Bacteriology Journal2153-02112153-0238Science International10.3923/bj.2012.79.89DadaOlujimi KalilMohd. Sahaid YusoffWan Mohtar Wan 4201224The availability of ricebran; one of the by products of the rice-milling industry
in cheap and large quantity, is utilised in this work for the production of
renewable fuel through fermentation process. Treated ricebran, a lignocellulosic
material considered as wastes from the rice milling industry was fermented anaerobically
in a batch process using Clostridium saccharoperbutylacetonicum N1-4
as the inoculum at initial pH of 6±0.2 and temperature of 30°C for
the determination of the most suitable initial concentration of ricebran and
the inoculum concentration that will produce the maximum amount of products.
Different ricebran initial concentrations, 10, 20, 50, 100 and 120 g L-1
were fermented using inoculum concentration of 1.5 g L-1. Effect
of Inoculum concentration was also investigated by using inoculum concentrations
of 0.5, 1.0, 1.5, 2.0 and 2.5 g L-1 for fermentation of ricebran
hydrolysates at fixed substrate concentration and volume. Increment in substrate
concentration had a significantly positive effect (p<0.05) on yield and productivity
of acetone, butanol and ethanol up till concentration of 100 g L-1
after which further increment led to reduction in the yield and productivity.
In addition, increment in inoculum concentration also had a significantly positive
effect (p<0.05) on yield and productivity of the fermentation products up
till concentration of 1.5 g L-1 after which further increment in
inoculum concentration led to decrease in the yield and productivity. The highest
products(acetone, butanol and
ethanol) yield and productivity; 0.39 g g-1 and 0.079 g L-1
h-1, respectively, were obtained at the initial ricebran concentration
of 100 g L-1. In addition, the highest products
(acetone, butanol and ethanol) yield and productivity; 0.39 g g-1
and 0.095 g L-1 h-1, respectively were obtained at an
inoculum concentration of 1.5 g L-1. Higher inoculum concentration
resulted in lower yield and productivity of acetone, butanol and ethanol. The
best inoculum concentration and substrate concentration for production of acetone,
butanol and ethanol from treated ricebran are 1.5 and 100 g L-1,
respectively.]]>Allen, J.C. and R.C. Hamilton,19891989pp: 23-30pp: 23-30Argun, H., F. Kargi, I.K. Kapdan and R. Oztekin,20083318131819Carvalho, E.B. and W.R. Curtis,1999Hyoscyamus muticus: Implications for reactor inoculation.]]>4287293Dong, H., W. Tao, Z. Dai, L. Yang, F. Gong, Y. Zhang and Y. Li,201212885100Ezeji, T.C. and H.P. Blaschek,20102010pp: 19-38pp: 19-38Ferchichi, M., E. Crabbe, W. Hintz, G.H. Gil and A. Almadidy,2005Clostridium saccharoperbutylacetonicum ATCC 27021.]]>21855862Ghose, T.K.,198759257268Jesse, T.W., T.C. Ezeji, N. Qureshi and H.P. Blaschek,200229117123Khamaiseh, E.I., M.S. Kalil, O. Dada, I. El-Shawabkeh and W.M. Wan Yusoff,2012Clostridium acetobutylicum NCIMB 13357.]]>1211601165Lee, J., E. Seo, D.H. Kweon, K. Park and Y.S. Jin,2009Clostridium beijerinckii NCIMB 8052.]]>19482490Lee, S.Y., J.H. Park, S.H. Jang, L.K. Nielsen, J. Kim and K.S. Jung, 2008101209228Meilgaard, M., G.V. Civille and B.T. Carr,1999Neelakandan, T. and G. Usharani,2009Saccharomyces cerevisiae.]]>48588Ounine, K., H. Petitdemange, G. Raval and R. Gay,1985Clostridium acetobutylicum.]]>49874878Panagiotopoulos, I.A., R.R. Bakker, T. de Verje, P.A.M. Classen and E.G. Koukios,20092009Qureshi, N., T.C. Ezeji, J. Ebener, B.S. Dien, M.A. Cotta and H.P. Blaschek,20089959155959Saunders, R.M.,19851465495Sutton, S.,20061236Wang, L., J. Patindol and Y.J. Wang,20052005