indicates that species specific
signature sequences were present in 100% of the tested isolates. The hospital
environment and healthcare personnel could serve as potential reservoirs of
P. aeruginosa in the study locality. The obtained results may help in
prevention and control strategies of P. aeruginosa infection in both
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