International Journal of Agricultural Research1816-48972152-2553Academic Journals Inc.10.3923/ijar.2006.14.19Citrus reticulata BlancoxC. limetta Swing.)]]>DejamM. Khosh-KhuiM. ShekafandehA. 1200611Experiments were conducted to study the in vitro propagation of Bakrai using epicotyl explants. Explants were excised form in vitro grown seedlings, cultured on a basic MS medium supplemented with various concentrations of benzyladenine (BA) and naphthaleneacetic Acid (NAA). The highest percentage of explants producing normal adventitious shoots (83.3%) was obtained on a medium MS medium containing 2 mg L-1 BA. The maximum number of adventitious buds (20.33) produced in 4 mg L-1 BA, but most of these buds remained quiescent. Addition of 0.5 mg L-1 NAA increased shoot number and shoot length but higher NAA concentrations had an inhibitory effect on shoot regeneration, particularly in 1 mg L-1 BA concentration. The highest number of shoots (5.5) and longest shoots (9.68 mm) were obtained in concentrations of 2 and 1 mg L-1 BA plus 0.5 mg L-1 NAA, respectively. The shoots were rooted on a basal medium with half-strength MS salts and various concentrations of indolebutyric acid (IBA) (0.5-10 mg L-1 ). The highest percentage of rooting (91.67%) and the maximum number (1.71) and length (20.33 mm.) of roots were obtained with 10 mg L-1 IBA. The plantlets were transferred to pots containing an equal mixture (v/v) of sand and loamy soil and successfully acclimatized to natural environment with a survival rate of 80%.]]>Almeida, W.A.B., F.A.A. Mourao-Filho, B.M.J. Mendes and A.P.M. Rodrigues,2002In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia.]]>593540Barlass, M. and K.G.M. Skene,1982In vitro plantlet formation from Citrus species and hybrids.]]>17333341Bespalhok-Filho, J.C., A.K. Kobayashi, L.F.P. Pereira, Z. Hissano and L.G.E. Vieira,2001In vitro adventitious shoot regeneration from sweet orange using epicotyl sections.]]>12734Bhat, S.R., I. Chitralekha and K.P.S. Chandel,1992Citrus aurantifolia (Christm.) swing.]]>291925Burger, D.W. and W.P. Hackett,198643229232Costa, M.G.C., V.S. Alves, E.R.G. Lani, P.R. Mosquim, C.R. Carvalho and W.C. Otoni,20041006374Dejam, M., M. Khosh-Khui and A. Shekafandeh,2002In vitro propagation of sweet orange (Citrus sinensis L. Osbeck) by direct organogenesis in epicotyl segments.]]>28594Duran-Vila, N., V. Ortega and L. Navarro,198916123133Edriss, M.H. and D.W. Burger,1984In vitro propagation of Troyer citrange from epicotyl segments.]]>23159162Ghorbel, R., L. Navarro and N. Duran-Vila,1998Citrus paradisi), sour orange (C. aurantium) and alemow (C. macrophylla).]]>73323327Gmitter, F.G., J.W. Grosser and G.A. Moore,19921992pp: 335-369pp: 335-369Jackson. D.I. and N.E. Looney,1999pp: 321pp: 321Kitto, S.L. and M.J. Young,1981In vitro propagation of Carrizo citrange.]]>16305306Kobayashi, A.K., J.C. Bespalhok L.F.P. Pereira and L.G.E. Vieira,2003Citrus sinensis) from thin sections of mature stem segments.]]>7499102Kotsias, D. and P.A. Roussos,2001in vitro shoot tip and node culture of lemon seedlings.]]>89115128Maggon, R. and B.D. Singh,1995Citrus sinensis L. Osbeck).]]>63123128Moore, G.A.,1986In vitro propagation of citrus rootstocks.]]>21300301Moreira-Dias, J.M., R.V. Molina, Y. Bordon, J.L. Guardiola and A. Garcia-luis,200085103110Murashige, T. and F. Skoog,196215473497Normah, M., N.S. Hamidah and F.D. Ghani,1997Citrus halimii-an endangered species of South-East Asia.]]>50225227Perez-Molphe-Balch, E. and N. Ochao-Alejo,1997In vitro plant regeneration of Mexican lime and mandarin by direct organogenesis.]]>32931934Sim, G.E., C.J. Goh and C.S. Loh,1989Citrus mitis balanco-multiple bud formation from shoot and root explants in the presence of 6-benzylamino purine.]]>59203210Singh, S., B.K. Ray, S. Bhattacharyya and P.C. Deka,1994In vitro propagation of Citrus reticulata Blanco and Citrus limon Burm.f.]]>29214216Satoun, A., A. Mouras and A. Lutz,198257227231Wutscher, H.K.,19791237269