Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2022.65.70Zainab MazhariBi Bi AgsarDayanand 22022212Background and Objective: Actinomycetes are important microorganisms that have an important role in the production of bioactive molecules due to their ability to decompose many organic materials. Because of this the present investigation focus on the exploration of different goldmine soil for the isolation of potential actinomycetes which can be further used for the synthesis of gold nanoparticles. The study aims to explore gold mine soil to isolate and characterize the variety of actinomycetes prevailing in the soils of the Hutti gold mines and Kolar gold mines which can be further used for the synthesis of gold nanoparticles. Materials and Methods: Soil samples were subjected to the isolation of actinomycetes. The isolates of actinomycetes were screened for the synthesis of gold nanoparticles in starch casein broth using aurium chloride as a substrate. Results: The presence of gold nanoparticles in the solution was confirmed by visual observation of colour and UV-Vis Spectrum at 400-700 nm. Further, the size of gold nanoparticles was illustrated by scanning electron microscopy (SEM). Conclusion: Gold mines are considered as a good source for isolating biodiverse microorganisms and this isolated microorganism can be explored for the synthesis of various bioactive molecules among them Gold nanoparticles is considered to be one of the most important bioactive molecules because of their various applications in a different field.]]>Agnihotri, M., S. Joshi, A.R. Kumar, S. Zinjarde and S. Kulkarni,2009Yarrowia lipolytica NCIM 3589.]]>6312311234Anderson, A.S. and E.M.H. Wellington,200151797814Azam, A., F. Ahmed, N. Arshi, M. Chaman and A.H. 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