Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2013.183.188LiuW.Y. 42013124Recently, it was reported that PPAR delta (PPARδ, alias
PPARβ) serves as a key nuclear transcription factor regulating the expression
of myriad genes in placent nutrient absorption and energy metabolism (lipid
and glucose metabolism) which suggested it might be vital in placental development
and pregnancy. In order to investigate and identify potential genetic markers
in swine breeding, we designed two pairs of primers for Single Strand Conformation
Polymorphism (SSCP) analysis based on the complete mRNA coding sequences of
PPARδ gene and its 5- flanking region. The sequencing result showed the
amplification product of a pair of primers was polymorphic and one mutation
C→T was identified in the second intron of PPARδ gene. Before the study,
genetic polymorphisms of swine PPARδ gene were rarely reported. The present
study provided useful information for future research in relevant medicinal
genetics and animal breeding.]]>Asami-Miyagishi, R., S. Iseki, M. Usui, K. Uchida, H. Kubo and I. Morita,2004315497501Barak, Y., M.C. Nelson, E.S. Ong, Y.Z. Jones and P. Ruiz-Lozano et al.,19994585595Batista, F.A.H., D.B.B. Trivella, A. Bernardes, J. Gratieri and P.S.L. Oliveira et al.,20122012Bener, A., S. Darwish, A.O. Al-Hamaq, R.M. Mohammad and M.T. Yousafzai,20136103111Castillero, E., N. Alamdari, Z. Aversa, A. Gurav and P. Hasselgren,20132013Domenici, F.A., M.J. Brochado, A.L. Martinelli, S. Zucoloto, S.F.C. da Cunha and H. Vannucchi,2013529326331Hutter, S., J. Knabl, U. Andergassen and U. Jeschke,20132013Johnson, R.K., M.K. Nielsen and D.S. Casey,199977541557Komatsu, M., Y. Fujimori, Y. Sato, H. Okamura and S. Sasaki et al.,201081530550Kruger, M.C., M. Coetzee, M. Haag and H. Weiler,201049438449Lehrke, M. and M.A. Lazar,2005123993999Lockyer, P., J.C. Schisler, C. Patterson and M.S. Willis,20102411111119Maciejewska-Karlowska, A., M. Sawczuk, P. Cieszczyk, A. Zarebska and S. Sawczyn,20132013Martinez, N., M. Kurtz, E. Capobianco, R. Higa, V. White and A. Jawerbaum,201147112Matsuda, S., M. Kobayashi and Y. Kitagishi,20132013Motavallian, A., S. Andalib, G. Vaseghi, H. Mirmohammad-Sadeghi and M. Amini,201319239244Msalya, G., T. Shimogiri, S. Okamoto, K. Kawabe, M. Minezawa, T. Namikawa and Y. Maeda,200980520527Nagy, L., A. Szanto, I. Szatmari and L. Szeles,201292739789Reilly, S.M. and C. Lee,20085822631Robinson, E. and D.J. Grieve,2009122246263Rothschild, M., C. Jacobson, D. Vaske, C. Tullge and L. Wang et al.,199693201205Sahmani, M., A. Gholami, A. Azarkeivan, M. Darabi, M.H. Ahmadi, M.S. Sabet and R. Najafipour,201337564573Schoonjans, K., B. Staels and J. Auwerx,1996130293109Shalom-Barak, T., J.M. Nicholas, Y. Wang, X. Zhang and E.S. Ong et al.,2004241066110669Wagner, K.D. and N. Wagner,2010125423435Wang, G., L. Kong, P. Hu, J. Fu and A. Wang,20113818071812Wang, J., X. Guo, P. Wu, J. Song and C. Ye et al.,2013528328334Wang, L., Z. Teng, S. Cai, D. Wang, X. Zhao and K. Yu,20132013Wang, Y., C. Lee, S. Tiep, R.T. Yu, J. Ham, H. Kang and R.M. Evans,2003113159170Yang, J., H. Gong, W. Liu and T. Tao,2013618941902Zhang, R., J. Wang, R. Yang, J. Sun and R. Chen et al.,20132013