International Journal of Botany1811-97001811-9719Asian Network for Scientific Information10.3923/ijb.2017.37.42NooriMitra TalebiMahdi KalantarMehrnoosh 12017131Background and Objective: Nitrotoxins or nitroglycosides are aliphatic nitro compounds, which were detected in some legumes (Papilionoideae). They are important due to mammalian toxicities, attraction of pollinators or seed disperses and repulsion or inhibition of herbivores and microorganisms. Legumes nitrotoxins studies have importance from the phytochemistry, chemotaxonomy, domestic nutrition, ecological adaptations and chemodiversity aspects. In this study nitrotoxins of Acacia farnesiana (L.) Willd., Bauhinia purpurea L., Cassia aphylla Cav. and Prosopis stephaniana Kunth species were reported for the first time that is important for their chemotaxonomy, toxicity and pollination. Materials and Methods: Thirty populations of 13 legume species were collected and identified from various parts of Iran. Their dried leaves analyzed for presence of aliphatic nitro compounds. The qualitative test and quantitative determination for aliphatic nitrotoxins were done using the developed Cooke and modified Williams-Parker methods. Recording the absorption spectrum between 400 and 800 nm was done using Cecill 4400 UV-visible double beam scanning spectrophotometer. Results: Nitrotoxins were detected in 5 species (Acacia farnesiana, Bauhinia purpurea, Cassia aphylla, Prosopis stephaniana and Robinia pseudoacacia) at concentrations ranging from 9-25 NO2 mg g1 plant. Other examined plant species lacked any nitrotoxins. Conclusion: Nitro compounds studies can show plant chemodiversity throughout the Papilionoideae as chemotaxonomic, toxicity and pollination character.]]>Kutchan, T.M.,20011255860Noori, M., A. Chehreghany and A. Hatami,200789479485Majak, W.,200154494498Williams, M.C.,1981Astragalus.]]>29261269Marsh, C.D. and A.B. Clawson,19201920James, L.F., W.J. Hartley and K.R. Van Kampen,1981178146150Majak, W. and M.A. Pass,19891989pp: 143-159pp: 143-159Noori, M.,2014Coronilla varia L.).]]>2014Noori, M., F. Amini and M. Foroghi,2011Coronilla varia L. nitrotoxins as defensive secondary metabolite against heavy metals pollution.]]>2011Noori, M., R. Poorimani and M. Khodaee,2011Coronilla varia L.]]>2011Noori, M. and A. Hatami,2012Papilionoideae (Leguminosae).]]>2012Patocka, J., J. Bielavsky, J. Cabal and J. Fusek,200043913Benchadi, W., H. Haba, C. Lavaud, D. Harakat and M. Benkhaled,2013Astragalus cruciatus Link. and their chemotaxonomic significance.]]>7105113Williams, M.C. and A.M. Davis,1982Astragalus species.]]>35113115Ebrahimzadeh, H., V. Niknam and A.A. Maassoumi,1999Astragalus species from Iran.]]>27743751Majak, W., M.H. Benn and Y.Y. Huang,1988Astragalus miser var. serotinus.]]>51985988Stermitz, F.R., F.A. Norris and M.C. Williams,19699145994600Williams, M.C. and E. Gomez-Sosa,1986Astragalus (Fabaceae) in Argentina.]]>39341344Anderson, R.C., W. Majak, M.A. Rassmussen, T.R. Callaway, R.C. Beier, D.J. Nisbet and M.J. Allison,20055323442350Moyer, B.G., P.E. Pfeffer, J.L. Moniot, M. Shamma and D.L. Gustine,1977Coronilla varia.]]>16375377Majak, W. and R.J. Bose,1976Coronilla varia.]]>15415417Hutchins, R.F.N., O.R.W. Sutherland, C. Gnanasunderam, W.J. Greenfield, E.M. Williams and H.J. Wright,1984Costelytra zealandica) (Coleoptera: Scarabaeidae).]]>108193Williams, M.C.,1981Indigofera species.]]>73434436Hipkin, C.R., M.A. Salem, D. Simpson and S.J. Wainwright,1999Hippocrepis comosa): A novel plant enzyme.]]>340491495Noori, M., B.E. Malayeri and M. Jafari,20109297105Ebrahimzadeh, H., A.A. Maassoumi and V. Niknam,20008213222Cooke, A.R.,1955Indigofera endecaphylla.]]>55114120Williams, M.C. and R. Parker,1974Astragalus.]]>22259262Williams, M.C. and R.C. Barneby,1977Astragalus (Fabaceae).]]>29310326