Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2020.10.17AzzamNashwa Fawzy Abd El Moez 12020191Background and Objectives: Several infectious diseases became a big threat worldwide after spreading of multidrug resistant pathogens. Rayab milk found to be rich source of lactic acid bacteria with potential probiotics activity. Objective of the study was to evaluate the probiotic activity of LAB strains isolated from commercially fermented Rayab milk and to compare API identification method for some LAB isolates versus 16S rDNA sequencing molecular method. Materials and Methods:Lactobacillus MRS agar and broth media were applied to selectively isolate LAB from 20 fermented Rayab milk samples. API systems were used for biochemical identification. 16S rDNA sequencing had been used to identify ten randomly selected LAB isolates. Probiotic properties of all isolated LAB were tested, acid tolerability at pH 2.0, 3.0 and ability to grow in the presence of 0.3% bile salts, inhibitory effect of 10 antibiotics and bacteriostasis effect on food pathogens. Results: Ninety-five LAB had been isolated from 20 Rayab milk samples, 45 isolates out of them had been identified using API systems and grouped into four main genera previous reports, 62.2% of 45 isolates (28 isolates) showed distinguish resistance for surviving on low pH conditions. Fifteen out of these 28 isolates showed a tolerance to 0.3% bile salt. Moreover, 38 (84.4%) LAB isolates exhibited inhibitory activity against Staphylococcus aureus strain, Listeria monocytogenes strain (A), Salmonella typhimurium strain and E. coli O157 strain. Highest inhibition influences against LAB were observed for gentamicin, tobramycin and neomycin. While the lowest inhibition influences were recorded for amoxicillin, ceftriaxone, cefoxitin and nalidixic acid. Conclusion: LAB isolates with potential probiotic activity can be used to promote health and to combat food borne diseases.]]>Asfour, H.A.E., I.M. Gamal and S.F. Darwish,2016624759König H. and J. Fröhlich,20172017pp: 3-41pp: 3-41Mohania, D., R. Nagpal, M. Kumar, A. Bhardwaj and M. Yadav et al.,20089190198Zhang, B., Y. Wang, Z. Tan, Z. Li, Z. Jiao and Q. Huang,20162914901499Ricci, A.P., M. Cirlini, A. Maoloni, D. Del Rio and L. Calani et al.,20192019Dowarah, R., A.K. Verma, N. Agarwal, P. Singh and B.R. Singh,20182018Lavanya, B., S. Sowmiya, S. Balaji and B. Muthuvelan,20112510Javed, I.,20092009Chahad, O.B., M. El Bour, P. Calo-Mata, A. Boudabous and J. Barros-Velàzquez,20121634454Abosereh, N.A., S.A. El Ghani, R.S. Gomaa and M.T. Fouad,2016153543Zhang, J., X. Zhang, L. Zhang, Y. Zhao, C. Niu, Z. Yang and S. Li,2014Lactobacillus plantarum strains isolated from Inner Mongolia ]]>24225235CLSI.,201727th Edn., CLSI Document M100.Tulumoglu, S., H.I. Kaya and O. Simsek,2014Lactobacillus fermentum strains isolated from tulum cheese.]]>30120125Gilliland, S.E., T.E. Staley and L.J. Bush,1984Lactobacillus acidophilus used as a dietary adjunct.]]>6730453051Rather, I.A., K.H. Choi, V.K. Bajpai and Y.H. Park,2015Lactobacillus plantarum YML009 on Influenza virus H1N1.]]>10475482Tulumoglu, S., Z.N. Yuksekdag, Y. Beyatli, O. Simsek, B. Cinar and E. Yaşar,2013243642Rajoka, M.S.R., H.M. Mehwish, M. Siddiq, Z. Haobin and J. Zhu et al.,2017Lactobacillus rhamnosus isolated from human milk.]]>84271280Mourad, K. and K. Nour-Eddine,2006In vitro preselection criteria for probiotic lactobacillus plantarum strains of fermented olives origin.]]>12732Jacobsen, C.N., V.R. Nielsen, A.E. Hayford, P.L. Moller and K.F. Michaelsen et al.,1999Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans.]]>6549494956Hyronimus, B., C. Le Marrec, A.H. Sassi and A. Deschamps,200061193197Ouoba, L.I.I., V. Lei and L.B. Jensen,2008121217224De Almeida Júnior, W.L.G., I. da Silva Ferrari, J.V. de Souza, C.D.A. da Silva, M.M. da Costa and F.S. Dias,20155396103Dasen, A., F. Berthier, R. Grappin, A.G. Williams and J. Banks,200394595607Tulini, F.L., L.K. Winkelströter and E.C.P. de Martinis,2013Lactobacillus paraplantarum FT259, a bacteriocinogenic strain isolated from Brazilian semi-hard artisanal cheese.]]>225763Asurmendi, P., M.J. García, L. Pascual and L. Barberis,2015Listeria monocytogenes by lactic acid bacteria isolated from brewer's grains used as feedstuff in Argentina.]]>612731Tadesse, G., E. Ephraim and M. Ashenafi,200551320Bassyouni, R.H., W.S. Abdel-All, M.G. Fadl, S. Abdel-All and Z. Kamel,2012929242930Moraes, P.M., L.M. Perin, A. Silva Júnior and L.A. Nero,201344109112