Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2021.22.30Bacillus spp.]]>B. AlviarKaren Mae R. LumangayaKrizzia Christine O. AdajarJoan S. PedroMannix 12021201Background and Objective: Several species of Bacillus are plant growth-promoting rhizobacteria that can produce the auxin phytohormone Indole-3 acetic acid (IAA) which regulates plant growth and development. This study aimed to amplify key genes, patB and dhaS, two of the three component enzymes found in the IPyA biosynthetic pathway for IAA synthesis and conduct 16S rRNA sequence analysis of two Bacillus isolates from Fermented Plant Juice (FPJ) extracted from Madre de Cacao, Gliricidia sepium. Materials and Methods: Genomic DNA extraction was performed using the CTAB method followed by the amplification of IPyA pathway genes through a polymerase chain reaction. Sequence similarity search was performed using NCBI BLASTn and the protein structure was predicted using Phyre2. Results: The dhaS gene from isolate 1 (B. amyloliquefaciens) had a top nucleotide BLAST hit with B. velezensis strain FJAT-45028 and a translated BLAST hit with an aldehyde dehydrogenase protein. The resulting rank three models in protein structure prediction revealed dhaS which encodes for an enzyme responsible for the conversion of indole-3-acetaldehyde (IAAld) to IAA. Furthermore, phylogenetic trees generated using the Maximum likelihood method revealed the relationship of each isolate with its top five BLAST hits. Conclusion: These amplified IAA-related genes may be further utilized in optimizing phytohormone production that has the potential to improve the capability of Bacillus isolates as biofertilizers.]]>Fekete, T.,2014Bacillus Species and Related Genera Other Than Bacillus anthracis.]]>2014pp: 2410-2414pp: 2410-2414Moat, A.G., J.W. Foster and M.P. Spector,20024th Edn.,Pages: 715Pages: 715Radhakrishnan, R., A. Hashem and E.F. Abd_Allah,2017Bacillus: A biological tool for crop improvement through bio-molecular changes in adverse environments.]]>2017Yuan, J., Y. Ruan, B. Wang, J. Zhang, R. Waseem, Q. Huang and Q. Shen,2013Bacillus amyloliquefaciens NJN-6-enriched bio-organic fertilizer suppressed Fusarium wilt and promoted the growth of banana plants.]]>6137743780Reetha, S., G. Bhuvaneswari, P. Thamizhiniyan and T.R. Mycin,2014Pseudomonas fluorescens and Bacillus subtilis and enhance growth of onion (Allim cepa L.).]]>3568574Borriss, R.,201730211215Khalid, A., S. Tahir, M. Arshad and Z.A. Zahir,200442921926Mashiguchi, K., K. Tanaka, T. Sakai, S. Sugawara and H. Kawaide et al.,20111081851218517Kumla, J., N. Suwannarach, K. Matsui and S. Lumyong,20202020Vullings, H.G.B., J.H.B. Diederen, D. Veelaert and D.J. Van Der Horst,199945142153Shao, J., S. Li, N. Zhang, X. Cui and X. Zhou et al.,2015Bacillus amyloliquefaciens SQR9.]]>2015Spaepen, S., J. Vanderleyden and R. Remans,200731425448Kittell, B.L., D.R. Helinski and G.S. Ditta,1989Rhizobium meliloti.]]>17154585466Koga, J., K. Syōono, T. Ichikawa and T. Adachi,1994Enterobacter cloacae.]]>1209241247Patten, C.L. and B.R. Glick,2002Pseudomonas putida GR12-2 by tryptophan and the stationary-phase sigma factor RpoS.]]>48635642Brandl, M.T. and S.E. Lindow,1996Erwinia herbicola.]]>6241214128Pollmann, S., A. Müller and E.W. Weiler,20068326333Idris, E.E., D.J. Iglesias, M. Talon and R. Borriss,2007Bacillus amyloliquefaciens FZB42.]]>20619626Atashpaz, S., S. Khani, A. Barzegari, J. Barar, S.Z. Vahed, R. Azarbaijani and Y. Omidi,201079538542Sainz, M.J., J.M. González-Jartín, O. Aguín, J.P. Mansilla and L.M. Botana,20182018pp: 202-245,pp: 202-245,Wilfinger, W.W., K. Mackey and P. Chomczynski,199722474481Zhang, N., D. Yang, J.R.A. Kendall, R. Borriss and I.S. Druzhinina et al.,2016Bacillus amyloliquefaciens and Bacillus subtilis reveals evolutional traits for adaptation to plant-associated habitats.]]>2016Fan, B., J. Blom, H.P. Klenk and R. Borriss,2017Bacillus amyloliquefaciens, Bacillus velezensis, and Bacillus siamensis form an “operational group B. amyloliquefaciens” within the B. subtilis species complex.]]>2017Kelley, L.A., S. Mezulis, C.M. Yates, M.N. Wass and M.J. Sternberg,201510845858Vasiliou, V., M. Sandoval, D.S. Backos, B.C. Jackson and Y. Chen et al.,20132022231Pavlopoulos, G.A., T.G. Soldatos, A. Barbosa-Silva and R. Schneider,20102010Zhang, P., T. Jin, S.K. Sahu, J. Xu, Q. Shi, H. Liu and Y. Wang,20192019Castro-Guerrero, J., A. Romero, J.J. Aguilar, M.L. Xiqui, J.O. Sandoval and B.E. Baca,2012Azospirillum brasilense Sp7, expressed in wheat.]]>356139150Ryu, R.J. and C.L. Patten,2008Enterobacter cloacae UW5.]]>19072007208Kumar, S., G. Stecher, M. Li, C. Knyaz and K. Tamura,20183515471549Cho, A.,20122012