International Journal of Agricultural Research1816-48972152-2553Academic Journals Inc.10.3923/ijar.2007.152.158in and ex vitro Rooting of Iranian Myrtle]]>ShekafandehA. 2200722Nodal segments of wild Iranian myrtle was used to study the effects of two carbohydrate sources on shoot proliferation and to determine residual effect of different growth regulators and carbohydrate sources on in and ex vitro rooting. Half strength Murashige and Skoog (MS) medium was used for both shoot proliferation and rooting. The highest shoot proliferation (27.3 shoots/explant) observed in medium containing 2 mg L-1 b-benzylamino purine (BA) with 0.2 mg L-1 Indole-3-acetic acid (IAA) (M7) or 0.2 mg L-1 α-naphthaleneacetic acid (NAA) (M5). There were no significant differences between two sources of carbohydrates on shoot proliferation at all growth regulators levels. Maximum root numbers/shoot (3.8) observed on shoots originating from media containing 2 mg L-1 BA and 0.2 mg L-1 NAA on rooting media containing 0.5 mg L-1 IAA. The rate of rooting increased from zero% in non treated shoots to 91.7% when the basal end of shoots were dipped in a solution of 1.5 mg L-1 IAA and 0.31 mg L-1 Indole-3-butyric acid (IBA) for 24 h before culturing in soil mixture. A procedure for ex vitro rooting was proposed in order to decrease the micropropagation cost.]]>Ainsley, P.J., G.G. Collins and M. Sedgely,2001In vitro rooting of almond (Prunus dulcis Mill.).]]>37778785Collet, G.F.,1988in vitro.]]>277318323Debergh, P.C. and L.J. Maene,198114335345Fuentes, S.R.L., M.B.P. Caiheiros, J. Manetti-Filho and L.G.E. Viefra,2000Coffea canephora.]]>60513George, E.F.,1996Hatzilazarou, S., A.S.H. Grammatikos, N. Rifaki and P. Ralli,2003in vitro and acclimatization of myrtle communis micro cutting.]]>616259264Hazarika, B.N.,20038517041712Khosh-Khui, M., A. Shekafandeh and H. Azarakhsh,198422139146Magherini, R.,198831722Martin, K.P.,2003in vitro multiplication and ex vitro rooting of Rotula aquatica Lour. A rare rhoeophytic woody medicinal plant.]]>21415420Murashige, T. and F. Skoog,196215473497Nobre, J.,1994In vitro shoot proliferation of Myrtus communis L. from field-grown plants.]]>58253258Parra, R. and J.B. Amo-Marco,1998in vitro shoot proliferation of Myrtus communis L. a comparison of adult and seedling material.]]>34104107Parra, R. and J.B. Amo-Marco,1996in vitro shoot proliferation and rooting of Myrtus communis L.]]>38161168Peterson, K.K., J. Hansen and P. Krogstrup,1999Miscanthus H ogiformis Honda Giganteus.]]>58189197Ruffoni, B., M. Airo, G. Pascella, C., Mascarello, G., Zizzo and C. Cervelli,200316255258Salih, F.M. and M.T. Nadir,198455238241Scarpa, G.M., M. Milia and M. Satta,2000in vitro propagated myrtle.]]>62175176Torres, K.C.,1988pp: 285pp: 285Wainwright, H. and J. Scrace,1989in vitro preconditioning with carbohydrates during the rooting of microcutting on in vivo establishment.]]>38261267