Bacteriology Journal2153-02112153-0238Science International10.3923/bj.2011.16.23Rhodobacter sphaeroides]]>JaaparSyaripah Za`imah Syed KalilMohd Sahaid AliEhsan AnuarNurina 1201111Effects of different parameters including age of inoculum, size of inoculum and headspace on hydrogen production were investigated in detail using Rhodobacter sphaeroides NCIMB 8253 in batch culture. The effects of aerobic and anaerobic conditions for growth of this bacterium were also studied. The growth of R. sphaeroides NCIMB 8253 in aerobic condition reached exponential phase faster as compared to the anaerobic condition. The maximum absorbance at 660nm for the aerobic culture was 20% higher than the cells in anaerobic condition. The lag phase has been shortened when same medium was used for inoculum and H2 production but the H2 yield was found 9.26% lower as compared when different media were used for inoculum and H2 production. It was found that inoculum age 24 h, 10% v/v inoculum size with initial pH 7 can be designated as optimum conditions for maximum H2 production.]]>Alalayah, W.M., M.S. Kalil, A.A.H. Kadhum, J. Jahim, A. Zaharim, N.M. Alauj and A. El-Shafie,2010Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564).]]>13674682Alalayah, W.M., M.S. Kalil, A.A.H. Kadhum, J.M. Jahim, S.Z.S. Jaapar and N.M. Alauj,20091214621467Arik, T., U. Gunduz, M. Yucel, L. Turker, V. Sediroglu and I. Eroglu,1996Rhodobacter sphaeroides O.U.001.]]>324172424Basak, N. and D. Das,2007233142Chang, C.Y., M.Y. Lue and T.M. Pan,2005Antrodia camphorata by HPLC method.]]>13338342Emily, L.W.T., J. Nandong and Y. Samyudia,2009931263130Eroglu, I., K. Aslan, U. Gunduz, M. Yucel and L. Turker,1999Rhodobacter sphaeroides in a column photobioreactor.]]>70103113Fang, H.H.P., H. Zhu and T. Zhang,2006Clostridium butyricum and rhodobacter sphaeroides. ]]>3122232230Fascetti, E., E. D'Addario, O. Todini and A. Robertiello,199823753760Ferchichi, M., E. Crabbe, W. Hintz, G.H. Gil and A. Almadidy,2005Clostridium saccharoperbutylacetonicum ATCC 27021.]]>21855862Bratbak, G. and I. Dundas,198448755757Jee, H., T. Ohashi, Y. Nishizawa and S. Nagai,198765153158Kars, G., U. Gunduz, M. Yucel, L. Turker and I. Eroglu,2006Nifd, Nifk and hups genes in Rhodobacter sphaeroides o.U.001 grown in media with different concentrations of molybdenum and iron.]]>3115361544Koku, H., I. Eroglu, U. Gunduz, M. Yucel and L. Turker,2002Rhodobacter sphaeroides. ]]>2713151329Koku, H., I. Eroglu, U. Gunduz, M. Yucel and L. Turker,2003Rhodobacter sphaeroides O.U. 001.]]>28381388Lay, J.J.,200174280287Levin, D., L. Pitt and M. Love,200429173185Misi, S.E.E., A. Ramli and F.H. Rahman,20111112971302Uyar, B., I. Eroglu, M. Yucel, U. Gunduz and L. Turker,20073246704677Wooshin, P., H.H. Seung, E.O. Sang, E.L. Bruce and K. Ins,20063944164420Jaapar, S.Z.S., M.S. Kalil and N. Anuar,2009Rhodobacter sphaeroides NCIMB 8253.]]>1212531259